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Vol. 35 No. 4 (2023): Vol 35 Issue 4, 2023

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Effect of Combination of Tween-80 with Amphiphilic Peptides on Physical Stability of Nanoemulsion Using Low-Energy Emulsification Technique

https://doi.org/10.14233/ajchem.2023.27569
Rajeev Kumar
Department of Food Technology, Uttaranchal University, Dehradun-248007, India
https://orcid.org/0000-0001-7070-1628
Shaik Abdul Hussain
Department of Dairy Technology, National Dairy Research Institute, Karnal-132001, India
https://orcid.org/0000-0003-3196-0656
Debopriya Purkayastha
Department of Food Technology, Uttaranchal University, Dehradun-248007, India
https://orcid.org/0009-0006-7536-5591

Corresponding Author(s) : Rajeev Kumar

rajeev.kumar267@gmail.com

Asian Journal of Chemistry, Vol. 35 No. 4 (2023): Vol 35 Issue 4, 2023

Abstract

The current study was investigated to replace Tween-80 with peptides in order to produce physical and microstructure stability of nanoemulsion. Oil phase (2%), Tween-80 (4%) and remaining aqueous phase were taken to form nanoemulsions. In combinations of Tween-80 and peptides three ratios (1:2, 2:1 and 1:1) were taken keeping the overall concentration 4% fixed. The particle size of nanoemulsion stabilized with Tween-80, peptides and combination of Tween-80-peptides (1:2, 1:1, 2:1) were 148.71 ± 1.48, 283.66 ± 10.17, 254.50 ± 14.77, 161.90 ± 3.67, 182.80 ± 5.37 nm, showing smallest particle size with 50% replacement of Tween-80 with peptides. The zeta potential data showed competitive adsorption between Tween-80 and peptides. The polydispersity index of Tween-80:peptides (1:1) had found to be 0.33 ± 0.01 where as Tween-80 and peptides stabilized nanoemulsion had 0.20 ± 0.01 and 0.30 ± 0.05. The changes in electrical conductivity occurred maximum in case of peptides and electrical conductivity decreased with increase in concentration of Tween-80 due to preventing mobility of ions. The storage stability of nanoemulsions were found better at 4 ºC, however Tween-80:peptides (1:1) and peptides stabilized nanoemulsion had particle size growth onwards 14 days at 37 ºC. The transmission electron microscopy (TEM) image of nanoemulsion Tween-80:peptides (1:1) showed uniform distribution of peptides in combination compared with individual peptides stabilized nanoemulsion.

Keywords

Particle size Micro structure Phase inversion Peptides Emulsifier combination
Kumar, R., Hussain, S. A., & Purkayastha, D. (2023). Effect of Combination of Tween-80 with Amphiphilic Peptides on Physical Stability of Nanoemulsion Using Low-Energy Emulsification Technique. Asian Journal of Chemistry, 35(4), 905–909. https://doi.org/10.14233/ajchem.2023.27569
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