Issue

Vol. 35 No. 10 (2023): Vol 35 Issue 10, 2023

Copyright (c) 2023 SATHISH KUMAR KONIDALA, SIVA SHANMUGAM S, S. NARAYANAN, R.K. SHANDIL, PAVAN KUMAR PURAM, RAMU SAMINENI, VEERA SHAKAR PULUSU, KAMMA HARSHA SRI

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Development and Validation of UPLC-MS/MS Method for Simultaneous Determination of Multi-Class Antibiotics, Antivirals and Antifungals in Water

UPLC-MS/MS method for determination of multi-class antimicrobials
https://doi.org/10.14233/ajchem.2023.28186
SATHISH KUMAR KONIDALA
Department of Pharmaceutical Sciences, School of Biotechnology and Pharmaceutical Sciences, Vignan’s Foundation for Science Technology and Research, Vadlamudi, Guntur-522213, Andhra Pradesh, India
https://orcid.org/0000-0002-8250-3570
SIVA SHANMUGAM S
1. DMPK and Bioanalytical Department, Foundation for Neglected Disease Research, Veerapura Industrial Village, Doddaballapur, Bengaluru- 561203, India 2. Department of Pharmaceutical Sciences, School of Biotechnology and Pharmaceutical Sciences, Vignan’s Foundation for Science Technology and Research, Vadlamudi, Guntur-522213, India
https://orcid.org/0000-0001-9188-8276
S. NARAYANAN
DMPK and Bioanalytical Department, Foundation for Neglected Disease Research, Veerapura Industrial Village, Doddaballapur, Bengaluru- 561203, India
https://orcid.org/0000-0002-7781-7399
R.K. SHANDIL
DMPK and Bioanalytical Department, Foundation for Neglected Disease Research, Veerapura Industrial Village, Doddaballapur, Bengaluru- 561203, India
https://orcid.org/0000-0002-0908-1340
PAVAN KUMAR PURAM
DMPK and Bioanalytical Department, Foundation for Neglected Disease Research, Veerapura Industrial Village, Doddaballapur, Bengaluru- 561203, India
https://orcid.org/0009-0006-5470-5139
RAMU SAMINENI
2. Department of Pharmaceutical Sciences, School of Biotechnology and Pharmaceutical Sciences, Vignan’s Foundation for Science Technology and Research, Vadlamudi, Guntur-522213, India 3. Department of Pharmaceutics, School of Pharmacy, Sandip University, Nashik-422213, India
https://orcid.org/0000-0002-8652-2883
VEERA SHAKAR PULUSU
Department of Chemistry & Biochemistry, Ohio University, Athens, OH 45701, U.S.A.
https://orcid.org/0000-0003-1801-6388
KAMMA HARSHA SRI
Department of Pharmaceutical Analysis, Vignan Pharmacy College, Vadlamudi, Guntur-522213, India
https://orcid.org/0009-0009-5329-3162

Corresponding Author(s) : SATHISH KUMAR KONIDALA

sathishkonidala@gmail.com

Asian Journal of Chemistry, Vol. 35 No. 10 (2023): Vol 35 Issue 10, 2023

Abstract

According to a WHO report, antimicrobial resistance is one of the top 10 health threats. Environmental sources are extremely contaminated with high levels of antibiotics, antivirals and antifungals due to their excessive and unsafe usage. Hence, there is a need to detect and quantitate the antimicrobial agents in water. Consequently, a simultaneous analytical method is developed and validated, employing a solid-phase extraction methodology with an HLB cartridge. This method was utilized to quantify a minimum of twenty different chemicals derived from antibiotics, antivirals and antifungal drugs. Antibiotics include quinolones, isonicotinic acid, macrolides, oxazolidinones, penicillin, sulphonamides, antivirals that contain nucleotide class compounds and antifungals that comprising of triazoles and imidazoles. Ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC- S/MS) was used to quantify the analytes. Detection was achieved using multiple reaction monitoring with positive electron spray  ionization. All the compounds were well separated with the column, Xterra MS C18 4.6 × 50 mm 2.5 μm, using water and acetonitrile with 0.1% formic acid as a mobile phase. The calibration curve range was set at 50 to 2000 ng mL-1. Validation parameters like precision and accuracy, matrix effect, sensitivity, autosampler stability, the limit of detection and recovery were performed.

Keywords

Antimicrobials Antimicrobials UPLC-MS/MS UPLC-MS/MS Solid-phase extraction Solid-phase extraction Antimicrobial resistance Antimicrobial resistance Validation Validation
KONIDALA, S. K., SHANMUGAM S, S., NARAYANAN, S., SHANDIL, R., PURAM, P. K., SAMINENI, R., … SRI, K. H. (2023). Development and Validation of UPLC-MS/MS Method for Simultaneous Determination of Multi-Class Antibiotics, Antivirals and Antifungals in Water: UPLC-MS/MS method for determination of multi-class antimicrobials. Asian Journal of Chemistry, 35(10), 2509–2516. https://doi.org/10.14233/ajchem.2023.28186
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. B. Tornimbene, S. Eremin, R. Abednego, E.O. Abualas, I. Boutiba, A. Egwuenu, L. Gahimbare, W. Kasambara, C. Lukwesa-Musyani, W. Fuller, S. Githii, F.A. Miamina, S. Mtapuri-Zinyowera, G. Najjuka, O. Perovic, B. Zayed, Y.A. Ahmed, M.T. Ismail and C.L. Pessoa da Silva, Afr. J. Lab. Med., 11, 1 (2022); https://doi.org/10.4102/ajlm.v11i1.1594
  2. W. Calero-Cáceres, E. Marti, J. Olivares-Pacheco and L. Rodriguez-Rubio, Front. Microbiol., 13, 866268 (2022); https://doi.org/10.3389/fmicb.2022.866268
  3. S. Suzuki, A. Pruden, M. Virta and T. Zhang, Front. Microbiol., 8, 14 (2017); https://doi.org/10.3389/fmicb.2017.00014
  4. T.C.M.V. Do, D.Q. Nguyen, T.D. Nguyen and P.H. Le, Catalysts, 10, 356 (2020); https://doi.org/10.3390/catal10030356
  5. K. El Hawari, S. Mokh, S. Doumyati, M. Al Iskandarani and E. Verdon, Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess., 34, 582 (2017); https://doi.org/10.1080/19440049.2016.1232491
  6. B. Schwaiger, J. König and C. Lesueur, Food Anal. Methods, 11, 1417 (2018); https://doi.org/10.1007/s12161-017-1101-1
  7. G. Alija, Z. Hajrulai-Musliu and R. Uzunov, SN Appl. Sci., 2, 1563 (2020); https://doi.org/10.1007/s42452-020-03361-2
  8. A. Lakew, T. Assefa, M. Woldeyohannes, B.S. Chandravanshi and N. Megersa, BMC Chem., 16, 5 (2022); https://doi.org/10.1186/s13065-022-00797-y
  9. D. Chan, J. Tarbin, M. Sharman, M. Carson, M. Smith and S. Smith, Anal. Chim. Acta, 700, 194 (2011); https://doi.org/10.1016/j.aca.2010.11.015
  10. G. Míguez-Suárez, A. Cardelle-Cobas, L. Sinisterra-Loaiza, B. Vázquez, A. Cepeda and C. Nebot, Molecules, 27, 1474 (2022); https://doi.org/10.3390/molecules27051474
  11. E. Patyra and K. Kwiatek, Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess., 34, 1553 (2017); https://doi.org/10.1080/19440049.2017.1364430
  12. J. Kazakova, R. Fernández-Torres, M. Ramos-Payán and M.Á. Bello-López, J. Pharm. Biomed. Anal., 160, 144 (2018); https://doi.org/10.1016/j.jpba.2018.07.057
  13. C.-L. Chan, H.K.-F. Wai, P. Wu, S.-W. Lai, O.S.-K. Chan and H.M. Tun, Antibiotics, 11, 845 (2022); https://doi.org/10.3390/antibiotics11070845
  14. H.A. Assress, H. Nyoni, B.B. Mamba and T.A.M. Msagati, Ecotoxicol. Environ. Saf., 187, 109868 (2020); https://doi.org/10.1016/j.ecoenv.2019.109868
  15. E.M. Stevenson, W.H. Gaze, N.A. Gow, A. Hart, W. Schmidt, J. Usher, A. Warris, H. Wilkinson and A.K. Murray, Front. Fungal Biol., 3, 918717 (2022); https://doi.org/10.3389/ffunb.2022.918717
  16. H.A. Assress, R. Selvarajan, H. Nyoni, H.J. Ogola, B.B. Mamba and T.A. Msagati, Environ. Sci. Pollut. Res. Int., 28, 3217 (2021); https://doi.org/10.1007/s11356-020-10688-1
  17. Environment Agency, Scoping Review into Environmental Selection for Antifungal Resistance and Testing Methodology, Environment Agency, Bristol (2022).
  18. T.K. Burki, Lancet Respir. Med., 10, e18 (2022); https://doi.org/10.1016/S2213-2600(22)00011-X
  19. C. Nannou, A. Ofrydopoulou, E. Evgenidou, D. Heath, E. Heath and D. Lambropoulou, Sci. Total Environ., 699, 134322 (2020); https://doi.org/10.1016/j.scitotenv.2019.134322
  20. K. Kuroda, C. Li, K. Dhangar and M. Kumar, Sci. Total Environ., 776, 145740 (2021); https://doi.org/10.1016/j.scitotenv.2021.145740
  21. O.A. Abafe, J. Späth, J. Fick, S. Jansson, C. Buckley, A. Stark, B. Pietruschka and B.S. Martincigh, Chemosphere, 200, 660 (2018); https://doi.org/10.1016/j.chemosphere.2018.02.105
  22. M. Gros, S. Rodríguez-Mozaz and D. Barceló, J. Chromatogr. A, 1292, 173 (2013); https://doi.org/10.1016/j.chroma.2012.12.072
  23. E. De, B.S. Abdallah and M. Hilali, Pharm. Anal. Acta, 9, 1 (2018); https://doi.org/10.4172/2153-2435.1000578
  24. E. Ngumba, P. Kosunen, A. Gachanja and T. Tuhkanen, Anal. Methods, 8, 6720 (2016); https://doi.org/10.1039/C6AY01695B
  25. A. Mostafa, H. Shaaban, K. Alasmry, A. Alshammari, R. Alsuwaigh and O. Aga, J. Anal. Pharm. Res., 7, 611 (2018); https://doi.org/10.15406/japlr.2018.07.00291
  26. R. Mirzaei, M. Yunesian, S. Nasseri, M. Gholami, E. Jalilzadeh, S. Shoeibi, H.S. Bidshahi and A. Mesdaghinia, J. Environ. Health Sci. Eng., 15, 21 (2017); https://doi.org/10.1186/s40201-017-0282-2
  27. A. Mishra, Y.S. Chhonker, A.C. Bisen, Y.D. Prasad, S.L. Tulsankar, H. Chandasana, T. Dey, S.K. Verma, V. Bala, S. Kanojiya, S. Ghatak and R.S. Bhatta, ACS Omega, 5, 31584 (2020); https://doi.org/10.1021/acsomega.0c03863
  28. S. Han, X. Li, H. Huang, T. Wang, Z. Wang, X. Fu, Z. Zhou, P. Du and X. Li, Int. J. Environ. Res. Public Health, 18, 10640 (2021); https://doi.org/10.3390/ijerph182010640
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 0 Download : 0