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Vol. 29 No. 12 (2017): Vol 29 Issue 12

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Cloud Point Extraction and Determination of Trace Iron(III) in Urine Samples by Spectrophotometry and Flame Atomic Absorption Spectrometry

https://doi.org/10.14233/ajchem.2017.20848
Ahmed Fadhil Khudhair
Department of Chemistry, College of Science, University of Karbala, Karbala, Iraq
Mouyed Khudhair Hassan
Department of Chemistry, College of Science, University of Karbala, Karbala, Iraq

Corresponding Author(s) : Ahmed Fadhil Khudhair

aliahmed79f@yahoo.com, ahmedfadhilkhudhair@gmail.com

Asian Journal of Chemistry, Vol. 29 No. 12 (2017): Vol 29 Issue 12

Abstract

Cloud point technique used effectively for extraction and pre-concentration of iron(III) in the urine samples of occupational workers prior measured by using flame atomic absorption spectrometry and UV-visible spectrophotometer. The metal responds with benzidine as reagent in a non-ionic surfactant Triton X-114 medium. The main factors affecting cloud point extraction efficiencies, such as pH of sample solution, concentration of benzidine reagent, type of surfactant, concentration of Triton X-114, effect of salt out, influence of interferences and impact of equilibration temperature and time were studied. The calibration curve was linear in the range of 0.25-3.0 μg mL-1 with r2 = 0.9655 for UV-visible spectrophotometer at lmax 425 nm. The limit of detection was 0.25 μg mL-1. The relative standard deviation for six replicates was 3.071 %.

Keywords

Cloud point extraction Triton X-114 UV-visible spectrophotometer Iron(III) Benzidine Urine samples
Khudhair, A. F., & Hassan, M. K. (2017). Cloud Point Extraction and Determination of Trace Iron(III) in Urine Samples by Spectrophotometry and Flame Atomic Absorption Spectrometry. Asian Journal of Chemistry, 29(12), 2725–2733. https://doi.org/10.14233/ajchem.2017.20848
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