Issue

Vol. 35 No. 8 (2023): Vol 35 Issue 8, 2023

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Green Synthesized Zinc Oxide Nanoparticles induced Apoptosis in Human Cervical (HeLa) and Breast (MCF-7) Cancer Cell Lines

https://doi.org/10.14233/ajchem.2023.27942
Falak Thakral
Department of Bio-Sciences and Technology, Maharishi Markandeshwar Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala-133207, India
https://orcid.org/0000-0003-0952-7178
Ajay Kumar
University Center for Research & Development, Biotechnology Engineering & Food Technology, Chandigarh University, Gharuan, Mohali-140413, India
https://orcid.org/0000-0002-5312-9823
Manik Sharma
University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali-140413, India
https://orcid.org/0000-0001-8204-9542
Rasdeep Kour
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar-143005, India
https://orcid.org/0009-0002-5059-7290
Satwinderjeet Kaur
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar-143005, India
https://orcid.org/0000-0003-2864-6471
Moyad Shahwan
Department of Clinical Sciences, College of Pharmacy and Health Sciences, Ajman University, Ajman 346, United Arab Emirates Centre of Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman 346, United Arab Emirates
https://orcid.org/0000-0001-8367-4841
Ritu Chauhan
Amity Institute of Environmental Sciences, Amity University, Sector-125, Noida-201303, India
https://orcid.org/0000-0002-9056-6619
Gurpreet Kaur Bhatia
Department of Physics, Maharishi Markandeshwar Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala-133207, India
https://orcid.org/0000-0002-9634-5366
Hardeep Singh Tuli
Department of Bio-Sciences and Technology, Maharishi Markandeshwar Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala-133207, India
https://orcid.org/0000-0003-1155-0094

Corresponding Author(s) : Hardeep Singh Tuli

hardeep.biotech@gmail.com

Asian Journal of Chemistry, Vol. 35 No. 8 (2023): Vol 35 Issue 8, 2023

Abstract

Chemotherapeutic resistance is currently one of the most prevalent problems due to the low absorption and undesirable toxicity of existing medications. Furthermore, non-specific targeting of existing medications is leading to the investigation of novel drug manufacturing strategies to eradicate chronic disorders. Present studies were performed to explore the anti-proliferative potential of synthesized zinc oxide nanoparticles (ZnO NPs) in human breast (MCF-7), human lung (A549) and human cervical (HeLa) cancer cell lines. The ZnO NPs were synthesized by using soybean seed extract. The ZnO NPs were characterized by ultraviolet-visible (UV-Vis) spectroscopy, field-emission scanning electron microscope (FESEM) and X-ray diffraction (XRD). The phytochemicals present in the soybean seed extract was evaluated using gas chromatography-mass spectroscopy (GC-MS) technique. Cytotoxicity effects of ZnO NPs, levofloxacin (LVX) and a combination of ZnO NPs-LVX against MCF-7, A549 and HeLa cancer cell lines was evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Annexin assays were performed to explore the apoptosis-inducing potential of ZnO NPs and its drug combination. This showed that tested compounds have potent cytotoxic effects dose-dependently against cancer cell lines. The potent anticancer potential of ZnO NPs-LVX is expressed to utilize in the near future as novel therapeutic strategies.

Keywords

Zinc oxide nanoparticles Soyabean Antiproliferative activity Cervical acitivity Apoptosis Annexin V-FITC assays
Thakral, F., Kumar, A., Sharma, M., Kour, R., Kaur, S., Shahwan, M., … Tuli, H. S. (2023). Green Synthesized Zinc Oxide Nanoparticles induced Apoptosis in Human Cervical (HeLa) and Breast (MCF-7) Cancer Cell Lines. Asian Journal of Chemistry, 35(8), 1979–1984. https://doi.org/10.14233/ajchem.2023.27942
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. U. Anand, A. Dey, A.K.S. Chandel, R. Sanyal, A. Mishra, D.K. Pandey, V. De Falco, A. Upadhyay, R. Kandimalla, A. Chaudhary, J.K. Dhanjal, S. Dewanjee, J. Vallamkondu and J.M. Pérez de la Lastra, Genes Dis., 10, 1367 (2022); https://doi.org/10.1016/j.gendis.2022.02.007
  2. W.M.C. van den Boogaard, D.S.J. Komninos and W.P. Vermeij, Cancers, 14, 627 (2022); https://doi.org/10.3390/cancers14030627
  3. E.R. Evans, P. Bugga, V. Asthana and R. Drezek, Mater. Today, 21, 673 (2018); https://doi.org/10.1016/j.mattod.2017.11.022
  4. S. Shariatzadeh, N. Moghimi, F. Khalafi, S. Shafiee, M. Mehrabi, S. Ilkhani, F. Tosan, P. Nakhaei, A. Alizadeh, R.S. Varma and M. Taheri, Front. Bioeng. Biotechnol., 10, 847433 (2022); https://doi.org/10.3389/fbioe.2022.847433
  5. J.-J. Xu, W.-C. Zhang, Y.-W. Guo, X.-Y. Chen and Y.-N. Zhang, Drug Deliv., 29, 664 (2022); https://doi.org/10.1080/10717544.2022.2039804
  6. I. Khan, K. Saeed and I. Khan, Arab. J. Chem., 12, 908 (2019); https://doi.org/10.1016/j.arabjc.2017.05.011
  7. M. Ozdal and S. Gurkok, ADMET DMPK, 10, 115 (2022); https://doi.org/10.5599/admet.1172
  8. E. Sánchez-López, D. Gomes, G. Esteruelas, L. Bonilla, A.L. Lopez-Machado, R. Galindo, A. Cano, M. Espina, M. Ettcheto, A. Camins, A.M. Silva, A. Durazzo, A. Santini, M.L. Garcia and E.B. Souto, Nanomaterials, 10, 292 (2020); https://doi.org/10.3390/nano10020292
  9. C. Pandit, A. Roy, S. Ghotekar, A. Khusro, M.N. Islam, T.B. Emran, S.E. Lam, M.U. Khandaker and D.A. Bradley, J. King Saud Univ. Sci., 34, 101869 (2022); https://doi.org/10.1016/j.jksus.2022.101869
  10. S. Sim and N.K. Wong, Biomed. Rep., 14, 42 (2021); https://doi.org/10.3892/br.2021.1418
  11. B. Bhardwaj, P. Singh, A. Kumar, S. Kumar and V. Budhwar, Adv. Pharm. Bull., 10, 566 (2020); https://doi.org/10.34172/apb.2020.067
  12. S. Ying, Z. Guan, P.C. Ofoegbu, P. Clubb, C. Rico, F. He and J. Hong, Environ. Technol. Innov., 26, 102336 (2022); https://doi.org/10.1016/j.eti.2022.102336
  13. O.V. Kharissova, B.I. Kharisov, C.M. Oliva González, Y.P. Méndez and I. López, R. Soc. Open Sci., 6, 191378 (2019); https://doi.org/10.1098/rsos.191378
  14. S.F. Ahmed, M. Mofijur, N. Rafa, A.T. Chowdhury, S. Chowdhury, M. Nahrin, A.B.M.S. Islam and H.C. Ong, Environ. Res., 204, 111967 (2022); https://doi.org/10.1016/j.envres.2021.111967
  15. M.B. Gawande, A. Goswami, F.-X. Felpin, T. Asefa, X. Huang, R. Silva, X. Zou, R. Zboril and R.S. Varma, Chem. Rev., 116, 3722 (2016); https://doi.org/10.1021/acs.chemrev.5b00482
  16. E. Tinajero-Díaz, D. Salado-Leza, C. Gonzalez, M. Martínez Velázquez, Z. López, J. Bravo-Madrigal, P. Knauth, F.Y. Flores-Hernández, S.E. Herrera-Rodríguez, R.E. Navarro, A. Cabrera-Wrooman, E. Krötzsch, Z.Y.G. Carvajal and R. Hernández-Gutiérrez, Pharmaceutics, 13, 1719 (2021); https://doi.org/10.3390/pharmaceutics13101719
  17. S. Raha and M. Ahmaruzzaman, Nanoscale Adv., 4, 1868 (2022); https://doi.org/10.1039/D1NA00880C
  18. A. Sirelkhatim, S. Mahmud, A. Seeni, N.H.M. Kaus, L.C. Ann, S.K.M. Bakhori, H. Hasan and D. Mohamad, Nano-Micro Lett., 7, 219 (2015); https://doi.org/10.1007/s40820-015-0040-x
  19. A. Kolodziejczak-Radzimska and T. Jesionowski, Materials, 7, 2833 (2014); https://doi.org/10.3390/ma7042833
  20. M.A. Subhan, N. Neogi and K.P. Choudhury, Nanomanufacturing, 2, 265 (2022); https://doi.org/10.3390/nanomanufacturing2040016
  21. S. Anjum, M. Hashim, S.A. Malik, M. Khan, J.M. Lorenzo, B.H. Abbasi and C. Hano, Cancers, 13, 4570 (2021); https://doi.org/10.3390/cancers13184570
  22. A.B. Mousa, R. Moawad, Y. Abdallah, M. Abdel-Rasheed and A.M.A. Zaher, Pharm. Res., (2023); https://doi.org/10.1007/s11095-023-03505-0
  23. S. Maher, S. Nisar, S.M. Aslam, F. Saleem, F. Behlil, M. Imran, M.A. Assiri, A. Nouroz, N. Naheed, Z.A. Khan and P. Aslam, ACS Omega, 8, 15920 (2023); https://doi.org/10.1021/acsomega.2c07621
  24. S.A. Hosseini Chaleshtori, M.A. Kachoie and S.M. Hashemi Jazi, Microbiol. Res., 8, 51 (2017); https://doi.org/10.4081/mr.2017.7319
  25. M. del C. Villalobos, M.J. Serradilla, A. Martín, E. Ordiales, S. Ruiz-Moyano and M.G. Córdoba, J. Sci. Food Agric., 96, 2116 (2016); https://doi.org/10.1002/jsfa.7327
  26. D. Malenèic, J. Cvejic and J. Miladinovic, J. Med. Food, 15, 89 (2012); https://doi.org/10.1089/jmf.2010.0329
  27. G. Mickisch, S. Fajta, G. Keilhauer, E. Schlick, R. Tschada and P. Alken, Urol. Res., 18, 131 (1990); https://doi.org/10.1007/BF00302474
  28. S. Talam, S.R. Karumuri and N. Gunnam, Int. Sch. Res. Notices, 2012, 372505 (2012); https://doi.org/10.5402/2012/372505
  29. H. Abd-Alla, H. A. A Taie and M.M. Abd-Elmotaleb, Asian J. Pharm. Clin. Res., 12, 523 (2019); https://doi.org/10.22159/ajpcr.2019.v12i2.30322
  30. R. Xu, D. Wang, J. Zhang and Y. Li, Chem. Asian J., 1, 888 (2006); https://doi.org/10.1002/asia.200600260
  31. N. Pantidos and L.E. Horsfall, J. Nanomed. Nanotechnol., 5, 233 (2014); https://doi.org/10.4172/2157-7439.1000233
  32. M. Pandurangan, G. Enkhtaivan and D.H. Kim, J. Photochem. Photobiol. B Biol., 158, 206 (2016); https://doi.org/10.1016/j.jphotobiol.2016.03.002
  33. M.J. Akhtar, M. Ahamed, S. Kumar, M.A. Majeed Khan, J. Ahmad and S.A. Alrokayan, Int. J. Nanomedicine, 2012, 845 (2012); https://doi.org/10.2147/IJN.S29129
  34. M.A. Franco-Molina, E. Mendoza-Gamboa, C.A. Sierra-Rivera, R.A. Gómez-Flores, P. Zapata-Benavides, P. Castillo-Tello, J.M. Alcocer-González, D.F. Miranda-Hernández, R.S. Tamez-Guerra and C. Rodríguez-Padilla, J. Exp. Clin. Cancer Res., 29, 148 (2010); https://doi.org/10.1186/1756-9966-29-148
Read More
Author biographies is not available.
Download
AJC-35-8-31
Statistic
Read Counter : 0 Download : 0