Isolation and Characterization of Bioactive Compounds from Stem of Nyctanthes arbor-tristis Linn. and Effect of Different Fractions on Phytopathogens

Abstract

Phytochemical investigation of the stem of Nyctanthes arbor-tristis (Harsingar) resulted the isolation of 21a-hydroxyfriedel-4-(23)-en-3-one, b-sitosterol, 1-triacontanol, friedel-1-ene-3-one, pelargonic acid and lignoceric acid. Extract/fractions of stem of Nyctanthes arbor-tristis were evaluated for their antifungal activity against the two plant pathogenic fungi viz. Rhizoctonia solani and Mycogone perniciosa and three phytopathogenic bacteria viz. Xanthomonas axonopodis pv. citri, Pseudovorax sp. and Dickeya zeae. The results revealed that the chloroform and ethyl acetate fractions exhibited maximum % growth inhibition i.e. 83.96 and 82.29 % against Mycogone perniciosa fungus respectively. Methanol extract was found most effective against Xanthomonas axonopodis pv. citri and Pseudovorax sp. bacteria with 11 and 3.50 mm inhibition zone at 2000 μg/mL concentration.