Separation/Preconcentration and Determination of Scutellarin in Urine by Vortex-Assisted Surfactant-Enhanced Emulsification Microextraction and High Performance Liquid Chromatography

Jinyan Ning; Chao Yang; Li Bi; Jiao Zhao; Lifen Meng; Yaling Yang

doi:10.14233/ajchem.2015.16668

Issue

Vol. 27 No. 1 (2015): Vol 27 Issue 1

Issue Published : December 27, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Separation/Preconcentration and Determination of Scutellarin in Urine by Vortex-Assisted Surfactant-Enhanced Emulsification Microextraction and High Performance Liquid Chromatography

https://doi.org/10.14233/ajchem.2015.16668

Jinyan Ning

Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province 650500, P.R. China

Chao Yang

Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province 650500, P.R. China; Beijing Institute of Medical Device Testing, Beijing 100011, P.R. China

Li Bi

College of Science, Kunming University of Science and Technology, Yunnan Province 650500, P.R. China

Jiao Zhao

Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province 650500, P.R. China

Lifen Meng

Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province 650500, P.R. China

Yaling Yang

Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan Province 650500, P.R. China

Corresponding Author(s) : Yaling Yang

yilyil8@163.com

Asian Journal of Chemistry, Vol. 27 No. 1 (2015): Vol 27 Issue 1
Article Published : December 26, 2014

Abstract

Vortex-assisted surfactant-enhanced emulsification microextraction and high performance liquid chromatography (VASEME-HPLC) for the preconcentration and determination of trace amounts of scutellarin in urine was developed. Pentanol and Triton X-100 were used as the extraction solvent and emulsifier, respectively. The factors influencing the extraction, such as the kind and volume of the extraction solvent and emulsifier, pH were optimized. Under the optimum conditions, the linear dynamic range was between 0.04-24 μg/mL (r = 0.9977). The limit of detection (LOD, S/N = 3) was 1.3 ng/mL. The limit of quantification (LOQ, S/N = 10) was 3.9 ng/mL. The relative standard deviation (RSD) at 0.04 μg/mL level of scutellarin (n = 5) was found to be 3.1 %. Good recovery results (86.3-93.6 %) were obtained when the proposed method was applied to determine scutellarin in urine samples.

Keywords

Surfactant Emulsification Microextraction Scutellarin High performance liquid chromatography

Ning, J., Yang, C., Bi, L., Zhao, J., Meng, L., & Yang, Y. (2014). Separation/Preconcentration and Determination of Scutellarin in Urine by Vortex-Assisted Surfactant-Enhanced Emulsification Microextraction and High Performance Liquid Chromatography. Asian Journal of Chemistry, 27(1), 43–46. https://doi.org/10.14233/ajchem.2015.16668

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References

W.D. Zhang, W.S. Chen, Y.H. Wang, W.Y. Liu, D.Y. Kong and H.T. Li, China J. Chinese Mater. Med., 25, 536 (2000).
Z.J. Dai, W.F. Lu, J. Gao, H.-F. Kang, Y.-G. Ma, S.-Q. Zhang, Y. Diao, S. Lin, X.-J. Wang and W.-Y. Wu, BMC Complement. Altern. Med., 13, 150 (2013); doi:10.1186/1472-6882-13-150.
L.L. Lin, A.J. Liu, J.G. Liu, X.-H. Yu, L.-P. Qin and D.-F. Su, J. Cardiovasc. Pharmacol., 50, 327 (2007); doi:10.1097/FJC.0b013e3180cbd0e7.
H.F. Zhang, X.M. Hu, L.X. Wang, S.-Q. Xu and F.-D. Zeng, Planta Med., 75, 121 (2009); doi:10.1055/s-0028-1088368.
C. Gao, J. Liu, Y. Jia, L.-N. Xue, R.-L. Wang, D.-K. Li, F.-H. Jia and J.-M. Yan, China Pharmacy, 27, 2156 (2008).
R. Fang and S. Du, Chinese J. Exp. Tradit. Medical Formulae, 4, 077 (2011).
S.H. Li, X.H. Jiang, Q.J. Yang and Z.H. Jin, J. Biomed. Eng., 4, 033 (2003).
G.E.Q. Hua, Z. Zhou, X.J. Zhi, L.L. Ma and X.H. Chen, Chinese J. Pharmaceuticals, 12, (2003).
Y.H. Chao, X.M. Xu and J.N. Yu, Chinese Pharm. Affairs, 7, 032 (2010).
Y. Shao, Q.N. Ping, F. Cao and J.X. Guo, Chinese J. Natural Med., 3, (2007).
M. Gao, W. Huang, M. Roy Chowdhury and C. Liu, Anal. Chim. Acta, 591, 161 (2007); doi:10.1016/j.aca.2007.04.004.
X. Hao, G. Cheng, J. Sun, M. Zou, J. Yu, S. Zhang and F. Cui, J. Pharm. Biomed. Anal., 38, 360 (2005); doi:10.1016/j.jpba.2005.01.004.
S. Yulan and F. Fang, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 830, 1 (2006); doi:10.1016/j.jchromb.2005.10.016.
Y. Zhang, R. Gao, J.-X. Liu and S.-C. Wang, Chinese J. Clin. Pharmacol., 23, 299 (2007).
M. Gao, W. Huang and C.Z. Liu, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 858, 22 (2007); doi:10.1016/j.jchromb.2007.07.046.

References

W.D. Zhang, W.S. Chen, Y.H. Wang, W.Y. Liu, D.Y. Kong and H.T. Li, China J. Chinese Mater. Med., 25, 536 (2000).

Z.J. Dai, W.F. Lu, J. Gao, H.-F. Kang, Y.-G. Ma, S.-Q. Zhang, Y. Diao, S. Lin, X.-J. Wang and W.-Y. Wu, BMC Complement. Altern. Med., 13, 150 (2013); doi:10.1186/1472-6882-13-150.

L.L. Lin, A.J. Liu, J.G. Liu, X.-H. Yu, L.-P. Qin and D.-F. Su, J. Cardiovasc. Pharmacol., 50, 327 (2007); doi:10.1097/FJC.0b013e3180cbd0e7.

H.F. Zhang, X.M. Hu, L.X. Wang, S.-Q. Xu and F.-D. Zeng, Planta Med., 75, 121 (2009); doi:10.1055/s-0028-1088368.

C. Gao, J. Liu, Y. Jia, L.-N. Xue, R.-L. Wang, D.-K. Li, F.-H. Jia and J.-M. Yan, China Pharmacy, 27, 2156 (2008).

R. Fang and S. Du, Chinese J. Exp. Tradit. Medical Formulae, 4, 077 (2011).

S.H. Li, X.H. Jiang, Q.J. Yang and Z.H. Jin, J. Biomed. Eng., 4, 033 (2003).

G.E.Q. Hua, Z. Zhou, X.J. Zhi, L.L. Ma and X.H. Chen, Chinese J. Pharmaceuticals, 12, (2003).

Y.H. Chao, X.M. Xu and J.N. Yu, Chinese Pharm. Affairs, 7, 032 (2010).

Y. Shao, Q.N. Ping, F. Cao and J.X. Guo, Chinese J. Natural Med., 3, (2007).

M. Gao, W. Huang, M. Roy Chowdhury and C. Liu, Anal. Chim. Acta, 591, 161 (2007); doi:10.1016/j.aca.2007.04.004.

X. Hao, G. Cheng, J. Sun, M. Zou, J. Yu, S. Zhang and F. Cui, J. Pharm. Biomed. Anal., 38, 360 (2005); doi:10.1016/j.jpba.2005.01.004.

S. Yulan and F. Fang, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 830, 1 (2006); doi:10.1016/j.jchromb.2005.10.016.

Y. Zhang, R. Gao, J.-X. Liu and S.-C. Wang, Chinese J. Clin. Pharmacol., 23, 299 (2007).

M. Gao, W. Huang and C.Z. Liu, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 858, 22 (2007); doi:10.1016/j.jchromb.2007.07.046.

Issue

Vol. 27 No. 1 (2015): Vol 27 Issue 1

Separation/Preconcentration and Determination of Scutellarin in Urine by Vortex-Assisted Surfactant-Enhanced Emulsification Microextraction and High Performance Liquid Chromatography

Corresponding Author(s) : Yaling Yang

Abstract

Keywords

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