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Vol. 35 No. 8 (2023): Vol 35 Issue 8, 2023

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Antibacterial Activity of Ampelocissus latifolia Tuberous Root Extract against Diverse Microbes

https://doi.org/10.14233/ajchem.2023.28061
Kamal Kishor Rajak
Department of Medical Laboratory Technology, Bapubhai Desaibhai Patel Institute of Paramedical Sciences (BDIPS), Charotar University of Science and Technology, CHARUSAT Campus, Changa-388421, India
https://orcid.org/0009-0009-5541-751X
Pavan M. Pahilani
Department of Medical Laboratory Technology, Bapubhai Desaibhai Patel Institute of Paramedical Sciences (BDIPS), Charotar University of Science and Technology, CHARUSAT Campus, Changa-388421, India
https://orcid.org/0009-0001-0696-3022
Hemant Kumar
Department of Medical Laboratory Technology, Bapubhai Desaibhai Patel Institute of Paramedical Sciences (BDIPS), Charotar University of Science and Technology, CHARUSAT Campus, Changa-388421, India
https://orcid.org/0000-0001-7698-2301

Corresponding Author(s) : Hemant Kumar

hemantkumar.cips@charusat.ac.in

Asian Journal of Chemistry, Vol. 35 No. 8 (2023): Vol 35 Issue 8, 2023

Abstract

Microbial drug resistance is growing every day and novel drugs are need of the hour to counter the future threat of large pandemic. The present work for the first time explores the antibacterial activity of methanol, hydro-alcoholic and n-hexane extracts of Ampelocissus latifolia (A. latifolia) tuberous root against few laboratory and clinical isolates. A total of 10 microbes were screened; out of which 1 is a laboratory strain (acid-fast) and the rest 9 are clinical isolates (5 Gram-positive and 4 are Gram-negative), 2 clinical isolates were drug-resistant (1 Gram-positive and 1 Gram-negative). In this screening, methanol extract showed potential antibacterial activity against Gram-positive, Gram-negative and acid-fast bacteria with zones of inhibition measuring 18 mm, 10 mm and 8 mm, respectively. Hydro-alcoholic and n-hexane extract showed a smaller zone of inhibition compared to methanol extract and the positive control. The minimum inhibitory concentration (MIC) of methanol and the hydro-alcoholic extract of powdered tuber root was determined by the micro-broth dilution method. The minimum inhibitory concentration of methanol and hydro-alcoholic extract for Gram-positive and Gram-negative microbes varies from 0.18 mg/mL to 1.5 mg/mL. The methanol and hydro-alcoholic extracts were analyzed with FTIR, which revealed the presence of alcohol, alkanes, alkenes, alkyls, ketones and nitro groups in the methanol and hydro-alcoholic extracts of A. latifolia tuberous root. The high-performance thin-layer chromatography (HPTLC) analysis of the methanol extract indicates the presence of at least 5 different compounds with different Rf values.

Keywords

A. latifolia Antibacterial Micro-broth dilution HPTLC
Rajak, K. K., Pahilani, P. M., & Kumar, H. (2023). Antibacterial Activity of Ampelocissus latifolia Tuberous Root Extract against Diverse Microbes. Asian Journal of Chemistry, 35(8), 1951–1956. https://doi.org/10.14233/ajchem.2023.28061
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References
  1. S. Doron and S.L. Gorbach, in Eds.: H.K. (Kris) Heggenhougen, Inter-national Encyclopedia of Public Health, Elsevier, pp. 273-282 (2008); https://doi.org/10.1016/B978-012373960-5.00596-7
  2. A. Gray and F. Sharara, Lancet Glob. Health, 10, S2 (2022); https://doi.org/10.1016/S2214-109X(22)00131-0
  3. V.K. Viswanathan, Gut Microbes, 5, 3 (2014); https://doi.org/10.4161/gmic.28027
  4. C.L. Ventola, P&T, 40, 277 (2015).
  5. G.M. Rossolini, F. Arena, P. Pecile and S. Pollini, Curr. Opin. Pharmacol., 18, 56 (2014); https://doi.org/10.1016/j.coph.2014.09.006
  6. Z. Golkar, O. Bagasra and D.G. Pace, J. Infect. Dev. Ctries., 8, 129 (2014); https://doi.org/10.3855/jidc.3573
  7. M. Gross, Curr. Biol., 23, R1063 (2013); https://doi.org/10.1016/j.cub.2013.11.057
  8. A.F. Read and R.J. Woods, Evol. Med. Public Health, 2014, 147 (2014); https://doi.org/10.1093/emph/eou024
  9. P. Wikaningtyas and E.Y. Sukandar, Asian Pac. J. Trop. Biomed., 6, 16 (2016); https://doi.org/10.1016/j.apjtb.2015.08.003
  10. D. Savoia, Future Microbiol., 7, 979 (2012); https://doi.org/10.2217/fmb.12.68
  11. M.M. Pandey, S. Rastogi and A.K.S. Rawat, Evid. Based Complement. Alternat. Med., 2013, 376327 (2013); https://doi.org/10.1155/2013/376327
  12. V. Subhose, P. Srinivas and A. Narayana, Bull. Indian Inst. Hist. Med. Hyderabad, 35, 83 (2005).
  13. O. Habbal, S.S. Hasson, A.H. El-Hag, Z. Al-Mahrooqi, N. Al-Hashmi, Z. Al-Bimani, M.S. Al-Balushi and A.A. Al-Jabri, Asian Pac. J. Trop. Biomed., 1, 173 (2011); https://doi.org/10.1016/S2221-1691(11)60021-X
  14. F. Chassagne, T. Samarakoon, G. Porras, J.T. Lyles, M. Dettweiler, L. Marquez, A.M. Salam, S. Shabih, D.R. Farrokhi and C.L. Quave, Front. Pharmacol., 11, 586548 (2021); https://doi.org/10.3389/fphar.2020.586548
  15. R. Brun, M. Niehues, T.J. Schmidt, S.A. Khalid, A.J. Romanha, T.MA. Alves, M.W. Biavatti, F.B. Da Costa, S.L. de Castro, V.F. Ferreira, M.V.G. de Lacerda, J.H.G. Lago, L.L. Leon, N.P. Lopes, R.C. das Neves Amorim, I.V. Ogungbe, Curr. Med. Chem., 19, 2176 (2012); https://doi.org/10.2174/092986712800229087
  16. H.O. Edeoga, D.E. Okwu and B.O. Mbaebie, Afr. J. Biotechnol., 4, 685 (2005); https://doi.org/10.5897/AJB2005.000-3127
  17. S. Parvin, M.A. Kader, A.U. Chouduri, M.A.S. Rafshanjani and M.E. Haque, J. Pharmacogn. Phytochem., 2, 47 (2014).
  18. P.A. Pednekar and B. Raman, Asian J. Pharm. Clin. Res., 6, 157 (2013).
  19. P.A. Pednekar, V. Kulkarni and B. Raman, Int. J. Pharm. Pharm. Sci., 6, 602 (2014).
  20. A. Chaudhuri and S. Ray, J. King Saud Univ. Sci., 32, 732 (2020); https://doi.org/10.1016/j.jksus.2018.12.006
  21. A. Chaudhuri and S. Ray, J. King Saud Univ. Sci., 32, 1798 (2020); https://doi.org/10.1016/j.jksus.2020.01.046
  22. A. Chaudhuri, S. Chatterjee, A. Chaudhuri and S. Ray, J. Indian Chem. Soc., 97, 2874 (2020).
  23. C. Anwesa, R. Sanjib, C. Anwesa and R. Sanjib, Eur. J. Exp. Biol., 5, 1 (2015).
  24. A. Chaudhuri and S. Ray, IOSR J. Agric. Vet. Sci., 9, 90 (2016); 10.9790/2380-09070190100
  25. C.T. Tamilarasi, U. Subasin, S. Kavimani and B. Jaykar, Anc. Sci. Life, 20, 14 (2000).
  26. J.B. Patel, F.C. Tenover, J.D. Turnidge and J.H. Jorgensen, Manual of clinical microbiology., 2011, 1122 (2011); https://doi.org/10.1128/9781555816728.ch68
  27. M. Elshikh, S. Ahmed, S. Funston, P. Dunlop, M. McGaw, R. Marchant and I.M. Banat, Biotechnol. Lett., 38, 1015 (2016); https://doi.org/10.1007/s10529-016-2079-2
  28. T.A. Maryutina, E.Y. Savonina, P.S. Fedotov, R.M. Smith, H. Siren and D.B. Hibbert, Pure Appl. Chem., 90, 181 (2018); https://doi.org/10.1515/pac-2017-0111
  29. N. Tuyiringire, I. Taremwa Mugisha, D. Tusubira, J.-P. Munyampundu, C. Mambo Muvunyi and Y. Vander Heyden, J. Clin. Tuberc. Other Mycobact. Dis., 27, 100307 (2022); https://doi.org/10.1016/j.jctube.2022.100307
  30. A. Miernik and M. Rzeczycka, Pol. J. Microbiol., 56, 265 (2007).
  31. Y. Hu, L. Liu, X. Zhang, Y. Feng and Z. Zong, Front. Microbiol., 8, 2275 (2017); https://doi.org/10.3389/fmicb.2017.02275
  32. K. Ratananikom and N. Srikacha, J. Acute Dis., 9, 223 (2020); https://doi.org/10.4103/2221-6189.291288
  33. N.L. Maia, M. De Barros, L.L. De Oliveira, S.A. Cardoso, M.H. Dos Santos, F.A. Pieri, T.C. Ramalho, E.F. Da Cunha and M.A. Moreira, Front. Microbiol., 9, 1203 (2018); https://doi.org/10.3389/fmicb.2018.01203
  34. E.S. Teoh, Secondary Metabolites of Plants. In: Medicinal Orchids of Asia, Springer, Cham., p. 59 (2016); https://doi.org/10.1007/978-3-319-24274-3_5
  35. K. Snehlata, R. Sheel and B. Kumar, J. Biotechnol. Biochem., 4, 31 (2018); 10.9790/264X-0405013138
  36. B. Spangenberg, A. Seigel and R. Brämer, J. Planar Chromatogr. Mod. TLC, 35, 313 (2022); https://doi.org/10.1007/s00764-022-00176-2
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