Field-Amplified on-line Sample Stacking for Separation and Determination of Adefovir and Tenofovir Using Capillary Electrophoresis

Jingyi Liu; Yixiang Duan

doi:10.14233/ajchem.2015.15412

Issue

Vol. 27 No. 9 (2015): Vol 27 Issue 9

Issue Published : May 26, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Field-Amplified on-line Sample Stacking for Separation and Determination of Adefovir and Tenofovir Using Capillary Electrophoresis

https://doi.org/10.14233/ajchem.2015.15412

Jingyi Liu

Research Center of Analytical Instrumentation, Analytical and Testing Centre, College of Chemistry, Sichuan University, No. 29 Wangjiang Road, Chengdu 610064, P.R. China

Yixiang Duan

Research Center of Analytical Instrumentation, Analytical and Testing Centre, College of Chemistry, Sichuan University, No. 29 Wangjiang Road, Chengdu 610064, P.R. China

Corresponding Author(s) : Yixiang Duan

yduan@scu.edu.cn; analcenter@163.com

Asian Journal of Chemistry, Vol. 27 No. 9 (2015): Vol 27 Issue 9
Article Published : May 26, 2015

Abstract

An on-line sample stacking method known as field amplified sample stacking, using hydroxypropylmethyl cellulose as electro-osmotic flow suppressant, was developed for separation and identification of adefovir and tenofovir for the first time. A water plug (hydrodynamic injection for 15 s, 2.0 psi) was added to the system prior to the loading of sample (electrokinetic injection at cathode end (14.6 kV, 20 s)) in the capillary (80 cm × 75 μm). The separation was performed using phosphate solution containing 0.3 % hydroxypropylmethyl cellulose and measured at 18 kV and 214 nm. Comparing with the conventional capillary electrophoresis, the signal enhancement factor was found to be 146 for adefovir (LOD = 2.67 ng mL^-1) and 137 for tenofovir (LOD = 3.22 ng mL^-1) (S/N = 3). The proposed method has potential application in pharmacokinetics and can reach detection limits comparable to mass spectrometry, a more complicated and costly procedures.

Keywords

Adefovir Tenofovir Capillary electrophoresis Field-amplified sample stacking On-line sample enrichment

Liu, J., & Duan, Y. (2015). Field-Amplified on-line Sample Stacking for Separation and Determination of Adefovir and Tenofovir Using Capillary Electrophoresis. Asian Journal of Chemistry, 27(9), 3143–3148. https://doi.org/10.14233/ajchem.2015.15412

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References

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References

R.E. Vryheid, M.A. Kane, N. Muller, G.C. Schatz and S. Bezabeh, Vaccine, 19, 1026 (2000); doi:10.1016/S0264-410X(00)00239-5.

P. Barditch-Crovo, S.G. Deeks, A. Collier, S. Safrin, D.F. Coakley, M. Miller, B.P. Kearney, R.L. Coleman, P.D. Lamy, J.O. Kahn, I. McGowan and P.S. Lietman, Antimicrob. Agents Chemother., 45, 2733 (2001); doi:10.1128/AAC.45.10.2733-2739.2001.

S.G. Deeks, P. Barditch-Crovo, P.S. Lietman, F. Hwang, K.C. Cundy, J.F. Rooney, N.S. Hellmann, S. Safrin, and J.O. Kahn, Antimicrob. Agents Chemother., 42, 2380 (1998).

M. Louie, C. Hogan, A. Hurley, V. Simon, C. Chung, N. Padte, P. Lamy, J. Flaherty, D. Coakley, M. Di Mascio, A.S. Perelson and M. Markowitz, AIDS, 17, 1151 (2003); doi:10.1097/00002030-200305230-00006.

Y. Benhamou, R. Tubiana and V. Thibault, N. Engl. J. Med., 348, 177 (2003); doi:10.1056/NEJM200301093480218.

R. Bruno, P. Sacchi, C. Zocchetti, V. Ciappina, M. Puoti and G. Filice, AIDS, 17, 783 (2003); doi:10.1097/00002030-200303280-00028.

A. Kuo, J.L. Dienstag and R.T. Chung, Clin. Gastroenterol. Hepatol., 2, 266 (2004); doi:10.1016/S1542-3565(04)00017-5.

M. Nelson, S. Portsmouth, J. Stebbing, M. Atkins, A. Barr, G. Matthews, D. Pillay, M. Fisher, M. Bower and B. Gazzard, AIDS, 17, F7 (2003); doi:10.1097/00002030-200301030-00002.

M. Núñez, M. Pérez-Olmeda, B. Díaz, P. Ríos, J. González-Lahoz and V. Soriano, AIDS, 16, 2352 (2002); doi:10.1097/00002030-200211220-00023.

S. Menne, P.J. Cote, B.E. Korba, S.D. Butler, A.L. George, I.A. Tochkov, W.E. Delaney, S. Xiong, J.L. Gerin and B.C. Tennant, Antimicrob. Agents Chemother., 49, 2720 (2005); doi:10.1128/AAC.49.7.2720-2728.2005.

H.T. Xie, G.J. Wang, M.J. Xu, Y.-W. Jia, H. Li, J.-G. Sun and P. Li, Chromatographia, 71, 587 (2010); doi:10.1365/s10337-010-1474-4.

J.F. Guo, F.H. Meng, L. Li, B. Zhong and Y. Zhao, Biol. Pharm. Bull., 34, 877 (2011); doi:10.1248/bpb.34.877.

T. Delahunty, L. Bushman and C.V. Fletcher, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 830, 6 (2006); doi:10.1016/j.jchromb.2005.10.015.

K. Vavrova, K. Lorencova, J. Klimentova, J. Novotný and A. Hrabálek, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 853, 198 (2007); doi:10.1016/j.jchromb.2007.03.012.

V. Jullien, J.M. Treluyer, G. Pons and E. Rey, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 785, 377 (2003); doi:10.1016/S1570-0232(02)00933-9.

N.L. Rezk, R.D. Crutchley and A.D.M. Kashuba, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 822, 201 (2005); doi:10.1016/j.jchromb.2005.06.019.

V. Kostal, J. Katzenmeyer and E.A. Arriaga, Anal. Chem., 80, 4533 (2008); doi:10.1021/ac8007384.

C. Johns, M.C. Breadmore, M. Macka, M. Ryvolová and P.R. Haddad, Electrophoresis, 30(S1), S53 (2009); doi:10.1002/elps.200900103.

R.T. Kennedy, I. German, J.E. Thompson and S.R. Witowski, Chem. Rev., 99, 3081 (1999); doi:10.1021/cr980134j.

J.P. Quirino, J.-B. Kim and S. Terabe, J. Chromatogr. A, 965, 357 (2002); doi:10.1016/S0021-9673(01)01499-6.

Y. Zhao, K. McLaughlin and C.E. Lunte, Anal. Chem., 70, 4578 (1998); doi:10.1021/ac980427c.

E. van der Vlis, M. Mazereeuw, U.R. Tjaden, H. Irth and J. van der Greef, J. Chromatogr. A, 687, 333 (1994); doi:10.1016/0021-9673(94)00776-4.

A.K. Korir, V.K. Almeida, D.S. Malkin and C.K. Larive, Anal. Chem., 77, 5998 (2005); doi:10.1021/ac050669u.

A. Riaz, B. Kim and D.S. Chung, Electrophoresis, 24, 2788 (2003); doi:10.1002/elps.200305537.

W.F. Smyth, G.B. Harland, S. McClean, G. McGrath and D. Oxspring, J. Chromatogr. A, 772, 161 (1997); doi:10.1016/S0021-9673(97)00182-9.

J.P. Quirino and S. Terabe, Electrophoresis, 21, 355 (2000); doi:10.1002/(SICI)1522-2683(20000101)21:2<355::AID-ELPS355>3.0.CO;2-D.

C.-X. Zhang and W. Thormann, Anal. Chem., 68, 2523 (1996); doi:10.1021/ac951250e.

J.C. Sanders, M.C. Breadmore, Y.C. Kwok, K.M. Horsman and J.P. Landers, Anal. Chem., 75, 986 (2003); doi:10.1021/ac020425z.

R.-L. Chien and D.S. Burgi, J. Chromatogr. A, 559, 141 (1991); doi:10.1016/0021-9673(91)80066-P.

J.Z. Song, J. Chen, S.J. Tian and Z.-P. Sun, J. Pharm. Biomed. Anal., 21, 569 (1999); doi:10.1016/S0731-7085(99)00158-2.

A. Zinellu, S. Sotgia, L. Deiana and C. Carru, Electrophoresis, 32, 1893 (2011); doi:10.1002/elps.201100056.

X.L. Hou, D.L. Deng, X. Wu, Y. Lv and J. Zhang, J. Chromatogr. A, 1217, 5622 (2010); doi:10.1016/j.chroma.2010.06.070.

Y.F. Shi, Y. Huang, J.P. Duan, H. Chen and G. Chen, J. Chromatogr. A, 1125, 124 (2006); doi:10.1016/j.chroma.2006.04.061.

Issue

Vol. 27 No. 9 (2015): Vol 27 Issue 9

Field-Amplified on-line Sample Stacking for Separation and Determination of Adefovir and Tenofovir Using Capillary Electrophoresis

Corresponding Author(s) : Yixiang Duan

Abstract

Keywords

Full Article

Download Citation

References

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