Determination of 2-Keto-L-gulonic Acid in Fermented Broth by Flow Injection Chemiluminescence Using Potassium Permanganate-Rhodamine B System

Xinli Lu1; Jing Feng2; Jujuan Zhang3; Suliang Chen1; Yuqi Zhang1; Hongru Zhao1

doi:10.14233/ajchem.2013.15220

Issue

Vol. 25 No. 18 (2013)

Issue Published : 2 December 2013

Determination of 2-Keto-L-gulonic Acid in Fermented Broth by Flow Injection Chemiluminescence Using Potassium Permanganate-Rhodamine B System

https://doi.org/10.14233/ajchem.2013.15220

Xinli Lu1

1Institute of STD/AIDS Prevention and Control, Hebei Center for Desease Prevention and Control, Shijiazhuang 050021, P.R. China 2Hebei College of Chemical & Pharmaceutical Vocational Technology, Shijiazhuang 050026, P.R. China 3Department of Finance and Economics, Shijiazhuang Vocational College of Finance and Economics, Shijiazhuang 050051, P.R. China *Corresponding author: E-mail: lxlii2009@163.com

Jing Feng2

Jujuan Zhang3

Suliang Chen1

Yuqi Zhang1

Hongru Zhao1

Corresponding Author(s) : Xinli Lu1

Asian Journal of Chemistry, Vol. 25 No. 18 (2013)
Article Published : 12 December 2013

Abstract

In this paper, a flow injection-chemiluminescence (FI-CL) was presented for the determination of 2-keto-L-gulonic acid in fermented broth. This method was based on enhance effect of 2-keto-L-gulonic acid on the chemiluminescence reaction between rhodamine B and potassium permanganate in acid medium. The optimized experimental conditions were evaluated. Under optimum conditions, calibration curve over the range of 0.2-60 mg L^-1 was obtained. The detection limit of this method was 0.04 mg L^-1. The relative standard deviation was 3.7 % for 2.0 mg L^-1 2-keto-L-gulonic acid. The method validation has been compared versus HPLC method for determination of 2-keto-L-gulonic acid in fermented broth.

Keywords

2-Keto-L-gulonic acid Flow injection Chemiluminescence Potassium permanganate Rhodamine B.

(1)

Lu1, X.; Feng2, J.; Zhang3, J.; Chen1, S.; Zhang1, Y.; Zhao1, H. Determination of 2-Keto-L-Gulonic Acid in Fermented Broth by Flow Injection Chemiluminescence Using Potassium Permanganate-Rhodamine B System. ajc 2013, 25, 10179-10181.