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Phytochemical Study, Antioxidant activity and Kinetic Behaviour of Flavonoids Fractions Isolated from Prunus persica L. Leaves

https://doi.org/10.14233/ajchem.2017.20057
Houcine Benmehdi1
1Laboratory of Chemistry and Environmental Sciences, Department of Biology, University of Tahri Mohamed-Bechar, B.P. 417, Bechar, Algeria 2Laboratory of LASNABIO, Department of Chemistry, University of Tlemcen, Tlemcen, Algeria 3Institute of Science and Technology, Department of Natural Sciences and Life, Salhi Ahmed University Center, Naama, Algeria *Corresponding author: E-mail: h_ben90@yahoo.fr
Khadija Fellah1
1Laboratory of Chemistry and Environmental Sciences, Department of Biology, University of Tahri Mohamed-Bechar, B.P. 417, Bechar, Algeria 2Laboratory of LASNABIO, Department of Chemistry, University of Tlemcen, Tlemcen, Algeria 3Institute of Science and Technology, Department of Natural Sciences and Life, Salhi Ahmed University Center, Naama, Algeria *Corresponding author: E-mail: h_ben90@yahoo.fr
Abdelillah Amrouche3
1Laboratory of Chemistry and Environmental Sciences, Department of Biology, University of Tahri Mohamed-Bechar, B.P. 417, Bechar, Algeria 2Laboratory of LASNABIO, Department of Chemistry, University of Tlemcen, Tlemcen, Algeria 3Institute of Science and Technology, Department of Natural Sciences and Life, Salhi Ahmed University Center, Naama, Algeria *Corresponding author: E-mail: h_ben90@yahoo.fr
Faiza Memmou1
1Laboratory of Chemistry and Environmental Sciences, Department of Biology, University of Tahri Mohamed-Bechar, B.P. 417, Bechar, Algeria 2Laboratory of LASNABIO, Department of Chemistry, University of Tlemcen, Tlemcen, Algeria 3Institute of Science and Technology, Department of Natural Sciences and Life, Salhi Ahmed University Center, Naama, Algeria *Corresponding author: E-mail: h_ben90@yahoo.fr
Hesna Malainine1
1Laboratory of Chemistry and Environmental Sciences, Department of Biology, University of Tahri Mohamed-Bechar, B.P. 417, Bechar, Algeria 2Laboratory of LASNABIO, Department of Chemistry, University of Tlemcen, Tlemcen, Algeria 3Institute of Science and Technology, Department of Natural Sciences and Life, Salhi Ahmed University Center, Naama, Algeria *Corresponding author: E-mail: h_ben90@yahoo.fr
Halima Dalile1
1Laboratory of Chemistry and Environmental Sciences, Department of Biology, University of Tahri Mohamed-Bechar, B.P. 417, Bechar, Algeria 2Laboratory of LASNABIO, Department of Chemistry, University of Tlemcen, Tlemcen, Algeria 3Institute of Science and Technology, Department of Natural Sciences and Life, Salhi Ahmed University Center, Naama, Algeria *Corresponding author: E-mail: h_ben90@yahoo.fr
Wahiba Siata1
1Laboratory of Chemistry and Environmental Sciences, Department of Biology, University of Tahri Mohamed-Bechar, B.P. 417, Bechar, Algeria 2Laboratory of LASNABIO, Department of Chemistry, University of Tlemcen, Tlemcen, Algeria 3Institute of Science and Technology, Department of Natural Sciences and Life, Salhi Ahmed University Center, Naama, Algeria *Corresponding author: E-mail: h_ben90@yahoo.fr

Asian Journal of Chemistry, Vol. 29 No. 1 (2017): Vol 29 Issue 1

Abstract

The present study was conducted to evaluate the antioxidant activity of flavonoids extract from the leaves of Prunus persica L. The selective extraction of flavonoids gave yields of 0.71, 1.5 and 4.8 % for the fractions ethyl ether, ethyl acetate and n-butanol, respectively. The antioxidant activity was investigated with three different methods: Bioautographic HPTLC test, 2,20-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and ferric reducing ability of plasma (FRAP) assay. It has been found that flavonoids revealed the positive reaction (yellow spots) on the TLC plates sprayed with DPPH methanol solution. Besides, the DPPH method showed that the fractions of flavonoids (bunanol, ethyl acetate and diethyl ether) exhibited a potent scavenging activity with IC50 = 0.22, 0.27 and 0.76 mg/mL, respectively. Furthermore, our findings revealed that the extracts under study provided higher reducing power.

Keywords

Prunus persica Phytochemical study Flavonoids Antioxidant activity Free radical.
Benmehdi1, H., Fellah1, K., Amrouche3, A., Memmou1, F., Malainine1, H., Dalile1, H., & Siata1, W. (2016). Phytochemical Study, Antioxidant activity and Kinetic Behaviour of Flavonoids Fractions Isolated from Prunus persica L. Leaves. Asian Journal of Chemistry, 29(1), 13–18. https://doi.org/10.14233/ajchem.2017.20057
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