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Vol 26 No Supplementary Issue

Copyright (c) 2014 Manop Sriuttha1, Mallika Chantarangsee1, Bung-Orn Hemung1

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Characteristics of Tilapia Bone Powder and Calcium Extraction by Acid Solution

https://doi.org/10.14233/ajchem.2014.19024
Manop Sriuttha1
1Faculty of Applied Science and Engineering, Khon Kaen University, Nong Khai Campus, Nong Khai 43000, Thailand 2Indo-China Country International Trade and Economic Research Sector, Khon Kaen University, Nong Khai Campus, Nong Khai 43000, Thailand *Corresponding author: E-mail: bunhem@nkc.kku.ac.th, hemung3@gmail.com
Mallika Chantarangsee1
1Faculty of Applied Science and Engineering, Khon Kaen University, Nong Khai Campus, Nong Khai 43000, Thailand 2Indo-China Country International Trade and Economic Research Sector, Khon Kaen University, Nong Khai Campus, Nong Khai 43000, Thailand *Corresponding author: E-mail: bunhem@nkc.kku.ac.th, hemung3@gmail.com
Bung-Orn Hemung1
1Faculty of Applied Science and Engineering, Khon Kaen University, Nong Khai Campus, Nong Khai 43000, Thailand 2Indo-China Country International Trade and Economic Research Sector, Khon Kaen University, Nong Khai Campus, Nong Khai 43000, Thailand *Corresponding author: E-mail: bunhem@nkc.kku.ac.th, hemung3@gmail.com

Corresponding Author(s) : Manop Sriuttha1

Asian Journal of Chemistry, Vol 26 No Supplementary Issue

Abstract

Tilapia bone powder was prepared by hot water and alkaline treatments, autoclaved, dried and ground to be powders. Fourier-transform infrared spectroscopy indicated that tilapia bone powders consisted of inorganic matters, especially hydroxyapatite. The proximate analysis revealed that organic compounds, protein and fat were presented but they could be removed more effectively in alkaline treatment than that in water. Microstructure showed the corrosive surface of the sample treated with alkaline, while the compact surface was found in another one. Therefore, the alkaline-treated bone was selected for calcium extraction by different acids and the calcium content was estimated by the redox titration. Based on the extractability and feasibility in food application, an acetic acid was selected to evaluate the suitable concentration and extraction time. The results turned to be at 0.25 M for 48 h. When, the calcium from tilapia bone powder was extracted at this condition, the calcium content was 2,376 mg/L, as quantified exactly by the inductively coupled plasma-optical emission spectroscopy. The extracted calcium could activate the calcium-dependent enzyme, transglutaminase, suggesting the availability for biological reaction.

Keywords

Calcium Fish bone Transglutaminase Acid solutions.
Sriuttha1, M., Chantarangsee1, M., & Hemung1, B.-O. (2014). Characteristics of Tilapia Bone Powder and Calcium Extraction by Acid Solution. Asian Journal of Chemistry, 26(27), 103–106. https://doi.org/10.14233/ajchem.2014.19024
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