Comparison of Chemical Composition of Root and Rhizosphere Soil Extracts of Tagetes patula L.: GC-MS Analysis

Abstract

The chemical composition of root and rhizosphere soil extracts of plant of Tagetes patula L. was investigated by GC-MS for the identification of allelochemicals. The dried and powdered root material was subjected to Soxhlet extraction using methanol. The rhizosphere soil around the plant was also extracted with methanol. GC-MS analysis of the roots and rhizosphere soil extracts led to identification of 25 and 27 compounds, respectively. The major compounds identified in methanol root extract were 5-hydroxymethylfurfural (21.81 %), butanoic acid, 2,2-dimethyl-3-oxo-methyl ester (11.71 %), 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (12.68 %), 5-(4-acetoxy-1-butynyl)-2,2'-bithienyl (8.50 %). However in rhizosphere soil extract dimethylsulfoxonium formylmethylide (6.32 %), 2-phenylthieno[2,3-b]thiophene (9.34 %), hexadecanoic acid (16.64 %), a-terthienyl (5.81 %), stigmasterol (5.32 %), g-sitosterol (5.71 %) were present as major compounds.