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Vol. 27 No. 5 (2015): Vol 27 Issue 5

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Extraction and Separation of Polysaccharides from Radix isatidis and their Effects Towards Hemagglutinin Protein of Influenza Virus

https://doi.org/10.14233/ajchem.2015.17394
Zi-Feng Yang
State Key Laboratory of Respiratory Disease (Guangzhou Medical University, China), National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Road, Guangzhou, P.R. China; Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau SAR, P.R. China
Ping Hu
East China University of Science and Technology, 130th Meilong Road, Xuhui District, Shanghai, P.R. China
Shan Zhong
State Key Laboratory of Respiratory Disease (Guangzhou Medical University, China), National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Road, Guangzhou, P.R. China
Ye Chen
East China University of Science and Technology, 130th Meilong Road, Xuhui District, Shanghai, P.R. China
Li Li
State Key Laboratory of Respiratory Disease (Guangzhou Medical University, China), National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Road, Guangzhou, P.R. China
Xu-Zhao Li
State Key Laboratory of Respiratory Disease (Guangzhou Medical University, China), National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Road, Guangzhou, P.R. China
Chuyan Li
Guangzhou Institute of Pharmaceutical Industry, Guangzhou 510240, P.R. China
Jing Li
State Key Laboratory of Respiratory Disease (Guangzhou Medical University, China), National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Road, Guangzhou, P.R. China
Yu-Tao Wang
State Key Laboratory of Respiratory Disease (Guangzhou Medical University, China), National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang Road, Guangzhou, P.R. China

Corresponding Author(s) : Zi-Feng Yang

jeffyah@163.com

Asian Journal of Chemistry, Vol. 27 No. 5 (2015): Vol 27 Issue 5

Abstract

In this study, the anti-influenza virus effects of the polysaccharides of Radix isatidis and its possible target were explored. The hot water reflux extraction and stepwise ethanol precipitation were performed to extract and separate the polysaccharides from R. isatidis, the isolated constituents' activities against the influenza virus were subsequently evaluated through in vitro experiments. The ELISA assay was performed to verify the binding ability of 80 % alcohol-precipitated crude polysaccharides against the hemagglutinin (HA) of influenza A virus (A/PR/8/34, H1N1). The 70, 80 and 90 % alcohol-precipitated crude polysaccharides extracted from R. isatidis exhibited the anti-influenza virus activities,with the selection indexes (SI) as 2.69,7.52 and 5.75, respectively. Furthermore, the ELISA results showed that this constituent existed the specific binding capacity with the influenza virus HA with a dose-dependent manner. The 80 % alcohol-precipitated crude polysaccharides was one of the active components of this medicinal plant and the influenza virus HA protein might be its active target.

Keywords

Radix isatidis Polysaccharide Isolation Influenza virus hemagglutinin protein Biological activity
Yang, Z.-F., Hu, P., Zhong, S., Chen, Y., Li, L., Li, X.-Z., … Wang, Y.-T. (2015). Extraction and Separation of Polysaccharides from Radix isatidis and their Effects Towards Hemagglutinin Protein of Influenza Virus. Asian Journal of Chemistry, 27(5), 1615–1620. https://doi.org/10.14233/ajchem.2015.17394
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