Issue

Vol. 26 No. 20 (2014)

Copyright (c) 2014 Yun Ling1, Yinjia Zheng3, Keyue Liu2, Qing Zhang2, Yunhua Lu1

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Structure Characterization and Identification of Triterpenoid Saponins from the Root of Platycodon grandiflorum by HPLC/ESI-QTOF-MS/MS

https://doi.org/10.14233/ajchem.2014.17018
Yun Ling1
1College of Life Sciences, Huzhou Teachers College, Huzhou 313000, P.R. China 2College of Life Sciences, Jiujiang University, Jiujiang 332005, P.R. China 3Department of Applied Psychology, School of Health Management, Guangzhou Medical University, Guangzhou 510182, P.R. China †These authors contributed equally to this work *Corresponding author: E-mail: lymanuxt @126.com
Yinjia Zheng3
1College of Life Sciences, Huzhou Teachers College, Huzhou 313000, P.R. China 2College of Life Sciences, Jiujiang University, Jiujiang 332005, P.R. China 3Department of Applied Psychology, School of Health Management, Guangzhou Medical University, Guangzhou 510182, P.R. China †These authors contributed equally to this work *Corresponding author: E-mail: lymanuxt @126.com
Keyue Liu2
1College of Life Sciences, Huzhou Teachers College, Huzhou 313000, P.R. China 2College of Life Sciences, Jiujiang University, Jiujiang 332005, P.R. China 3Department of Applied Psychology, School of Health Management, Guangzhou Medical University, Guangzhou 510182, P.R. China †These authors contributed equally to this work *Corresponding author: E-mail: lymanuxt @126.com
Qing Zhang2
1College of Life Sciences, Huzhou Teachers College, Huzhou 313000, P.R. China 2College of Life Sciences, Jiujiang University, Jiujiang 332005, P.R. China 3Department of Applied Psychology, School of Health Management, Guangzhou Medical University, Guangzhou 510182, P.R. China †These authors contributed equally to this work *Corresponding author: E-mail: lymanuxt @126.com
Yunhua Lu1
1College of Life Sciences, Huzhou Teachers College, Huzhou 313000, P.R. China 2College of Life Sciences, Jiujiang University, Jiujiang 332005, P.R. China 3Department of Applied Psychology, School of Health Management, Guangzhou Medical University, Guangzhou 510182, P.R. China †These authors contributed equally to this work *Corresponding author: E-mail: lymanuxt @126.com

Corresponding Author(s) : Yun Ling1

Asian Journal of Chemistry, Vol. 26 No. 20 (2014)

Abstract

Triterpenoid saponins, derived from the root of Platycodon grandiflorum, have been shown many biological activities, such as anti-inflammatory, antioxidant, antitumor, antiobesity and antihyperlipidemia effects. Nevertheless, the high polarity, non chromophores and low abundance of these compounds in this plant bring difficulty for their isolation and structural determination by traditional chromatographic and spectroscopic techniques. The aim of the present study was to develop a sensitive and rapid high-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (HPLC/ESI-QTOF-MS/MS) approach for the structural analysis and identification of triterpenoid saponins. According to the HPLC retention behaviour, the proposed fragmentation pathways provided by high-resolution MS and MS/MS spectra and literature sources, a total of 18 triterpenoid saponins in the crude extract of root of Platycodon grandiflorum were identified or tentatively characterized. This method therefore proved to be a very useful technique for the characterisation and structure elucidation of triterpenoid saponins of this plant.

Keywords

Triterpenoid saponins Platycodon grandiflorum HPLC/ESI-QTOF-MS/MS.
Ling1, Y., Zheng3, Y., Liu2, K., Zhang2, Q., & Lu1, Y. (2014). Structure Characterization and Identification of Triterpenoid Saponins from the Root of Platycodon grandiflorum by HPLC/ESI-QTOF-MS/MS. Asian Journal of Chemistry, 26(20), 6839–6844. https://doi.org/10.14233/ajchem.2014.17018
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Z.D. He, C.F. Qiao, Q.B. Han, Y. Wang, W.C. Ye and H.X. Xu, Tetrahedron, 61, 2211 (2005).
  2. W.W. Fu, N. Shimizu, D.Q. Dou, T. Takeda, R. Fu, Y.H. Pei and Y.J. Chen, Chem. Pharm. Bull. (Tokyo), 54, 557 (2006).
  3. T. Nikaido, K. Koike, K. Mitsunaga and T. Saeki, Chem. Pharm. Bull. (Tokyo), 47, 903 (1999).
  4. J.Y. Lee, J.W. Yoon, C.T. Kim and S.T. Lim, Phytochemistry, 65, 3033 (2004).
  5. Y. Xie, Y.P. Ye, H.X. Sun and D. Li, Vaccine, 26, 3452 (2008).
  6. K.J. Lee, J.H. Choi, H.G. Kim, E.H. Han, Y.P. Hwang, Y.C. Lee, Y.C. Chung and H.G. Jeong, Food Chem. Toxicol., 46, 1778 (2008).
  7. E.H. Han, J.H. Park, J.Y. Kim, Y.C. Chung and H.G. Jeong, Food Chem. Toxicol., 47, 1069 (2009).
  8. K.J. Lee, S.J. Hwang, J.H. Choi and H.G. Jeong, Cancer Lett., 268, 233 (2008).
  9. J.Y. Kim, Y.P. Hwang, D.H. Kim, E.H. Han, Y.C. Chung, S.H. Roh and H.G. Jeong, Biosci. Biotechnol. Biochem., 70, 858 (2006).
  10. L.F. Han, M. Boakye-Yiadom, E.W. Liu, Y. Zhang, W. Li, X.B. Song, F.H. Fu and X.M. Gao, Phytochem. Anal., 23, 668 (2012).
  11. S.L. Man, W.Y. Gao, Y.J. Zhang, L.Q. Huang and C.X. Liu, Biomed. Chromatogr., 25, 712 (2011).
  12. L.P. Kang, K. Yu, Y. Zhao, Y.X. Liu, H.S. Yu, X. Pang, C.Q. Xiong, D.W. Tan, Y. Gao, C. Liu and B.P. Ma, J. Pharm. Biomed. Anal., 62, 235 (2012).
  13. W. Li, W. Zhang, L. Xiang, Z. Wang, Y.N. Zheng, Y.P. Wang, J. Zhang and L. Chen, Molecules, 15, 8702 (2010).
  14. H. Ishii, K. Tori, T. Tozyo and Y. Yoshimura, J. Chem. Soc. Perkin Trans. I, 661 (1984).
  15. Y.H. Choi, D.S. Yoo, M.R. Cha, C.W. Choi, Y.S. Kim, S.U. Choi, K.R. Lee and S.Y. Ryu, J. Nat. Prod., 73, 1863 (2010).
Read More
Author biographies is not available.
Download
Statistic
Read Counter : 0