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Investigation of Solvent-Dependent Catalytic Behaviour of Hydrophobic Guest Artificial Glutathione Peroxidase Using Cumene Hydroperoxide and 4-Nitrobenzenethiol as Substrates
Corresponding Author(s) : Y.Z. Yin
Asian Journal of Chemistry,
Vol. 27 No. 5 (2015): Vol 27 Issue 5
Abstract
The catalytic behaviour of a hydrophobic guest glutathione peroxidase (ADA-Te-ADA) was detailed investigated using cumene hydroperoxide and 4-nitrobenzenethiol as substrates. The relation between the catalytic rate of artificial glutathione peroxidase and the property of solvent used in the determination of catalytic rate was revealed. Herein, C2H5OH, DMSO, DMF and CH3CN were selected as the co-solvents in the determination of catalytic rates. The typical solvent-dependent catalytic behaviour of ADA-Te-ADA was exhibited. Especially, the higher catalytic rate was observed when polar protic solvent (ethanol) was used compared with other co-solvents. It suggested that polar protic solvent was the appropriate co-solvent for the assay of catalytic activity of hydrophobic artificial glutathione peroxidase. Additionally, the strong polarity of polar aprotic solvent plays an important role in the enhancement of glutathione peroxidase catalytic activity. This study suited well for the understanding of the catalytic behaviour of hydrophobic guest artificial glutathione peroxidase
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References
L. Flohé, G. Loschen, W.A. Günzler and E. Eichele, Hoppe Seylers Z. Physiol. Chem., 353, 987 (1972); doi:10.1515/bchm2.1972.353.1.987.
N. Ezirmik, S. Taysi, R. Celik, G. Celik, H.A. Alici, H. Turhan, M. Cesur and D. Keskin, Asian. J. Chem., 20, 1950 (2008).
A. Cebi, E. Diraman and Z. Eren, Asian. J. Chem., 21, 1359 (2009).
Y. Yin, L. Wang, H. Jin, C. Lv, S. Yu, X. Huang, Q. Luo, J. Xu and J. Liu, Soft Matter, 7, 2521 (2011); doi:10.1039/c0sm01081b.
Y. Yin, Z. Dong, Q. Luo and J. Liu, Prog. Polym. Sci., 37, 1476 (2012); doi:10.1016/j.progpolymsci.2012.04.001.
H. Sies and H. Masumoto, Adv. Pharmacol., 38, 229 (1996); doi:10.1016/S1054-3589(08)60986-2.
B.K. Sarma and G. Mugesh, J. Am. Chem. Soc., 127, 11477 (2005); doi:10.1021/ja052794t.
X. Zhang, H. Xu, Z. Dong, Y. Wang, J. Liu and J. Shen, J. Am. Chem. Soc., 126, 10556 (2004); doi:10.1021/ja048890w.
S. Yu, X. Huang, L. Miao, J. Zhu, Y. Yin, Q. Luo, J. Xu, J. Shen and J. Liu, Bioorg. Chem., 38, 159 (2010); doi:10.1016/j.bioorg.2010.03.001.
Y.Z. Yin, C. Lang, X.X. Hu, Z.F. Shi, Y. Wang, S.F. Jiao, C.X. Cai and J.Q. Liu, Russ. J. Bioorgan. Chem., 40, 162 (2014); doi:10.1134/S1068162014010142.
Z.P. Wu and D. Hilvert, J. Am. Chem. Soc., 112, 5647 (1990); doi:10.1021/ja00170a043.