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Spectrofluorimetric and Spectrophotometric Determination of Troxerutin in Pharmaceutical Preparations
Corresponding Author(s) : Ya Hong Chen
Asian Journal of Chemistry,
Vol. 27 No. 5 (2015): Vol 27 Issue 5
Abstract
Two new, sensitive and simple spectrofluorimetric and spectrophotometric methods have been developed for the determination of troxerutin in pharmaceutical preparations. Troxerutin inhibits the activity of hemoglobin on the catalyzed reaction of H2O2 with rhodamine B, which is a highly sensitive fluorogenic and chromogenic reagent used in many investigations. The reaction product was measured by spectrofluorimetry at 575 nm after excitation at 550 nm. The percentage inhibition was directly proportional to the concentration over the range 0.04-1.5 μg/mL for the spectrofluorimetric method. The relation between the percentage inhibition of the absorbance at 550 nm and the concentration is rectilinear over the range 0.2-30 μg/mL. This method was applied successfully to the determination of troxerutin in pharmaceutical dosage form.
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- R. Bruppacher, B. Rieckemann, B. Naser-Hijazi and P. Wustenberg, Pharmacoepidemiol. Drug Saf., 7(S1), 37 (1998); doi:10.1002/(SICI)1099-1557(199808)7:1+3.3.CO;2-A.
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- D.K. Maurya, S. Balakrishnan, V.P. Salvi and C.K. Nair, Mol. Cell. Biochem., 280, 57 (2005); doi:10.1007/s11010-005-8052-3.
- M.R. Boisseau, A. Taccoen, C. Garreau and C. Vergnes, J. Cardiovasc. Surg., 36, 369 (1995).
- S. Schuller-Petrovic, M. Wolzt, K. Bo¨hler, B. Jilma and H.-G. Eichler, Clin. Pharmacol. Ther., 56, 452 (1994); doi:10.1038/clpt.1994.160.
- F. Squadrito, D. Altavilla and S.O. Bosso, Eur. Rev. Med. Pharmacol. Sci., 4, 21 (2000).
- China Pharmacopoeia Committee, Pharmacopoeia of People's Republic of China, Chemical Industry Press, Beijing, Vol. 2, p. 92 (2005).
- Y.H. Chen and F.S. Tian, Luminescence, 27, 59 (2012); doi:10.1002/bio.1325.
- Z. Chen, J. Liu, Y. Han and L. Zhu, Chin. J. Anal. Lab., 570, 109 (2006); doi:10.1016/j.aca.2006.04.003.
- X.Y. Cui, M.L. Zhang, X. Guan, L. Yin, Y. Sun, J.P. Fawcett and J. Gu, Chromatographia, 73, 165 (2011); doi:10.1007/s10337-010-1827-z.
- G.B. Jameson, F.S. Molinaro, J.A. Ibers, J.P. Collman, J.I. Brauman, E. Rose and K.S. Suslick, J. Am. Chem. Soc., 102, 3224 (1980); doi:10.1021/ja00529a055.
- K. Zhang, R. Cai, D. Chen and L. Mao, Anal. Chim. Acta, 413, 109 (2000); doi:10.1016/S0003-2670(00)00752-2.
References
R. Bruppacher, B. Rieckemann, B. Naser-Hijazi and P. Wustenberg, Pharmacoepidemiol. Drug Saf., 7(S1), 37 (1998); doi:10.1002/(SICI)1099-1557(199808)7:1+3.3.CO;2-A.
A. Russo, R. Acquaviva, A. Campisi, V. Sorrenti, C. Di Giacomo, G. Virgata, M.L. Barcellona and A. Vanella, Cell Biol. Toxicol., 16, 91 (2000); doi:10.1023/A:1007685909018.
D.K. Maurya, S. Balakrishnan, V.P. Salvi and C.K. Nair, Mol. Cell. Biochem., 280, 57 (2005); doi:10.1007/s11010-005-8052-3.
M.R. Boisseau, A. Taccoen, C. Garreau and C. Vergnes, J. Cardiovasc. Surg., 36, 369 (1995).
S. Schuller-Petrovic, M. Wolzt, K. Bo¨hler, B. Jilma and H.-G. Eichler, Clin. Pharmacol. Ther., 56, 452 (1994); doi:10.1038/clpt.1994.160.
F. Squadrito, D. Altavilla and S.O. Bosso, Eur. Rev. Med. Pharmacol. Sci., 4, 21 (2000).
China Pharmacopoeia Committee, Pharmacopoeia of People's Republic of China, Chemical Industry Press, Beijing, Vol. 2, p. 92 (2005).
Y.H. Chen and F.S. Tian, Luminescence, 27, 59 (2012); doi:10.1002/bio.1325.
Z. Chen, J. Liu, Y. Han and L. Zhu, Chin. J. Anal. Lab., 570, 109 (2006); doi:10.1016/j.aca.2006.04.003.
X.Y. Cui, M.L. Zhang, X. Guan, L. Yin, Y. Sun, J.P. Fawcett and J. Gu, Chromatographia, 73, 165 (2011); doi:10.1007/s10337-010-1827-z.
G.B. Jameson, F.S. Molinaro, J.A. Ibers, J.P. Collman, J.I. Brauman, E. Rose and K.S. Suslick, J. Am. Chem. Soc., 102, 3224 (1980); doi:10.1021/ja00529a055.
K. Zhang, R. Cai, D. Chen and L. Mao, Anal. Chim. Acta, 413, 109 (2000); doi:10.1016/S0003-2670(00)00752-2.