1Key Laboratory of Natural Pharmaceutical and Chemical Biology of Yunnan Province, Honghe University, Mengzi 661199, P.R. China
2School of Science, Honghe University, Mengzi 661199, P.R. China
*Corresponding author: Tel/Fax: +86 873 3694923; E-mail: juchengzhang@163.com
He-Ping Yan1
1Key Laboratory of Natural Pharmaceutical and Chemical Biology of Yunnan Province, Honghe University, Mengzi 661199, P.R. China
2School of Science, Honghe University, Mengzi 661199, P.R. China
*Corresponding author: Tel/Fax: +86 873 3694923; E-mail: juchengzhang@163.com
Guo-Wei Zhang1
1Key Laboratory of Natural Pharmaceutical and Chemical Biology of Yunnan Province, Honghe University, Mengzi 661199, P.R. China
2School of Science, Honghe University, Mengzi 661199, P.R. China
*Corresponding author: Tel/Fax: +86 873 3694923; E-mail: juchengzhang@163.com
Xianlan Chen1
1Key Laboratory of Natural Pharmaceutical and Chemical Biology of Yunnan Province, Honghe University, Mengzi 661199, P.R. China
2School of Science, Honghe University, Mengzi 661199, P.R. China
*Corresponding author: Tel/Fax: +86 873 3694923; E-mail: juchengzhang@163.com
Li Zhang2
1Key Laboratory of Natural Pharmaceutical and Chemical Biology of Yunnan Province, Honghe University, Mengzi 661199, P.R. China
2School of Science, Honghe University, Mengzi 661199, P.R. China
*Corresponding author: Tel/Fax: +86 873 3694923; E-mail: juchengzhang@163.com
Wei Liu1
1Key Laboratory of Natural Pharmaceutical and Chemical Biology of Yunnan Province, Honghe University, Mengzi 661199, P.R. China
2School of Science, Honghe University, Mengzi 661199, P.R. China
*Corresponding author: Tel/Fax: +86 873 3694923; E-mail: juchengzhang@163.com
The effects of Fe3+, Fe2+ and Cu2+ ions on type I collagen were investigated by UV-visible and fluorescence spectra. The UV-visible spectra revealed that the Fe3+ ion can cause the 270 nm absorbance peak of type I collagen decreased, but Fe2+ and Cu2+ enhanced this peak. The fluorescence emission spectrum of the type I collagen show two emission band centered at 307/278 nm and 380/295 nm, adding Fe3+ will enhanced the intensity of emission band both 307 and 380 nm. However, adding Fe2+ and Cu2+ ions can not cause the two fluorescence peak enhanced evidently. The 380 nm fluorescence peak increased in presence of Fe3+ suggested the Fe3+ could induce pyridinoline crosslink formed in collagen solution.
Zhang1, J.-C.; Yan1, H.-P.; Zhang1, G.-W.; Chen1, X.; Zhang2, L.; Liu1, W. Effect of Fe2+ 3+ and Cu2+ Ions on Spectral Properties of Type I Collagen. ajc2013, 25, 10569-10571.
