Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography

Nurul I. Rasli; Syed M. Saufi; M.N. Abu Seman

doi:10.14233/ajchem.2016.19377

Issue

Vol. 28 No. 2 (2016): Vol 28 Issue 2

Issue Published : November 3, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography

https://doi.org/10.14233/ajchem.2016.19377

Nurul I. Rasli

Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Kuantan, Pahang, Malaysia

Syed M. Saufi

Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Kuantan, Pahang, Malaysia

M.N. Abu Seman

Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Kuantan, Pahang, Malaysia

Corresponding Author(s) : Syed M. Saufi

smsaufi@gmail.com

Asian Journal of Chemistry, Vol. 28 No. 2 (2016): Vol 28 Issue 2
Article Published : November 3, 2015

Abstract

Membrane chromatography has been used widely as an alternative to the conventional packed bed chromatography for protein separation. Membrane chromatography used an adsorptive membrane that carried specific chromatography functionality. In the current study, a membrane chromatography was prepared by modification of commercial polyamide microfiltration membrane with acrylic acid monomer. Two modification methods were compared which are UV photo grafting and chemical grafting via redox reaction. Modification parameters studied were initiator concentration (1-50 mM), monomer concentration (0.2-5 M) and reaction time (5-60 min). The highest lysozyme binding capacity achieved was 0.175 mg of lysozyme/cm² membrane for the membrane prepared via UV photo grafting using 10 mM of photo-initiator, 0.1 M of acrylic acid and 15 min of reaction time.

Keywords

Protein separation Membrane chromatography UV grafting Chemical grafting Polyamide membrane

Rasli, N. I., Saufi, S. M., & Seman, M. A. (2015). Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography. Asian Journal of Chemistry, 28(2), 395–398. https://doi.org/10.14233/ajchem.2016.19377

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References

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References

T.M. Przybycien, N.S. Pujar and L.M. Steele, Curr. Opin. Biotechnol., 15, 469 (2004); doi:10.1016/j.copbio.2004.08.008.

R. Ghosh, J. Chromatogr. A, 952, 13 (2002); doi:10.1016/S0021-9673(02)00057-2.

E. Klein, J. Membr. Sci., 179, 1 (2000); doi:10.1016/S0376-7388(00)00514-7.

X. Zeng and E. Ruckenstein, Biotechnol. Prog., 15, 1003 (1999); doi:10.1021/bp990120e.

S.M. Saufi and C.J. Fee, Biotechnol. Bioeng., 103, 138 (2009); doi:10.1002/bit.22239.

J. Deng, L. Wang, L. Liu and W. Yang, Prog. Polym. Sci., 34, 156 (2009); doi:10.1016/j.progpolymsci.2008.06.002.

M. Taniguchi and G. Belfort, J. Membr. Sci., 231, 147 (2004); doi:10.1016/j.memsci.2003.11.013.

A.A. Abuhabib, A.W. Mohammad, N. Hilal, R.A. Rahman and A.H. Shafie, Desalination, 295, 16 (2012); doi:10.1016/j.desal.2012.03.020.

Y. Mansourpanah and E. Momeni Habili, J. Membr. Sci., 430, 158 (2013); doi:10.1016/j.memsci.2012.11.065.

B. Van der Bruggen, Eur. Polym. J., 45, 1873 (2009); doi:10.1016/j.eurpolymj.2009.04.017.

M. Wang, Q.-F. An, L.-G. Wu, J.-X. Mo and C.-J. Gao, J. Membr. Sci., 287, 257 (2007); doi:10.1016/j.memsci.2006.10.049.

S. Belfer, Y. Purinson, R. Fainshtein, Y. Radchenko and O. Kedem, J. Membr. Sci., 139, 175 (1998); doi:10.1016/S0376-7388(97)00248-2.

R. Bernstein, S. Belfer and V. Freger, Langmuir, 26, 12358 (2010); doi:10.1021/la1017278.

M. Ulbricht and H. Yang, Chem. Mater., 17, 2622 (2005); doi:10.1021/cm0485714.

S. Belfer, R. Fainchtain, Y. Purinson and O. Kedem, J. Membr. Sci., 172, 113 (2000); doi:10.1016/S0376-7388(00)00316-1.

S. Schwark and M. Ulbricht, Eur. Polym. J., 48, 1914 (2012); doi:10.1016/j.eurpolymj.2012.08.003.

D. He and M. Ulbricht, J. Membr. Sci., 315, 155 (2008); doi:10.1016/j.memsci.2008.02.014.

L.B. Bai, C.Y. Wang, J. Jin and Y.H. Liu, Iran. Polym. J., 18, 847 (2009).

A. Yusof and M. Ulbricht, Desalination, 236, 16 (2009); doi:10.1016/j.desal.2007.10.045.

J. Meng, J. Li, Y. Zhang and S. Ma, J. Membr. Sci., 455, 405 (2014); doi:10.1016/j.memsci.2014.01.007.

I. Kaur, N. Sharma and V. Kumari, J. Adv. Res., 4, 547 (2013); doi:10.1016/j.jare.2012.11.003.

K. Littunen, U. Hippi, L.-S. Johansson, M. Österberg, T. Tammelin, J. Laine and J. Seppälä, Carbohydr. Polym., 84, 1039 (2011); doi:10.1016/j.carbpol.2010.12.064.

O.S. Kushwaha, C.V. Avadhani and R.P. Singh, Adv. Mater. Lett., 5, 272 (2014); doi:10.5185/amlett.2014.10533.

N. Anuraj, S. Bhattacharjee, J.H. Geiger, G.L. Baker and M.L. Bruening, J. Membr. Sci., 389, 117 (2012); doi:10.1016/j.memsci.2011.10.022.

M. Homayoonfal, A. Akbari and M. Mehrnia, Desalination, 263, 217 (2010); doi:10.1016/j.desal.2010.06.062.

Issue

Vol. 28 No. 2 (2016): Vol 28 Issue 2

Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography

Corresponding Author(s) : Syed M. Saufi

Abstract

Keywords

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