1Department of Chemistry and Materials, College of Science, The Naval University of Engineering, Wuhan 430022, Hubei, P.R. China
2epartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, P.R. China
*Corresponding author: Fax: +86 27 83443066; Tel: +86 27 68771774, E-mail: chancellery11@gmail.com
Sen Li1
1Department of Chemistry and Materials, College of Science, The Naval University of Engineering, Wuhan 430022, Hubei, P.R. China
2epartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, P.R. China
*Corresponding author: Fax: +86 27 83443066; Tel: +86 27 68771774, E-mail: chancellery11@gmail.com
Ge-Qing Xia2
1Department of Chemistry and Materials, College of Science, The Naval University of Engineering, Wuhan 430022, Hubei, P.R. China
2epartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, P.R. China
*Corresponding author: Fax: +86 27 83443066; Tel: +86 27 68771774, E-mail: chancellery11@gmail.com
Yuansheng Wang1
1Department of Chemistry and Materials, College of Science, The Naval University of Engineering, Wuhan 430022, Hubei, P.R. China
2epartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, P.R. China
*Corresponding author: Fax: +86 27 83443066; Tel: +86 27 68771774, E-mail: chancellery11@gmail.com
The hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme is assembled on gold electrodes. It reveals bioelectrocatalytic properties and electrocatalyzes the reduction of H2O2. The bioelectrocatalytic functions of the hemin/G-quadruplex DNAzyme is used to develop electrochemical sensor for the detection of adenosine. Hairpin nucleic structure that includes the G-quadruplex sequence in a caged configuration and the nucleic acid sequence complementary to the aptamer sequence for adenosine, is immobilized on gold-electrode surfaces. In the presence of the analyte, the hairpin structure is opened and the hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme structure is generated on the electrode surfaces. The bioelectrocatalytic cathodic current generated by the functionalized electrode, upon the electrochemical reduction of H2O2, provides a way for the detection of adenosine.
Li1, Y., Li1, S., Xia2, G.-Q., & Wang1, Y. (2013). Label Free Detection of Adenosine Based on Hemin/G-Quadruplex Peroxidase-Mimicking DNAzyme as Bioelectrocatalyst. Asian Journal of Chemistry, 25(18), 10541–10545. https://doi.org/10.14233/ajchem.2013.15847
