Fast Separation and Determination of Four Tobacco-Specific Nitrosamines in Cigarette Smoke Using UPLC-MS/MS

Xiao-Lan Li; Yun Zhou; Zhi-Yan Chen; Qi-Bin Liu; Wen-Jing Zhu; Gui-Feng Huang; Jun Yang

doi:10.14233/ajchem.2015.18578

Issue

Vol. 27 No. 9 (2015): Vol 27 Issue 9

Issue Published : May 26, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Fast Separation and Determination of Four Tobacco-Specific Nitrosamines in Cigarette Smoke Using UPLC-MS/MS

https://doi.org/10.14233/ajchem.2015.18578

Xiao-Lan Li

Technology Center, China Tobacco Guangxi Industrial Co. LTD., Nanning 530001, P.R. China

Yun Zhou

Technology Center, China Tobacco Guangxi Industrial Co. LTD., Nanning 530001, P.R. China

Zhi-Yan Chen

Technology Center, China Tobacco Guangxi Industrial Co. LTD., Nanning 530001, P.R. China

Qi-Bin Liu

Technology Center, China Tobacco Guangxi Industrial Co. LTD., Nanning 530001, P.R. China

Wen-Jing Zhu

Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230052, P.R. China

Gui-Feng Huang

Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230052, P.R. China

Jun Yang

Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230052, P.R. China

Corresponding Author(s) : Jun Yang

yjun8202@ustc.edu.cn

Asian Journal of Chemistry, Vol. 27 No. 9 (2015): Vol 27 Issue 9
Article Published : May 26, 2015

Abstract

An ultra high pressure liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for fast separation and determination of the four major tobacco-specific nitrosamines in mainstream cigarette smoke has been developed. Cigarette smoke particulate matter was extracted with 100 mM ammonium acetate after addition of isotopically labeled internal standards, further purified by solid-phase extraction using PCX column. Subsequently, the target analysis was performed by UPLC-MS/MS. The limit of quantification (S/N > 10) for the four tobacco-specific nitrosamines were 0.23, 0.29, 0.25 and 0.35 ng/mL respectively, with a linear calibration range spanning 0.8-100 ng/mL. This method was validated by comparing the quantitative results for tobacco-specific nitrosamines with the conventional standard method.

Keywords

Tobacco-specific N-nitrosamines UPLC-MS/MS Cigarette smoke Solid-phase extraction

Li, X.-L., Zhou, Y., Chen, Z.-Y., Liu, Q.-B., Zhu, W.-J., Huang, G.-F., & Yang, J. (2015). Fast Separation and Determination of Four Tobacco-Specific Nitrosamines in Cigarette Smoke Using UPLC-MS/MS. Asian Journal of Chemistry, 27(9), 3293–3296. https://doi.org/10.14233/ajchem.2015.18578

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Issue

Vol. 27 No. 9 (2015): Vol 27 Issue 9

Fast Separation and Determination of Four Tobacco-Specific Nitrosamines in Cigarette Smoke Using UPLC-MS/MS

Corresponding Author(s) : Jun Yang

Abstract

Keywords

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