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Vol. 28 No. 11 (2016): Vol 28 Issue 11

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Zwitterionic Ion Chromatography of Dansyl Amino Acids with 4-Vinylbenzyl Dimethyl Ammonio Pentanesulfonate as Stationary Phase

https://doi.org/10.14233/ajchem.2016.19993
Bashaer Abbas Al-Phalahy
Department of Chemistry, College of Science, University of Al-Nahrain, Baghdad, Iraq
Yasmeen H. Muhamad
Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq
Ashraf Saad Rasheed
Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq

Corresponding Author(s) : Bashaer Abbas Al-Phalahy

bashaer_abbas@yahoo.com

Asian Journal of Chemistry, Vol. 28 No. 11 (2016): Vol 28 Issue 11

Abstract

A high-capacity (488 μeq g–1) zwitterionic stationary phase was synthesized by attachment of zwitterionic precursor to polystyrene-divinylbenzene particles by graft reaction. Zwitterionic ion chromatography for the determination of dansyl-L-asparagine (D-L-Asp), dansyl-glycine (D-L-Gly), dansyl-L-valine (D-L-Val) and dansyl-L-leucine (D-L-Leu) was developed. Chromatographic characteristics of the stationary phase are investigated by separation of dansyl-amino acids using buffer eluent with UV/visible detection. It could be demonstrated that dansyl amino acids separation on zwitterionic stationary phase are mainly driven by a cation exchange mechanism. The sulfobetaine column already mentioned exhibited the difference as compared to the commercial columns.

Keywords

Amino acid Ion exchange Zwitterionic stationary phase Sulfobetaine Capacity
Al-Phalahy, B. A., Muhamad, Y. H., & Rasheed, A. S. (2016). Zwitterionic Ion Chromatography of Dansyl Amino Acids with 4-Vinylbenzyl Dimethyl Ammonio Pentanesulfonate as Stationary Phase. Asian Journal of Chemistry, 28(11), 2411–2414. https://doi.org/10.14233/ajchem.2016.19993
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