Issue

Vol. 26 No. 23 (2014)

Copyright (c) 2014 Sumera Javad1, Shagufta Naz2, Saiqa Ilyas1, Amna Tariq1, Farah Aslam2

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Optimization of the Microwave Assisted Extraction and Its Comparison with Different Conventional Extraction Methods for Isolation of Stevioside from Stevia rebaudiana

https://doi.org/10.14233/ajchem.2014.17031
Sumera Javad1
1Department of Botany, Lahore College for Women University, Lahore, Pakistan 2Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan *Corresponding author: E-mail: drsnaz31@hotmail.com
Shagufta Naz2
1Department of Botany, Lahore College for Women University, Lahore, Pakistan 2Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan *Corresponding author: E-mail: drsnaz31@hotmail.com
Saiqa Ilyas1
1Department of Botany, Lahore College for Women University, Lahore, Pakistan 2Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan *Corresponding author: E-mail: drsnaz31@hotmail.com
Amna Tariq1
1Department of Botany, Lahore College for Women University, Lahore, Pakistan 2Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan *Corresponding author: E-mail: drsnaz31@hotmail.com
Farah Aslam2
1Department of Botany, Lahore College for Women University, Lahore, Pakistan 2Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan *Corresponding author: E-mail: drsnaz31@hotmail.com

Corresponding Author(s) : Sumera Javad1

Asian Journal of Chemistry, Vol. 26 No. 23 (2014)

Abstract

Stevioside is a natural acaloric stevioside being extracted from Stevia rebaudiana but 150-200 times sweeter than sucrose. In present study a comparison was made between conventional methods of extraction i.e., soxhlet extraction and cold maceration and modern method of extraction i.e., microwave assisted extraction for the rapid and efficient extraction of stevioside from Stevia rebaudiana. It was found that microwave assisted extraction gave the maximum amount of stevioside (0.7658 mg/g of dry leaf powder) while using lesser time of extraction (120 seconds) with a little amount of solvent used (10 mL/g). Another advantage of the method was the maximum quantity of stevioside was obtained from water which is an environment friendly solvent.

Keywords

Extraction Stevioside Stevia rebaudiana.
Javad1, S., Naz2, S., Ilyas1, S., Tariq1, A., & Aslam2, F. (2014). Optimization of the Microwave Assisted Extraction and Its Comparison with Different Conventional Extraction Methods for Isolation of Stevioside from Stevia rebaudiana. Asian Journal of Chemistry, 26(23), 8043–8048. https://doi.org/10.14233/ajchem.2014.17031
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. J.M.C. Geuns, Phytochemistry, 64, 913 (2003).
  2. E.J. Kennelly, in ed.: A.D. Kinghorn, Stevia, The genus Stevia. Medicinal and Aromatic Plants-Industrial profiles, vol. 19, p. 68 (2002).
  3. D.D. Soejarto, in ed.: A.D. Kinghorn, Taylor and Francis, London and New York, p. 40 (2002).
  4. I.E. Brandle and P.G. Telmer, Phytochemistry, 68, 1855 (2007).
  5. M.S. Uddin, S.H.C. Mohammad, M.M.H.K. Mohammad, U. Belal, A. Romel and A.B. Mohammad, Afr. J. Bot., 5, 1238 (2006).
  6. A.S. Dacome, C.C. da Silva, C.E.M. da Costa, J.D. Fontana, J. Adelmann and S.C. da Costa, Process Biochem., 40, 3587 (2005).
  7. V.P. Tsanava, G.P. Sardzhveladze and L.G. Kharebava, Subtropic-heskieKul'Tury, 3, 64 (1991).
  8. A.M. Hutapea, C. Toskulkao, D. Buddhasukh, P. Wilairatand T. Glinsukon, J. Clin. Biochem. Nutr., 23, 177 (1997).
  9. A. Wheeler, A.C. Boileau, P.C. Winkler, J.C. Compton, I. Prakash, X. Jiang and D.A. Mandarino, Food Chem. Toxicol., 46, 54 (2008).
  10. T. Jain, V. Jain, R. Panday and A. Vyas, Asian J. Res. Chem., 2, 19 (2009).
  11. V. Mandal, Y. Mohan and S. Hemalatha, Pharmacog. Rev., 1, 7 (2007).
  12. D. Jyothi, S. Khanam and R. Sultana, Int. J. Pharm. Pharm. Sci., 2, 46 (2010).
  13. S. Pan, R. Aebersold, R. Chen, J. Rush, D.R. Goodlett, M.W. McIntosh, J. Zhang and T.A. Brentnall, J. Proteome Res., 8, 787 (2009).
  14. X. Chaudot, A. Tambute and M. Caude, J. Chromatogr. A, 866, 231 (2000).
  15. H. Sonnenschein, I. Germanus and P. Harting, Chem. Eng. Technol., 74, 270 (2002).
  16. E. Martino, I. Ramaiola, M. Urbano, F. Bracco and S. Collina, J. Chromatogr. A, 1125, 147 (2006).
  17. Y. Zhang, X.F. Li, G.S. Liu and Y.F. Chen, J. Northwest Sci-Tech. Univ. Agric. For. (Nat. Sci. Ed.), 35, 111 (2007).
  18. M. Soylak, M. Tuzen, A.S. Souza, M.G.A. Korn and S.L.C. Ferreira, J. Hazard. Mater., 149, 264 (2007).
  19. C. Deng, X. Xu, N. Yao, N. Li and X. Zhang, Anal. Chim. Acta, 556, 289 (2006).
  20. W.H. Xiao, L.J. Han and B. Shi, Sep. Purif. Technol., 62, 614 (2008).
  21. C. Sparr Eskilsson and E. Björklund, J. Chromatogr. A, 902, 227 (2000).
  22. B. Kaufmann, P. Christen and J. Veuthey, Phytochem. Anal., 12, 327 (2001).
  23. G. Raman and V.G. Gaikar, Ind. Eng. Chem. Res., 41, 2521 (2002).
  24. Y.Y. Shu, M.Y. Ko and Y.S. Chang, Microchem. J., 74, 131 (2003).
  25. J. Fornal and R. Jaroch, Fat Sci. Technol., 94, 192 (1992).
  26. M. Desai, J. Parikh and P.A. Parikh, Sep. Purif. Rev., 39, 1 (2010).
Read More
Author biographies is not available.
Download
Statistic
Read Counter : 0