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Phytochemical Investigations and Biological Potential of Moringa oleifera Pods
Corresponding Author(s) : Jyoti Punia
Asian Journal of Chemistry,
Vol. 29 No. 6 (2017): Vol 29 Issue 6
Abstract
Fresh pods of Moringa oleifera were collected, shadow-dried and chopped into small pieces. These were then extracted using hot methanol by refluxing method. The obtained methanolic extract was divided into two parts. One part was mixed with silica gel (60-120 mesh size) and subjected to column chromatography which on elution with solvents of increasing polarity and their mixtures afforded five compounds. These isolated compounds were characterized as 22-tritetracontanone, 1-octacosanol, methyl tetratricontanoate, hexadecane and b-sitosterol with the help of spectroscopic and analytical techniques. Other part of the methanolic extract was further fractionated with different solvents viz., hexane, benzene, chloroform, ethyl acetate, acetone and water. These fractions and methanolic extract were screened for antifungal activity by poisoned food technique at 250, 500, 1000 and 2000 μg/mL concentrations against two phytopathogenic fungi. All the extract/fractions were more toxic against Rhizoctonia solani than Fusarium oxysporum.
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- M. Debashri and M. Tamal, Res. J. Recent Sci., 1, 94 (2012).
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References
M. Debashri and M. Tamal, Res. J. Recent Sci., 1, 94 (2012).
A. Wasim, D. Sengupta and A. Chowdhury, Interdiscip. Toxicol., 2, 1 (2012).
M.H. Mughal, G. Ali, P.S. Srivasta and M. Iqbal, Hamdard Med., 42, 37 (1999).
R. Paliwal, V. Sharma and S. Pracheta, Biol. Med., 3, 25 (2011).
S.K. Pal, P.K. Mukherjee and B.P. Saha, Phytother. Res., 9, 463 (1995); https://doi.org/10.1002/ptr.2650090618.
M.U. Dahot, Pak. J. Biochem., 21, 1 (1988).
K. Mehta, R. Balaraman, A.H. Amin, P.A. Bafna and O.D. Gulati, J.Ethnopharmacol., 86, 191 (2003); https://doi.org/10.1016/S0378-8741(03)00075-8.
D.I. Sánchez-Machado, J.A. Núñez-Gastélum, C. Reyes-Moreno, B. Ramírez-Wong and J. López-Cervantes, Food Anal. Methods, 3, 175 (2010); https://doi.org/10.1007/s12161-009-9106-z.
L.J. Fuglie, J. Gomez-Zambrano, R.J. Aleman, I.S. Hunter, M. Furst, J.P.Garcia Sa and P. Ogazi, The Miracle Tree, Moringa oleifera (No. 633.85/F958), Republica Dominicana, Centro Para El Desarrollo Agropecuario Y Forestal (1999).
A. Karmakar, S. Karmakar and S. Mukherjee, Bioresour. Technol., 101, 7201 (2010); https://doi.org/10.1016/j.biortech.2010.04.079.
P. Sharma, P. Kumari, M.M. Srivastava and S. Srivastava, Bioresour. Technol., 97, 299 (2006); https://doi.org/10.1016/j.biortech.2005.02.034.
J. Tuite, Plant Pathological Methods, Fungi and Bacteria, Burgess Publishing Co., Minneapolis, USA (1969).
R.K. Grover and J.D. Moore, Phytopathology, 52, 876 (1962).
F.D. Gunstone, J.L. Harwood and F.B. Padly, The Lipid Handbook, CRC Press, edn 2, p. 418 (1994).
A. Pollard, A.C. Chibnall and S.H. Piper, Biochem. J., 27, 1889 (1933); https://doi.org/10.1042/bj0271889.
J. Heilbron, A.H. Cook, H.M. Bunbuyr and D.H. Hey, Dictionary of Organic Compounds, Eyre and Spottiswoode, London (1965).