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Vol. 26 No. 24 (2014)

Copyright (c) 2014 Nawal A. Alarfaj1, Maha F. El-Tohamy1

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Sensitive Extractive Spectrophotometric Methods for Determination of Trovafloxacin Mesylate Using Bromothymol Blue and Eriochrome Black-T

https://doi.org/10.14233/ajchem.2014.18758
Nawal A. Alarfaj1
1Department of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia 2General Administrative of Medical Affairs, Zagazig University, Zagazig, Egypt *Corresponding author: Tel/Fax: +966 14772245; E-mail: nalarfaj@hotmail.com
Maha F. El-Tohamy1
1Department of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia 2General Administrative of Medical Affairs, Zagazig University, Zagazig, Egypt *Corresponding author: Tel/Fax: +966 14772245; E-mail: nalarfaj@hotmail.com

Corresponding Author(s) : Nawal A. Alarfaj1

Asian Journal of Chemistry, Vol. 26 No. 24 (2014)

Abstract

Two sensitive and selective extractive spectrophotometric methods have been developed for determination of trovafloxacin mesylate either in pure form or its pharmaceutical dosage forms as well as biological fluids. The developed methods are based on the reaction of the drug with bromothymol blue (method I) and eriochrome black-T (method II) in acidic medium. The formed ion-pair coloured complexes at pH 3.5 and 3.8 were extracted quantitatively using chloroform and the absorbance maxima of the organic layer were measured at lmax 415 and 530 nm for methods I and II, respectively. Beer's law was obeyed under optimum experimental conditions and revealed that the linear concentration ranges were 2-100 and 5-130 μg mL-1 with bromothymol blue and eriochrome-black T, respectively. The correlation coefficients were (r = 0.9991 and 0.9993) with lower detection limits 0.87 and 1.84 μg mL-1 and quantification limits 2 and 5 μg mL-1 for both methods, respectively. The proposed methods have been successfully applied for determination of trovafloxacin mesylate in pharmaceutical formulations and biological fluids. The interference of some common pharmaceutical additives was investigated and no interference was observed. The reliability and the performance of the proposed methods were validated according to ICH guidelines and the obtained results were statistically treated and compared with those obtained from a reported method.

Keywords

Trovafloxacin mesylate Extractive spectrophotometry Bromothymol blue Eriochrome black-T Biological fluids.
A. Alarfaj1, N., & F. El-Tohamy1, M. (2014). Sensitive Extractive Spectrophotometric Methods for Determination of Trovafloxacin Mesylate Using Bromothymol Blue and Eriochrome Black-T. Asian Journal of Chemistry, 26(24), 8633–8639. https://doi.org/10.14233/ajchem.2014.18758
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