1Biochemical Engineering College of Beijing Union University, Beijing 100023, P.R. China
2Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China
3Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, P.R. China
*Corresponding author: Tel: +86 10 52072259; E-mail: huo_q2002@yahoo.com.cn
Jianan Liu2
1Biochemical Engineering College of Beijing Union University, Beijing 100023, P.R. China
2Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China
3Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, P.R. China
*Corresponding author: Tel: +86 10 52072259; E-mail: huo_q2002@yahoo.com.cn
Shaoxiang Xiong2
1Biochemical Engineering College of Beijing Union University, Beijing 100023, P.R. China
2Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China
3Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, P.R. China
*Corresponding author: Tel: +86 10 52072259; E-mail: huo_q2002@yahoo.com.cn
Rongjian Shi3
1Biochemical Engineering College of Beijing Union University, Beijing 100023, P.R. China
2Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China
3Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, P.R. China
*Corresponding author: Tel: +86 10 52072259; E-mail: huo_q2002@yahoo.com.cn
Matrix assisted laser desorption ionization time of flight mass spectrometry MALDI-TOF-MS was generally used to analyze macromolecular compounds. In this paper we study how to use the MALDI to determine the molecular weight of small molecule compounds, such as sugars, amino acids, clenbuterol, diphenylmethyl amines, plant hormones, etc. We also studied how to exploring the sensitivity of matrix and how to application the sensitivity. We find a macromolecular 1,2,3,4-tetrakis(3’,4’-hydroxyphenyl)-thiophene as matrix, it not only absorbs the laser energy but also makes sample desorption ionization. At the same time it does not produce interfering ions to low molecular weight. In addition, perfluoro C60 was added in matrix sensitivity can be improved.
Huo1, Q., Liu2, J., Xiong2, S., & Shi3, R. (2013). Identification of Small Biomolecular Compounds by MALDI-TOF-Mass Spectrometry. Asian Journal of Chemistry, 25(18), 10137–10143. https://doi.org/10.14233/ajchem.2013.15189
