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Reverse Phase High Performance Liquid Chromatographic Method for Separation of R-Rasagiline Mesylate from S-Isomer
Corresponding Author(s) : Ramakrishna Nirogi
Asian Journal of Chemistry,
Vol. 27 No. 1 (2015): Vol 27 Issue 1
Abstract
High-performance liquid chromatographic method has been developed under reverse-phase conditions for the separation of enantiomeric R-rasagiline mesylate (chemically known as (R)-N-(prop-2-ynyl)-2,3-dihydro-1H-inden-1-amine mesylate) from its S-isomer. Chromatographic separation was carried out on amylase based CHIRALPAK AD-RH (150 × 4.6 mm, 5 μm) column using a mobile phase consisting of 20 mM potassium dihydrogen phosphate in water-acetonitrile (65:35, v/v) adjusted to pH 6.9 with 10 % potassium hydroxide solution. Flow rate was 0.5 mL min-1 and UV detection was at 210 nm. The conditions affording the best resolution (3.4) were found by selection and variation of the mobile-phase composition and the difference in separation capability of the method is noted. Relative standard deviation of retention time and peak areas were found to be 0.03 and 0.23, respectively for precision. R-rasagiline mesylate sample solution and mobile phase were found to be stable for 72 h.
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References
V. Oldfield, G.M. Keating and C.M. Perry, Drugs, 67, 1725 (2007); doi:10.2165/00003495-200767120-00006.
G. Bertram Katzung, Basic and Clinical Pharmacology, Lange Medical Books - McGraw Hill Publishers, edn 9, pp. 453 (2004).
J.J. Chen, D.M. Swope and K. Dashtipour, Clin. Ther., 29, 1825 (2007); doi:10.1016/j.clinthera.2007.09.021.
R. Narendrakumar, G. Nageswara Rao and P.Y. Naidu, Int. J. Appl. Biol. Pharmt. Technol., 1, 247 (2010).
A.F. Shaughnessy, Pharmacotherapy, 24, 295 (2004); doi:10.1592/phco.24.2.295.33146.
M. Vijaya Lakshmi, J.V.L.N. Seshagiri Rao and A. Lakshmana Rao, Rayasayan J. Chem., 3, 621 (2010).
K.R. Jayavarapu, J. Murugeasn and P.K. Mantada, J. Pharm. Res., 4, 1376 (2011).
M. Song, L. Wang, H. Zhao, T. Hang, A. Wen, L. Yang and L. Jia, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 875, 515 (2008); doi:10.1016/j.jchromb.2008.10.005.
J. Ma, X. Chen, X. Duan, P. Deng, H. Wang and D. Zhong, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 873, 203 (2008); doi:10.1016/j.jchromb.2008.08.024.
G. Devalarao, S. Kathirvel and S.V. Satyanarayana, J. Pharm. Res., 4, 61 (2011).
B. Rama and K. Preeti, Int. J. Pharm. Sci. Rev. Res., 5, 5 (2010).
A.B. Binda, F. Hubalek, M. Li, Y. Herzig, J. Sterling, D.E. Edmondson and A. Mattevi, J. Med. Chem., 48, 8148 (2005); doi:10.1021/jm0506266.
K. Tatendra Reddy, K. Suneel Kumar, G. Omprakash and P.K. Dubey, Der Pharma Chemica, 3, 110 (2011).