1Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, P.R. China
2School of Chemical Engineering & Technology, China University of Mining and Technology, Xuzhou 221116, P.R. China
3College of Environment and Spatial Informatics, China University of Mining and Technology, Xuzhou 221116, P.R. China
*Corresponding author: E-mail: mhmwbb@163.com
Hui-Min Ma1
1Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, P.R. China
2School of Chemical Engineering & Technology, China University of Mining and Technology, Xuzhou 221116, P.R. China
3College of Environment and Spatial Informatics, China University of Mining and Technology, Xuzhou 221116, P.R. China
*Corresponding author: E-mail: mhmwbb@163.com
Cai-Yan Zhu1
1Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, P.R. China
2School of Chemical Engineering & Technology, China University of Mining and Technology, Xuzhou 221116, P.R. China
3College of Environment and Spatial Informatics, China University of Mining and Technology, Xuzhou 221116, P.R. China
*Corresponding author: E-mail: mhmwbb@163.com
Jia-Jia Wang1
1Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, P.R. China
2School of Chemical Engineering & Technology, China University of Mining and Technology, Xuzhou 221116, P.R. China
3College of Environment and Spatial Informatics, China University of Mining and Technology, Xuzhou 221116, P.R. China
*Corresponding author: E-mail: mhmwbb@163.com
Bing-Bing Wang3
1Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, P.R. China
2School of Chemical Engineering & Technology, China University of Mining and Technology, Xuzhou 221116, P.R. China
3College of Environment and Spatial Informatics, China University of Mining and Technology, Xuzhou 221116, P.R. China
*Corresponding author: E-mail: mhmwbb@163.com
The novel coumarin derivative based quaternary ammonium salt had been designed and synthesized in two steps starting from 5-chloromethylsalicylaldehyde. 5-Chloromethylsalicylaldehyde first reacted with tertiary amine to yield the quaternary ammonium salt, then this compound reacted with the diethyl malonate on the appropriate condition to yield the corresponding coumarin derivative. The chemical structure had been elucidated by infrared spectra, 1H NMR spectra, LC-MS and melting point analysis. The novel coumarin had been evaluated for its antibacterial activities in vitro against foru gram-negative bacteria (Edwardsiella tarda, Aeromonas hydrophila, Vibrio anguillarum and Vibrio harveyi). The bioactive assay showed that the title compound displayed comparable antibacterial efficacy in comparison with some of other reference drugs.
