Spectrophotometric Determination of Sulfacetamide Sodium in Pharmaceutical Preparation Using 8-Hydroxy-7-iodoquinoline-5-sulfonic Acid as Chromogenic Reagent

Abstract

A simple, rapid and precise process have been presented for the analysis of sulfacetamide sodium in pure form as well as in pharmaceutical dosage. The process is based on the diazotization reaction of sulfacetamide sodium with sodium nitrite in the presence of hydrochloric acid to form diazonium salt, which is coupled with 8-hydroxy-7-iodoquinoline-5-sulfonic acid in alkaline medium to form azo dye, showing absorption maxima at 490 nm. Calibration plot was linear over the concentration range 2-28 μg mL^-1 for sulfacetamide sodium with detection limit 1.9178 μg mL^-1. The molar absorptivity and Sandell’s sensitivity are 6.6357 × 10³ L mol^-1 cm^-1 and 3.8318 × 10^-2 μg cm^-2, respectively. The method was successfully applied for analysis of sulfacetamide sodium in eye drops and did not require any preliminary separation or treatment of the samples. The results agree favourably with the Bratton-Marshall’s method (standard method).