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Vol. 27 No. 2 (2015): Vol 27 Issue 2

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Antioxidant and Cytotoxic Activities of Isolated Compounds from Ethyl Acetate Fraction of Alpinia oxyphylla Fruits

https://doi.org/10.14233/ajchem.2015.17012
Wei Fan
Shanghai Key Laboratory of Functional Materials Chemistry and Research Center of Analysis and Test, East China University of Science and Technology, Shanghai 200237, P.R. China
Cheng-Zhong Wang
Shanghai Key Laboratory of Functional Materials Chemistry and Research Center of Analysis and Test, East China University of Science and Technology, Shanghai 200237, P.R. China
Xiao-Li Bao
Shanghai Key Laboratory of Functional Materials Chemistry and Research Center of Analysis and Test, East China University of Science and Technology, Shanghai 200237, P.R. China
Hui-Hui Yuan
Shanghai Key Laboratory of Functional Materials Chemistry and Research Center of Analysis and Test, East China University of Science and Technology, Shanghai 200237, P.R. China
Min-Bo Lan
Shanghai Key Laboratory of Functional Materials Chemistry and Research Center of Analysis and Test, East China University of Science and Technology, Shanghai 200237, P.R. China

Corresponding Author(s) : Hui-Hui Yuan

yuanhuihui@ecust.edu.cn

Asian Journal of Chemistry, Vol. 27 No. 2 (2015): Vol 27 Issue 2

Abstract

A bioactivity-guided isolation and purification process was used to identify the antioxidant and cytotoxic components from the ethyl acetate fraction of Alpinia oxyphylla fruits. Four subfractions of the ethyl acetate fraction were obtained by a silica gel column chromatography eluted with petroleum ether-ethyl acetate, of which subfraction A showed the highest antioxidant and cytotoxic activities was selected for the isolation and identification of major active compounds. Seven compounds including nootkatone (1), tectochrysin (2), b-sitosterol (3), chrysin (4), protocatechuic acid (5), (4S*, 5E, 10R*)-7-oxo-tri-nor-eudesm-5-en-4b-ol (6) and (4R, 6R, 10S)-4,10-dimethyl-6-(1'-hydroxyisopropyl)-1-en-3,4,5,6,7,8-hexahydronaphthalen-2-one (7) were isolated. Compounds 6 and 7 were found firstly from this plant. Biological tests revealed that compounds 5, 6, 7 were the major antioxidant compounds and compounds 1, 3, 4, 5 exhibited potent cytotoxicity on different cancer cells, which probably responsible for the antioxidant and anticancer activities of the ethyl acetate fraction of Alpinia oxyphylla fruits.

Keywords

Alpinia oxyphylla Antioxidant activity Cytotoxicity Bioactive compounds
Fan, W., Wang, C.-Z., Bao, X.-L., Yuan, H.-H., & Lan, M.-B. (2015). Antioxidant and Cytotoxic Activities of Isolated Compounds from Ethyl Acetate Fraction of Alpinia oxyphylla Fruits. Asian Journal of Chemistry, 27(2), 532–536. https://doi.org/10.14233/ajchem.2015.17012
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