Sensitive Analysis of Azelnidipine and Related Derivative in Human Plasma by Ultra-Performance Liquid Chromatography-Tandam Mass Spectrometry

G. Suneetha1; P. Venkateswarlu1; P.S.S. Prasad2

doi:10.14233/ajchem.2013.15290

Issue

Vol. 25 No. 18 (2013)

Issue Published : 2 December 2013

Sensitive Analysis of Azelnidipine and Related Derivative in Human Plasma by Ultra-Performance Liquid Chromatography-Tandam Mass Spectrometry

https://doi.org/10.14233/ajchem.2013.15290

G. Suneetha1

1Department of Chemistry, Sri Venkateswara University, Tirupati-517 502, India 2Department of Drugs Control Administration, Hyderabad-500 038, India *Corresponding author: E-mail: sunithareddy04@gmail.com

P. Venkateswarlu1

P.S.S. Prasad2

Corresponding Author(s) : G. Suneetha1

Asian Journal of Chemistry, Vol. 25 No. 18 (2013)
Article Published : 13 December 2013

Abstract

A rapid and sensitive method for analysis of azelnidipine in human plasma by ultra-performance liquid chromatography tandem mass spectrometry is presented. After pretreatment of a plasma sample by solid-phase extraction, azelnidipine was analyzed by the system with a C₁₈ column. This method gave satisfactory recovery rates, reproducibility and good linearity of calibration curve in the range of 0.01-10 ng/mL for quality control samples spiked with azelnidipine. The detection limit was as low as 1 pg/mL. This method seems very useful in forensic and clinical toxicology and pharmacokinetic studies.

Keywords

Azelnidipine Ethyl derivative of azelnidipine UPLC Human plasma.

(1)

Suneetha1, G.; Venkateswarlu1, P.; Prasad2, P. Sensitive Analysis of Azelnidipine and Related Derivative in Human Plasma by Ultra-Performance Liquid Chromatography-Tandam Mass Spectrometry. ajc 2013, 25, 10319-10321.