Issue

Vol. 26 No. 20 (2014)

Copyright (c) 2014 Saad Antakli1, Nazeera Sarkees2, Tereza Sarraf1

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Determination of Water-Soluble Vitamins B1, B2, B3, B6, B9, B12 and C on ODS Column 5 μm by High Performance Liquid Chromatography with UV-DAD Detection

https://doi.org/10.14233/ajchem.2014.17814
Saad Antakli1
1Department of Chemistry, Faculty of Science, University of Aleppo, Aleppo, Syria 2Department of Analytical Chemistry, Faculty of Pharmacy, University of Aleppo, Aleppo, Syria *Corresponding author: E-mail: antakli@scs-net.org
Nazeera Sarkees2
1Department of Chemistry, Faculty of Science, University of Aleppo, Aleppo, Syria 2Department of Analytical Chemistry, Faculty of Pharmacy, University of Aleppo, Aleppo, Syria *Corresponding author: E-mail: antakli@scs-net.org
Tereza Sarraf1
1Department of Chemistry, Faculty of Science, University of Aleppo, Aleppo, Syria 2Department of Analytical Chemistry, Faculty of Pharmacy, University of Aleppo, Aleppo, Syria *Corresponding author: E-mail: antakli@scs-net.org

Corresponding Author(s) : Saad Antakli1

Asian Journal of Chemistry, Vol. 26 No. 20 (2014)

Abstract

HPLC method was developed and applied to the determination of some water-soluble vitamins (B1, B2, B3, B6, B9, B12 and C) in Syrian pharmaceutical preparations. Reserved phase, ion-pair high performance liquid chromatography with UV-DAD detection was employed. The developed chromatographic method employed an ODS column with dimension (150 × 4.6 mm; 5 μm). The mobile phase was 5.3 mM of hexane-1-sulfonic acid sodium with 0.1 % triethylamine as solvent (A) at pH 3.3 and 5.3 mM of hexane-1-sulfonic acid sodium:acetonitrile (50:50) with 0.1 % triethylamine as solvent (B) at pH 3.5 and paracetamol as internal standard. The method showed acceptable values for precision, recovery and sensitivity.

Keywords

Determination Vitamins Reserved phase Ion-pair.
Antakli1, S., Sarkees2, N., & Sarraf1, T. (2014). Determination of Water-Soluble Vitamins B1, B2, B3, B6, B9, B12 and C on ODS Column 5 μm by High Performance Liquid Chromatography with UV-DAD Detection. Asian Journal of Chemistry, 26(20), 7035–7039. https://doi.org/10.14233/ajchem.2014.17814
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