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Copyright (c) 2014 Guofang Xu1, Xiaohua Li1, Ping Liu1, Guanghui Li1, Zhihui Hua1, Xiaosu Li2
This work is licensed under a Creative Commons Attribution 4.0 International License.
Simultaneous Analysis of Four Bioactive Compounds in Migan Granules Using Internal Addition Standard Method in Gas Chromatography
Corresponding Author(s) : Guofang Xu1
Asian Journal of Chemistry,
Vol. 26 No. 20 (2014)
Abstract
A simple and reliable gas chromatography method with flame ionization detector was developed for the separation and quantification of four bioactive essential compounds including deoxyschizandrin, schizandrin B, schisandrol A and imperatorin in a traditional Chinese medicine preparation (TCMP), Migan granules. The analytes were distilled with steam distillation. The separation was achieved on an HP-5 phenyl methyl siloxane capillary column (30 m × 0.32 mm, i.d. × 0.50 μm) with temperature programmed. The LODs and LOQs of the four analytes were less than 2.10 and 6.93 μg/mL, respectively. The intra-day and inter-day coefficients of variation were less than 4.3 % (RSD) and the recoveries were in the range of 91.4-99.3 % for all four analytes. The validated method was successfully applied to simultaneously determine the four essential compounds in Migan granules.
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References
Chinese Ministry of Health, Standard for Chinese Patent Medicines, Part of Medical Renal System, No. WS-11401(ZD1401)-2002, Chemical Industry Press, Beijing, p. 26 (2003).
M. Zhu, K.F. Lin, R.Y. Yeung and R.C. Li, Ethnopharmacol., 67, 61 (1999).
P.Y. Chiu, M.H. Tang, D.H. Mak, M.K. Poon and K.M. Ko, Free Radic. Biol. Med., 35, 368 (2003).
S.P. Ip, M.K. Poon, C.T. Che, K.H. Ng, Y.C. Kong and K.M. Ko, Free Radic. Biol. Med., 21, 709 (1996).
P. Qiangrong, T. Wang, Q. Lu and X. Hu, Biochem. Biophys. Res. Commun., 335, 406 (2005).
Y. Wei and Y. Ito, J. Chromatogr. A, 1115, 112 (2006).
W. Ketai, L. Huitao, C. Xingguo, Z. Yunkun and H. Zhide, Talanta, 54, 753 (2001).
D. Lechner, M. Stavri, M. Oluwatuyi, R. Pereda-Miranda and S. Gibbons, Phytochemistry, 65, 331 (2004).
H. Sun, F. Wu, A. Zhang, W. Wei, Y. Han and X. Wang, J. Sep. Sci., 36, 485 (2013).
S. Zhu, Y. Wang, M. Chen, J. Jin, Y. Qiu, M. Huang and Z. Huang, Am. J. Chin. Med., 40, 551 (2012).
H. Wei, L. Sun, Z. Tai, S. Gao, W. Xu and W. Chen, Anal. Chim. Acta, 662, 97 (2010).