Evaluation of Phenolic Compounds and Development of Chromatographic Profiles in Spathodea campanulata Inflorescence by HPTLC

Jyoti V. Vastrad; Giridhar Goudar

doi:10.14233/ajchem.2016.19370

Issue

Vol. 28 No. 3 (2016): Vol 28 Issue 3

Issue Published : December 5, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Evaluation of Phenolic Compounds and Development of Chromatographic Profiles in Spathodea campanulata Inflorescence by HPTLC

https://doi.org/10.14233/ajchem.2016.19370

Jyoti V. Vastrad

DBT Project, All India Co-ordinated Research Project on Home Science (Clothing and Textile) Department, University of Agricultural Sciences, Dharwad-580 005, India

Giridhar Goudar

DBT Project, All India Co-ordinated Research Project on Home Science (Clothing and Textile) Department, University of Agricultural Sciences, Dharwad-580 005, India

Corresponding Author(s) : Jyoti V. Vastrad

jyotivastrad@gmail.com

Asian Journal of Chemistry, Vol. 28 No. 3 (2016): Vol 28 Issue 3
Article Published : December 5, 2015

Abstract

In the present work, an investigation of phenolic constituents in flower petals of Spathodea campanulata was carried out. Ethyl alcohol, 50 % v/v ethyl alcohol and distilled water were used as solvents for the optimization of extraction process. The extracts obtained were subjected to phytochemical screening and quantified for total phenolic content and total flavonoid content. Total phenolic content of the extracts obtained were in the sequence: distilled water (25.75) > 50 % ethyl alcohol (15.12) > ethyl alcohol (2.37), which was expressed in terms of mg/g gallic acid equivalent (GAE) of sample. Whereas total flavonoid content of the extracts were in the sequence: distilled water (17.65) > 50 % ethyl alcohol (10.25) > ethyl alcohol (2.90) which was expressed in terms of mg/g rutin equivalent (RE) of sample. High performance thin layer chromatography analysis of the sample extracts was performed to obtain chromatographic profiles, scanning was performed at 270, 320 and 370 nm and the R_f values obtained for the sample extracts were compared with the R_f values of the flavonoid compounds viz., rutin, catechin and quercetin.

Keywords

Phytochemical HPTLC Spathodea campanulata Flavonoid

Vastrad, J. V., & Goudar, G. (2015). Evaluation of Phenolic Compounds and Development of Chromatographic Profiles in Spathodea campanulata Inflorescence by HPTLC. Asian Journal of Chemistry, 28(3), 497–500. https://doi.org/10.14233/ajchem.2016.19370

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References

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References

F. Delgado-Vargas, A.R. Jiménez and O. Paredes-López, Crit. Rev. Food Sci. Nutr., 40, 173 (2000); doi:10.1080/10408690091189257.

G. Kaur, M.S. Alam, Z. Jabbar, K. Javed and M. Athar, J. Ethnopharmacol., 108, 340 (2006); doi:10.1016/j.jep.2006.05.021.

Z. Youwei, Z. Jinlian and P. Yonghong, LWT–Food Sci. Technol., 41, 1586 (2008); doi:10.1016/j.lwt.2007.10.010.

J. Dai and R.J. Mumper, Molecules, 15, 7313 (2010); doi:10.3390/molecules15107313.

Z. Sroka and W. Cisowski, Food Chem. Toxicol., 41, 753 (2003); doi:10.1016/S0278-6915(02)00329-0.

R.E.K. Koes, F. Quattrocchio and J.N.M. Mol, BioEssays, 16, 123 (1994); doi:10.1002/bies.950160209.

J. Robak and R. Gryglewski, Pol. J. Pharmacol., 48, 555 (1996).

J.B. Harborne and C.A. Williams, Phytochemistry, 55, 481 (2000); doi:10.1016/S0031-9422(00)00235-1.

G. Di Carlo, N. Mascolo, A.A. Izzo and F. Capasso, Life Sci., 65, 337 (1999); doi:10.1016/S0024-3205(99)00120-4.

R. Croteau, T.M. Kutchan and N.G. Lewis, in eds.: B. Buchanan, W. Gruissem and R. Jones, Natural Products (Secondary Metabolites), In: Biochemistry and Molecular Biology of Plants, American Society of Plant Physiologists, Rockville, MD, p. 1250 (2000).

P.M. Dewick, Medicinal Natural Products: A Biosynthetic Approach. In: The Shikimate Pathway: Aromatic Amino Acids and Phenylpropanoids, Wiley, Chichester, edn 2, p. 149 (2002).

A.K. Samanta and A. Konar, in ed.: . In: Emriye Akcakoca Kumbasar, Dyeing of Textiles with Natural Dyes, In: Natural Dyes, Intech Publisher, Croatia, Europe, p. 29 (2011).

P. Guinot, A. Roge, A. Gargadennec, M. Garcia, D. Dupont, E. Lecoeur, L. Candelier and C. Andary, Colouration Technol., 122, 93 (2006); doi:10.1111/j.1478-4408.2006.00015.x.

R. Blanc, T. Espejo, A. López-Montes, D. Torres, G. Crovetto, A. Navalón and J.L. Vílchez, J. Chromatogr. A, 1122, 105 (2006); doi:10.1016/j.chroma.2006.04.056.

B. Szostek, J. Orska-Gawrys, I. Surowiec and M. Trojanowicz, J. Chromatogr. A, 1012, 179 (2003); doi:10.1016/S0021-9673(03)01170-1.

V.L. Suryavanshi, P.A. Sathe, M.M. Baing, G.R. Singh and S.N. Lakshmi, Chromatographia, 65, 767 (2007); doi:10.1365/s10337-007-0239-1.

Š. Sona, M. Ladislava and M. Ludmila, Advances in Food and Nutrition Research, Oxford University Press, New York, Vol. 67, Chap. 3, p. 75 (2012).

R. Chulet, P. Pradhan, S.K. Sharma, M.K. Jhajharia, J. Chem. Pharm. Res., 2, 476 (2010).

I.A. Ajayi, O. Ajibade and R.A. Oderinde, Res. J. Chem. Sci., 1, 58 (2011).

V.L. Singleton and J.A. Rossi, Am. J. Enol. Vitic., 16, 144 (1965).

J.V. Vastrad, S.A. Byadgi, G. Goudar and R. Kotur, Forest Products J., 64, 259 (2014); doi:10.13073/FPJ-D-13-00049.

Y. Shen, L. Jin, P. Xiao, Y. Lu and J. Bao, J. Cereal Sci., 49, 106 (2009); doi:10.1016/j.jcs.2008.07.010.

Issue

Vol. 28 No. 3 (2016): Vol 28 Issue 3

Evaluation of Phenolic Compounds and Development of Chromatographic Profiles in Spathodea campanulata Inflorescence by HPTLC

Corresponding Author(s) : Jyoti V. Vastrad

Abstract

Keywords

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