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Potential of Using High Rate Algal Pond for Algal Biofuel Production and Wastewater Treatment
Corresponding Author(s) : Gamila H. Ali
Asian Journal of Chemistry,
Vol. 28 No. 2 (2016): Vol 28 Issue 2
Abstract
The aim of this study is to investigate the application of high rate algal pond for municipal wastewater treatment and the production of microalgae biomass for biofuel production. Primary facultative pond one cubic meter (1 m3) followed by high rate algal pond (1.5 m3) were installed in wastewater treatment plant and fed with municipal wastewater. High rate algal pond enhance the removal of nutrients, organic contaminants, coliforms and E. coli. The performance data of the treatment system showed that the removal efficiency of COD, BOD, total suspended solids and ammonia were 31, 33, 22 and 56 %, respectively. The removal of total coliforms reached up to 3 logs. Furthermore, fecal coliforms and E. coli removal reached 3 and 5 logs, respectively. The community structure of the high rate algal pond revealed that the most dominant algal species were Scenedesmes obliquus and Micracitinium pusillum. The total lipid percentage was an average of 5 % in the high rate algal pond. The gas chromatographic analysis of the fatty acid methyl ester revealed that stearic acid (C18:0) concentration reached up to 18.8 %, the oleic acid and linolenic acid were detected with an average percentage of 10.3 % and 13.8 %, respectively. It can be concluded that, the high rate algal pond considered as an efficient and cost-effective pond system for municipal wastewater treatment. Also, it able to produced oil used as biodiesel source from the biomass of the harvested microalgae.
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- L. Brennan and P. Owende, Renew. Sustain. Energy Rev., 14, 557 (2010); doi:10.1016/j.rser.2009.10.009.
- A. Srivastava and R. Prasad, Renew. Sustain. Energy Rev., 4, 111 (2000); doi:10.1016/S1364-0321(99)00013-1.
- Y. Chisti, Biotechnol. Adv., 25, 294 (2007); doi:10.1016/j.biotechadv.2007.02.001.
- S. Shafiee and E. Topal, Energy Policy, 37, 181 (2009); doi:10.1016/j.enpol.2008.08.016.
- J.P.K. Park, R.J. Craggs and A. Shilton, Bioresour. Technol., 102, 35 (2011); doi:10.1016/j.biortech.2010.06.158.
- J. Sheehan, T. Dunahay, J. Benemann and P. Roessler, National Renewable Energy Laboratory, NREL/TP-580-24190 (1998).
- Y. Chisti, Trends Biotechnol., 26, 126 (2008); doi:10.1016/j.tibtech.2007.12.002.
- D.E. Brune, T.J. Lundquist and J.R. Benemann, J. Environ. Eng., 135, 1136 (2009); doi:10.1061/(ASCE)EE.1943-7870.0000100.
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- J.R. Benemann, Singapore, November 17–18, p. 15 (2008).
- M. Tampier, Suitability and Barriers to Implementation Prepared for the British Columbia Innovation Council. January 14 (2009).
- R.H. Wijffels, M.J. Barbosa and M.H.M. Eppink, Bioprod. Biorefining, 4, 287 (2010); doi:10.1002/bbb.215.
- A. Richmond, A. Vonshak and S.M. Arad, Elsevier/North Holland Biomedical Press, Amsterdam, The Netherlands, pp. 65–73 (1980).
- J.C. Weissman, R.P. Goebel and J.R. Benemann, Biotechnol. Bioeng., 31, 336 (1988); doi:10.1002/bit.260310409.
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- A. Abeliovich, Handbook of Microalgal Mass Culture, CRC Press, Boca Raton, FL, pp. 331-338 (1986).
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- W.J. Oswald, in eds.: C.A. Lembi and J.R. Waaland, The Role of Microalgae in Liquid Waste Treatment and Reclamation Algae and Human Affairs, Cambridge University Press, Cambridge, pp. 255–282 (1988).
- W.J. Oswald, Water Sci. Technol., 24, 1 (1991).
- J. García, R. Mujeriego and M. Hernández, Ingeniería del Agua, 5, 35 (1998).
- J.T. Komárek, B. Fott and G. Huber-Pestalozzi, Das Phytoplankton des Süsswassers, Die Binnengewässer 16, 7/1: 1-1044, Schweizerbart Verlag, Stuttgart, Deutschland (1983).
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- S.M. Abdo, M.H. Hetta, F.A. Samhan, R.A.S. El Din and G.H. Ali, Asian J. Plant Sci., 11, 109 (2012); doi:10.3923/ajps.2012.109.116.
- APHA, American Public Health Association, Standard Methods for the Examination of Water and Wastewater, Washington, D.C., edn 22 (2012).
- E.G. Bligh and W.J.A. Dyer, Can. J. Biochem. Physiol., 37, 911 (1959); doi:10.1139/o59-099.
- A. Metcalf and A. Eddy, Treatment and Reuse, McGraw-Hill. New, York. USA, edn 4 (2003).
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- W.H. Rössle and W.A. Pretorius, Water S.A., 27, 405 (2001).
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- G. Bitton, Florida Storm Water Association, pp. 1-71 (2005).
- M.A. El-Liethy, Ph.D. Thesis, Microbiology Department, Faculty of Science, Ain Shams University, Egypt (2013).
- Z. Paluszak, A. Ligocka and B. Breza-Boruta, Pol. J. Environ. Stud., 12, 345 (2003).
- P.K. Robison, Y.S. Wong and F.Y. Tamnora, Wastewater Treatment with Algae, Springer and Landes Bioscience, pp. 1-16 (1998).
- G. Schumacher, T. Blumem and I. Sekoulov, Water Sci. Technol., 47, 195 (2003).
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- USEPA, Status Report EPA-823-R-03-008, US Environmental Protection Agency, Washington D.C. (2003).
- WHO, Health Guidelines for the Use of Wastewater in Agriculture and Aquaculture, Geneva, Report No. 778 (1989).
- C.S. Reynolds, in eds.: M. Usher, D. Saunders, R. Peet and A. Dobson, Ecology of Phytoplankton, Cambridge University Press, Cambridge, p. 535 (2006)..
- D.M. Tillett, Ph.D. Thesis, The School of Chemical Engineering, Georgia Institute of Technology, USA (1988).
- O. Pulz, Appl. Microbiol. Biotechnol., 57, 287 (2001); doi:10.1007/s002530100702.
- S.M. Abdo, E. Ahmed, S.A. El-Enin, R.S. El-Din, G. El-Diwani and G.H. Ali, J. Algal Biomass Utln., 4, 51 (2013).
- K.E. Cooksey, J.B. Guckert, S.A. Williams and P.R. Callis, J. Microbiol. Methods, 6, 333 (1987); doi:10.1016/0167-7012(87)90019-4.
- M.J. Griffiths and S.T.L. Harrison, J. Appl. Phycol., 21, 493 (2009); doi:10.1007/s10811-008-9392-7.
References
L. Brennan and P. Owende, Renew. Sustain. Energy Rev., 14, 557 (2010); doi:10.1016/j.rser.2009.10.009.
A. Srivastava and R. Prasad, Renew. Sustain. Energy Rev., 4, 111 (2000); doi:10.1016/S1364-0321(99)00013-1.
Y. Chisti, Biotechnol. Adv., 25, 294 (2007); doi:10.1016/j.biotechadv.2007.02.001.
S. Shafiee and E. Topal, Energy Policy, 37, 181 (2009); doi:10.1016/j.enpol.2008.08.016.
J.P.K. Park, R.J. Craggs and A. Shilton, Bioresour. Technol., 102, 35 (2011); doi:10.1016/j.biortech.2010.06.158.
J. Sheehan, T. Dunahay, J. Benemann and P. Roessler, National Renewable Energy Laboratory, NREL/TP-580-24190 (1998).
Y. Chisti, Trends Biotechnol., 26, 126 (2008); doi:10.1016/j.tibtech.2007.12.002.
D.E. Brune, T.J. Lundquist and J.R. Benemann, J. Environ. Eng., 135, 1136 (2009); doi:10.1061/(ASCE)EE.1943-7870.0000100.
E. Stephens, I.L. Ross, Z. King, J.H. Mussgnug, O. Kruse, C. Posten, M.A. Borowitzka and B. Hankamer, Nat. Biotechnol., 28, 126 (2010); doi:10.1038/nbt0210-126.
G.K. Pittman, A.P. Dean and O. Osundeko, Bioresour. Technol., 102, 17 (2011); doi:10.1016/j.biortech.2010.06.035.
J.R. Benemann, Singapore, November 17–18, p. 15 (2008).
M. Tampier, Suitability and Barriers to Implementation Prepared for the British Columbia Innovation Council. January 14 (2009).
R.H. Wijffels, M.J. Barbosa and M.H.M. Eppink, Bioprod. Biorefining, 4, 287 (2010); doi:10.1002/bbb.215.
A. Richmond, A. Vonshak and S.M. Arad, Elsevier/North Holland Biomedical Press, Amsterdam, The Netherlands, pp. 65–73 (1980).
J.C. Weissman, R.P. Goebel and J.R. Benemann, Biotechnol. Bioeng., 31, 336 (1988); doi:10.1002/bit.260310409.
A. Richmond, J. Appl. Phycol., 4, 281 (1992); doi:10.1007/BF02161213.
S. Heubeck, R.J. Craggs and A. Shilton, Water Sci. Technol., 55, 193 (2007); doi:10.2166/wst.2007.358.
J.B.K. Park and R.J. Craggs, Water Sci. Technol., 61, 633 (2010); doi:10.2166/wst.2010.951.
W. Zhou, P. Chen, M. Min, X. Ma, J. Wang, R. Griffith, F. Hussain, P. Peng, Q. Xie, Y. Li, J. Shi, J. Meng and R. Ruan, Renew. Sustain. Energy Rev., 36, 256 (2014); doi:10.1016/j.rser.2014.04.073.
A. Abeliovich, Handbook of Microalgal Mass Culture, CRC Press, Boca Raton, FL, pp. 331-338 (1986).
D.E. Brune, G. Schwartz, A.G. Eversole, J.A. Collier and T.E. Schwedler, Aquacult. Eng., 28, 65 (2003); doi:10.1016/S0144-8609(03)00025-6.
W.J. Oswald, in eds.: C.A. Lembi and J.R. Waaland, The Role of Microalgae in Liquid Waste Treatment and Reclamation Algae and Human Affairs, Cambridge University Press, Cambridge, pp. 255–282 (1988).
W.J. Oswald, Water Sci. Technol., 24, 1 (1991).
J. García, R. Mujeriego and M. Hernández, Ingeniería del Agua, 5, 35 (1998).
J.T. Komárek, B. Fott and G. Huber-Pestalozzi, Das Phytoplankton des Süsswassers, Die Binnengewässer 16, 7/1: 1-1044, Schweizerbart Verlag, Stuttgart, Deutschland (1983).
J.T. Komárek and K. Anagnostidis, in eds.: H. Ettl, G. Gärtner, H. Heynigh and D. Mollenhauer, Süsswasserflora von Mitteleuropa, 19/1 Gustav Fischer, Jena-Stuttgart-Lübeck-Ulm, 548 pp. (1998).
S.M. Abdo, M.H. Hetta, F.A. Samhan, R.A.S. El Din and G.H. Ali, Asian J. Plant Sci., 11, 109 (2012); doi:10.3923/ajps.2012.109.116.
APHA, American Public Health Association, Standard Methods for the Examination of Water and Wastewater, Washington, D.C., edn 22 (2012).
E.G. Bligh and W.J.A. Dyer, Can. J. Biochem. Physiol., 37, 911 (1959); doi:10.1139/o59-099.
A. Metcalf and A. Eddy, Treatment and Reuse, McGraw-Hill. New, York. USA, edn 4 (2003).
R. Íleri, Environmental Biotechnology, Degisim Yayinlari, pp. 160 (2000)..
W.H. Rössle and W.A. Pretorius, Water S.A., 27, 405 (2001).
K. Chojnacka, A. Chojnacki and H. Górecka, Chemosphere, 59, 75 (2005); doi:10.1016/j.chemosphere.2004.10.005.
G. Bitton, Florida Storm Water Association, pp. 1-71 (2005).
M.A. El-Liethy, Ph.D. Thesis, Microbiology Department, Faculty of Science, Ain Shams University, Egypt (2013).
Z. Paluszak, A. Ligocka and B. Breza-Boruta, Pol. J. Environ. Stud., 12, 345 (2003).
P.K. Robison, Y.S. Wong and F.Y. Tamnora, Wastewater Treatment with Algae, Springer and Landes Bioscience, pp. 1-16 (1998).
G. Schumacher, T. Blumem and I. Sekoulov, Water Sci. Technol., 47, 195 (2003).
S. Elshafai, F. Elgohary, F. Nasr, N. Petervandersteen and H. Gijzen, Bioresour. Technol., 98, 798 (2007); doi:10.1016/j.biortech.2006.03.011.
R.J. Craggs, A. Zwart, J.W. Nagels and R.J. Davies-Colley, Ecol. Eng., 22, 113 (2004); doi:10.1016/j.ecoleng.2004.03.001.
USEPA, Status Report EPA-823-R-03-008, US Environmental Protection Agency, Washington D.C. (2003).
WHO, Health Guidelines for the Use of Wastewater in Agriculture and Aquaculture, Geneva, Report No. 778 (1989).
C.S. Reynolds, in eds.: M. Usher, D. Saunders, R. Peet and A. Dobson, Ecology of Phytoplankton, Cambridge University Press, Cambridge, p. 535 (2006)..
D.M. Tillett, Ph.D. Thesis, The School of Chemical Engineering, Georgia Institute of Technology, USA (1988).
O. Pulz, Appl. Microbiol. Biotechnol., 57, 287 (2001); doi:10.1007/s002530100702.
S.M. Abdo, E. Ahmed, S.A. El-Enin, R.S. El-Din, G. El-Diwani and G.H. Ali, J. Algal Biomass Utln., 4, 51 (2013).
K.E. Cooksey, J.B. Guckert, S.A. Williams and P.R. Callis, J. Microbiol. Methods, 6, 333 (1987); doi:10.1016/0167-7012(87)90019-4.
M.J. Griffiths and S.T.L. Harrison, J. Appl. Phycol., 21, 493 (2009); doi:10.1007/s10811-008-9392-7.