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Vol. 26 No. 24 (2014)

Copyright (c) 2014 Na Hu1, Zhongyin Ji1, Aihong Wang1, Lijuan Han1, Jinmao You1, Yourui Suo1

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Determination and Quantitative Analysis of Fatty Acids from Hippophae rhamnoides L. Using Reversed-Phase HPLC with Fluorescence Detection and Atmospheric Chemical Ionization-Mass Spectrometry

https://doi.org/10.14233/ajchem.2014.17014
Na Hu1
1Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China 2Academy of Agriculture and Forestry, Qinghai University, Xining 810016, P.R. China 3University of the Chinese Academy of Sciences, Beijing 100049, P.R. China *Corresponding author: Tel/Fax: +86 971 6143857; E-mail: yrsuo@nwipb.cas.cn
Zhongyin Ji1
1Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China 2Academy of Agriculture and Forestry, Qinghai University, Xining 810016, P.R. China 3University of the Chinese Academy of Sciences, Beijing 100049, P.R. China *Corresponding author: Tel/Fax: +86 971 6143857; E-mail: yrsuo@nwipb.cas.cn
Aihong Wang1
1Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China 2Academy of Agriculture and Forestry, Qinghai University, Xining 810016, P.R. China 3University of the Chinese Academy of Sciences, Beijing 100049, P.R. China *Corresponding author: Tel/Fax: +86 971 6143857; E-mail: yrsuo@nwipb.cas.cn
Lijuan Han1
1Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China 2Academy of Agriculture and Forestry, Qinghai University, Xining 810016, P.R. China 3University of the Chinese Academy of Sciences, Beijing 100049, P.R. China *Corresponding author: Tel/Fax: +86 971 6143857; E-mail: yrsuo@nwipb.cas.cn
Jinmao You1
1Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China 2Academy of Agriculture and Forestry, Qinghai University, Xining 810016, P.R. China 3University of the Chinese Academy of Sciences, Beijing 100049, P.R. China *Corresponding author: Tel/Fax: +86 971 6143857; E-mail: yrsuo@nwipb.cas.cn
Yourui Suo1
1Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China 2Academy of Agriculture and Forestry, Qinghai University, Xining 810016, P.R. China 3University of the Chinese Academy of Sciences, Beijing 100049, P.R. China *Corresponding author: Tel/Fax: +86 971 6143857; E-mail: yrsuo@nwipb.cas.cn

Corresponding Author(s) : Na Hu1

Asian Journal of Chemistry, Vol. 26 No. 24 (2014)

Abstract

In the present study, a selective and sensitive method using 2-(11H-benzo[a]carbazol-11-yl) ethyl 4-methylbenzenesulfonate as labeling reagent based on pre-column derivatization for the quantitative analysis of fatty acids was developed and validated. The results indicated the proposed method exhibited excellent selectivity, sensitivity, reproducibility and applicability. Good linear correlations were obtained for each fatty acid with correlation coefficients greater than 0.9994. The values of LOQs and LODs ranged from 1.34 to 6.64 ng mL-1 and 0.42 to 1.82 ng mL-1, respectively. This method was successfully employed to analyze fatty acid composition in the seeds of Hippophae rhamnoides L. The results showed that seeds of Hippophae rhamnoides L. seeds are rich in fatty acids and the contents of unsaturated fatty acids are much higher than that of saturated fatty acids. In addition, the contents of total fatty acids, unsaturated fatty acids and each fatty acid are various among different origins. The analysis of fatty acids in seeds of Hippophae rhamnoides L. is important to the pharmacological research of Hippophae rhamnoides L.

Keywords

Fatty Acids Hippophae rhamnoides L. Pre-column fluorescence labelling HPLC-FLD APCI-MS.
Hu1, N., Ji1, Z., Wang1, A., Han1, L., You1, J., & Suo1, Y. (2014). Determination and Quantitative Analysis of Fatty Acids from Hippophae rhamnoides L. Using Reversed-Phase HPLC with Fluorescence Detection and Atmospheric Chemical Ionization-Mass Spectrometry. Asian Journal of Chemistry, 26(24), 8339–8343. https://doi.org/10.14233/ajchem.2014.17014
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