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Vol. 28 No. 3 (2016): Vol 28 Issue 3

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Optimization of Various Parameters for Maximum Production of Glucoamylaze by Neurospora sitophila

https://doi.org/10.14233/ajchem.2016.19462
Fatima Syed
Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan
M. Javaid Asad
Department of Biochemistry, PMAS, Arid Agriculture University, Rawalpindi, Pakistan
Khurshid Ali
Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan
Muhammad Gulfraz
Department of Biochemistry, PMAS, Arid Agriculture University, Rawalpindi, Pakistan
Aftab Ahmad
Beijing University of Chemical Technology, Beijing, P.R. China
Zahid Khan
Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan
Muhammad Imran
Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan
Raheela Taj
Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan
Shafiulah Khan
Institute of Chemical Sciences, Gomal University, D.I. Khan, Pakistan

Corresponding Author(s) : Fatima Syed

fatimasyed_chem81@yahoo.com

Asian Journal of Chemistry, Vol. 28 No. 3 (2016): Vol 28 Issue 3

Abstract

Glucoamylaze production by Neurospora sitophila was investigated under solid state fermentation. A number of process parameters including moisture content (%), pH, incubation temperature (°C), inoculum size (%), nutrients and duration of fermentation (h) were optimized using response surface methodology. The statistical results showed that the best culture conditions for glucoamylaze production were pH 5, temperature 37.5 °C and 72 h of incubation period while the optimum medium conditions were 15 g wheat bran, moisture content 65 %, inoculum level 20 %, starch 1.17 %, NH4NO3 0.66 % and SDS 0.3 % of the substrate weight. Under these optimized conditions, the enzyme production was enhanced to 335.2 ± 12 U/gds which is approximately 2.6-fold increase in the enzyme production when compared with the initial fermentation conditions. To our best of knowledge, this is the first report on optimization of process variables using response surface methodology for glucoamylaze production under solid state fermentation from Neurospora sitophila.

Keywords

Glucoamylaze Neurospora sitophila Response surface methodology Optimization
Syed, F., Asad, M. J., Ali, K., Gulfraz, M., Ahmad, A., Khan, Z., … Khan, S. (2015). Optimization of Various Parameters for Maximum Production of Glucoamylaze by Neurospora sitophila. Asian Journal of Chemistry, 28(3), 669–674. https://doi.org/10.14233/ajchem.2016.19462
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