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Vol. 28 No. 10 (2016): Vol 28 Issue 10

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in vivo Study of Central Nervous System Depressant Effects and Muscle Relaxant Activity of Methanolic Extract of Galphimia gracilis Leaf on Swiss Albino Mice

https://doi.org/10.14233/ajchem.2016.19978
Baba Shankar Rao Garige1
1Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Hyderabad-501 301, India 2University College of Pharmaceutical Sciences, Satavahana University, Karimnagar-505 002, India 3Department of Pharmacy, C.M.R. Group of Institutions, Hyderabad-501 401, India *Corresponding author: E-mail: ksrisailam@yahoo.com
Keshetti Srisailam2
1Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Hyderabad-501 301, India 2University College of Pharmaceutical Sciences, Satavahana University, Karimnagar-505 002, India 3Department of Pharmacy, C.M.R. Group of Institutions, Hyderabad-501 401, India *Corresponding author: E-mail: ksrisailam@yahoo.com
Uma Maheshwara Rao Vattikuti3
1Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Hyderabad-501 301, India 2University College of Pharmaceutical Sciences, Satavahana University, Karimnagar-505 002, India 3Department of Pharmacy, C.M.R. Group of Institutions, Hyderabad-501 401, India *Corresponding author: E-mail: ksrisailam@yahoo.com

Asian Journal of Chemistry, Vol. 28 No. 10 (2016): Vol 28 Issue 10

Abstract

This study assesses the central nervous system depressant effects and muscle relaxant activity of methanolic extract of Galphimia gracilis leaf in Swiss albino mice employing models like open field test, hole-board test, sodium pentobarbital-induced sleep test, pentylenetetrazole induced convulsions, picrotoxin-induced convulsions, rota rod test and grip strengthening test. The LD50 results of the extract was found to be > 2000 mg/kg. The extract exhibited significant (P £ 0.001) effects in both the open field test and hole-board test. The extract at 100, 200 and 400 mg/kg doses extended the duration of sleep induced by sodium pentobarbital (40 mg/kg) and also delayed the onset of seizures induced by pentylenetetrazole (90 mg/kg) and picrotoxin (10 mg/kg) respectively. The extract also exhibited significant (P £ 0.001) effects in Grip strengthening test and Rota rod test. The study concludes that the methanolic extract of Galphimia gracilis leaf has significant depressant activity and muscle relaxant effects.

Keywords

Central nervous system Convulsion Depression Galphimia gracilis Grip strength test Muscle relaxant activity.
Shankar Rao Garige1, B., Srisailam2, K., & Maheshwara Rao Vattikuti3, U. (2016). in vivo Study of Central Nervous System Depressant Effects and Muscle Relaxant Activity of Methanolic Extract of Galphimia gracilis Leaf on Swiss Albino Mice. Asian Journal of Chemistry, 28(10), 2315–2321. https://doi.org/10.14233/ajchem.2016.19978
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