Issue

Vol. 27 No. 2 (2015): Vol 27 Issue 2

Copyright (c) 2015 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Serum Metabolomic Analysis of Cervical Cancer Patients by Gas Chromatography-Mass Spectrometry

https://doi.org/10.14233/ajchem.2015.17027
Pengzhi Shi
Department of Chemistry, Capital Normal University, Beijing 100048, P.R. China
Liping Zhang
Department of Chemistry, Capital Normal University, Beijing 100048, P.R. China
Nengsheng Ye
Department of Chemistry, Capital Normal University, Beijing 100048, P.R. China

Corresponding Author(s) : Nengsheng Ye

yensh@cnu.edu.cn

Asian Journal of Chemistry, Vol. 27 No. 2 (2015): Vol 27 Issue 2

Abstract

In this paper, gas chromatography-mass spectrometry (GC-MS) in combination with pattern recognition techniques were used to analyze serum metabolites in cervical cancer patients. The experimental parameters, including the extraction solvent, temperature and time of derivatization and chromatographic conditions, were investigated for serum metabolomic profiling. Forty-five endogenous metabolites included amino acids, fatty acids, carbohydrates and other intermediate metabolites were identified. Partial least squares discriminant analysis (PLS-DA), clustering analysis and principal component analysis based on these metabolites were applied to the discrimination of health group from cervical cancer group. The results showed that 18 metabolites were detected differently between the health group and cervical cancer group, but also demonstrated that the GC-MS technique was a valuable tool for the characterization of the serum metabolic profiling and the metabolomic study will be certainly benefit for monitoring the state of cervical patients, the prognosis and therapy evaluation of cervical cancer patients.

Keywords

Cervical cancer Metabolomics GC-MS Statistical analysis
Shi, P., Zhang, L., & Ye, N. (2015). Serum Metabolomic Analysis of Cervical Cancer Patients by Gas Chromatography-Mass Spectrometry. Asian Journal of Chemistry, 27(2), 547–551. https://doi.org/10.14233/ajchem.2015.17027
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. X.D. Ma, G.Q. Cai, W. Zou, Y.H. Huang, J.R. Zhang, D.T. Wang and B.L. Chen, Gene, 524, 208 (2013); doi:10.1016/j.gene.2013.04.025.
  2. U. Siebert, G. Sroczynski, P. Hillemanns, J. Engel, R. Stabenow, C. Stegmaier, K. Voigt, B. Gibis, D. Hölzel and S.J. Goldie, Eur. J. Public Health, 16, 185 (2005); doi:10.1093/eurpub/cki163.
  3. A. Hasim, M. Ali, B. Mamtimin, J.Q. Ma, Q.Z. Li and A. Abudula, Exp. Ther. Med., 3, 945 (2012).
  4. E.S. Amirian, K. Adler-Storthz and M.E. Scheurer, Cancer Lett., 336, 18 (2013); doi:10.1016/j.canlet.2013.04.023.
  5. E.K. Yim and J.S. Park, Expert Rev. Proteomics, 3, 21 (2006); doi:10.1586/14789450.3.1.21.
  6. N.S. Ye and J. Li, Anal. Lett., 46, 250 (2013); doi:10.1080/00032719.2012.718826.
  7. G.A.N. Gowda, S. Zhang, H. Gu, V. Asiago, N. Shanaiah and D. Raftery, Expert Rev. Mol. Diagn., 8, 617 (2008); doi:10.1586/14737159.8.5.617.
  8. Y.S. Kim, P. Maruvada and J.A. Milner, Future Oncol., 4, 93 (2008); doi:10.2217/14796694.4.1.93.
  9. M. Yoshida, N. Hatano, S. Nishiumi, Y. Irino, Y. Izumi, T. Takenawa and T. Azuma, J. Gastroenterol., 47, 9 (2012); doi:10.1007/s00535-011-0493-8.
  10. J.L. Spratlin, N.J. Serkova and S.G. Eckhardt, Clin. Cancer Res., 15, 431 (2009); doi:10.1158/1078-0432.CCR-08-1059.
  11. G. Theodoridis, H.G. Gika and I.D. Wilson, Mass Spectrom. Rev., 30, 884 (2011); doi:10.1002/mas.20306.
  12. B. Sitter, T.F. Bathen, M.B. Tessem and I.S. Gribbestad, Prog. Nucl. Mag. Res. Sp., 54, 239 (2009); doi:10.1016/j.pnmrs.2008.10.001.
  13. D.I. Ellis and R. Goodacre, Analyst, 131, 875 (2006); doi:10.1039/b602376m.
  14. R. Ramautar, O.A. Mayboroda, G.W. Somsen and G.J. de Jong, Electrophoresis, 32, 52 (2011); doi:10.1002/elps.201000378.
  15. R. Ramautar, G.W. Somsen and G.J. de Jong, Electrophoresis, 30, 276 (2009); doi:10.1002/elps.200800512.
  16. R. Ramautar, G.W. Somsen and G.J. de Jong, Electrophoresis, 34, 86 (2013); doi:10.1002/elps.201200390.
  17. Z.M. Wu, Z.Q. Huang, R. Lehmann, C.X. Zhao and G.W. Xu, Chromatographia, 69, S23 (2009); doi:10.1365/s10337-009-0956-8.
  18. M.M. Koek, R.H. Jellema, J. Greef, A.C. Tas and T. Hankemeier, Metabolomics, 7, 307 (2011); doi:10.1007/s11306-010-0254-3.
  19. P.A. Guy, I. Tavazzi, S.J. Bruce, Z. Ramadan and S. Kochhar, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 871, 253 (2008); doi:10.1016/j.jchromb.2008.04.034.
  20. R. Llorach-Asunción, O. Jauregui, M. Urpi-Sarda and C. Andres-Lacueva, J. Pharm. Biomed. Anal., 51, 373 (2010); doi:10.1016/j.jpba.2009.06.033.
  21. D.A. Pirman, E. Efuet, X.P. Ding, Y. Pan, L. Tan, S.M. Fischer, R.N. DuBois and P. Yang, PLoS ONE, 8, e61379 (2013); doi:10.1371/journal.pone.0061379.
  22. J. Chen, Y.H. Shan, Q. Yan, S.M. Zhao, P.Y. Yin, X.J. Zhao, X. Lu, X.W. Xi, X.P. Wan and G.W. Xu, Sci. China Chem., 39, 1268 (2009).
  23. H.M. Woo, K.M. Kim, M.H. Choi, B.H. Jung, J. Lee, G. Kong, S.J. Nam, S. Kim, S.W. Bai and B.C. Chung, Clin. Chim. Acta, 400, 63 (2009); doi:10.1016/j.cca.2008.10.014.
  24. H. Wu, T.T. Liu, C.G. Ma, R.Y. Xue, C.H. Deng, H.Z. Zeng and X.Z. Shen, Anal. Bioanal. Chem., 401, 635 (2011); doi:10.1007/s00216-011-5098-9.
  25. O. Beckonert, M. E. Bollard, T.M.D. Ebbels, H.C. Keun, H. Antti, E. Holmes, J.C. Lindon and J.K. Nicholson, Anal. Chim. Acta, 490, 3 (2003); doi:10.1016/S0003-2670(03)00060-6.
  26. H. Wu, R.Y. Xue, C.L. Lu, C.H. Deng, T.T. Liu, H.Z. Zeng, Q. Wang and X. Shen, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 877, 3111 (2009); doi:10.1016/j.jchromb.2009.07.039.
  27. A. Ikeda, S. Nishiumi, M. Shinohara, T. Yoshie, N. Hatano, T. Okuno, T. Bamba, E. Fukusaki, T. Takenawa, T. Azuma and M. Yoshida, Biomed. Chromatogr., 26, 548 (2012); doi:10.1002/bmc.1671.
  28. J.D. Hu, H.Q. Tang, Q. Zhang, J. Fan, J. Hong, J.Z. Gu and J.L. Chen, World J. Gastroenterol., 17, 727 (2011); doi:10.3748/wjg.v17.i6.727.
  29. J. Wang, L.F. Yu, P. Shen and S.F. Wang, J. Zhejiang Univ. Med. Sci., 38, 478 (2009).
  30. H. Wu, R.Y. Xue, L. Dong, T.T. Liu, C.H. Deng, H.Z. Zeng and X.Z. Shen, Anal. Chim. Acta, 648, 98 (2009); doi:10.1016/j.aca.2009.06.033.
  31. Z.F. Li, J. Wang, C. Huang, S. Zhang, J. Yang, A. Jiang, R. Zhou and D. Pan, Rapid Commun. Mass Spectrom., 24, 2765 (2010); doi:10.1002/rcm.4703.
  32. H. Wu, R.Y. Xue, Z.Q. Tang, C.H. Deng, T.T. Liu, H.Z. Zeng, Y.H. Sun and X.Z. Shen, Anal. Bioanal. Chem., 396, 1385 (2010); doi:10.1007/s00216-009-3317-4.
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 0 Download : 1