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A Rapid and Non-Thermal Process of Five Flavonoids from Safflower using Ultrahigh Pressure-Assisted Extraction

https://doi.org/10.14233/ajchem.2015.16660
Feng Liu
Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, Shandong Province, P.R. China
Li Fan
Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, Shandong Province, P.R. China
Jia Li
ShanCollege of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong Province, P.R. China
Yongde Li
Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, Shandong Province, P.R. China
Wenjuan Duan
Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, Shandong Province, P.R. China
Xiao Wang
Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, Shandong Province, P.R. China
Hongjun Yang
Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing 100700, Beijing Province, P.R. China

Corresponding Author(s) : Xiao Wang

wxjn1998@126.com

Asian Journal of Chemistry, Vol. 27 No. 1 (2015): Vol 27 Issue 1

Abstract

A rapid and non-thermal process using ultrahigh pressure extraction has been applied in extracting five flavonoids from safflower. The optimized ultrahigh pressure extraction conditions of the target compounds was 40 % ethanolic solution (v/v) as extraction solvent, 100 MPa of extraction pressure, 1 min of extraction time, 1:30 (g/mL) of solid/liquid ratio and only 1 cycle. Compared with HRE and ultrahigh pressure extraction, the ultrahigh pressure extraction technique showed highest efficiency in extracting target components. The yields of hydroxysafflor yellow A, rutin, safflor yellow A, astragalin and kaempferol were 31.59, 0.23, 11.86, 1.31 and 0.080 mg/g, respectively. The contents of five flavonoids in seven safflowers from different regions were analyzed by the ultrahigh pressure extraction-HPLC method. The samples with highest total content of five flavonoids originated from Xinjiang, China. In addition, SEM micrographs revealed that the florets tissues were disrupted after ultrahigh pressure extraction process in the optimized conditions.

Keywords

Safflower Flavonoids Ultrahigh pressure extraction
Liu, F., Fan, L., Li, J., Li, Y., Duan, W., Wang, X., & Yang, H. (2014). A Rapid and Non-Thermal Process of Five Flavonoids from Safflower using Ultrahigh Pressure-Assisted Extraction. Asian Journal of Chemistry, 27(1), 33–38. https://doi.org/10.14233/ajchem.2015.16660
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