Preparative Separation and Purification of Flavonoids from Ephedra sinica Stapf. by High-Speed Counter-Current Chromatography

Lei Fang; Wenmao Geng; Jie Zhou; Ruimin Cao; Zhiwei Wang; Jialian Li; Yanling Geng

doi:10.14233/ajchem.2015.17007

Issue

Vol. 27 No. 2 (2015): Vol 27 Issue 2

Issue Published : January 10, 2015

This work is licensed under a Creative Commons Attribution 4.0 International License.

Preparative Separation and Purification of Flavonoids from Ephedra sinica Stapf. by High-Speed Counter-Current Chromatography

https://doi.org/10.14233/ajchem.2015.17007

Lei Fang

Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong Province, P.R. China

Wenmao Geng

Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, P.R. China

Jie Zhou

Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong Province, P.R. China

Ruimin Cao

Department of Pharmacy, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Rd, Jinan, Shandong, P.R. China

Zhiwei Wang

Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong Province, P.R. China

Jialian Li

Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong Province, P.R. China

Yanling Geng

Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong Province, P.R. China

Corresponding Author(s) : Lei Fang

fleiv@163.com

Asian Journal of Chemistry, Vol. 27 No. 2 (2015): Vol 27 Issue 2
Article Published : January 10, 2015

Abstract

Preparative high-speed counter-current chromatography was successfully performed for separation and purification of flavonoids from the stem of Ephedra sinica Stapf. with a two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water (0.5:3.5:1:3, v/v/v/v). This separation process produced four flavonoids, mahuannin A (1), mahuannin D (2), dihydroquercetin (3) and catechin (4) from 350 mg of crude sample in a one-step separation with purities of 98.8, 98.6, 98.3 and 98.2 %, respectively. The structures of these flavonoids were confirmed by ESI-MS, ¹H NMR and ¹³C NMR.

Keywords

High-speed counter-current chromatography Flavonoids Ephedra sinica Stapf

Fang, L., Geng, W., Zhou, J., Cao, R., Wang, Z., Li, J., & Geng, Y. (2015). Preparative Separation and Purification of Flavonoids from Ephedra sinica Stapf. by High-Speed Counter-Current Chromatography. Asian Journal of Chemistry, 27(2), 517–520. https://doi.org/10.14233/ajchem.2015.17007

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References

Editorial Committee of Chinese Materia Medica, Chinese Materia Medica, Shanghai Science & Technology Press, Shanghai, Vol. 6, p. 213 (2000).
The State Pharmacopoeia Commission of China, Pharmacopoeia of China, Chemical Industry Press, Beijing, p. 300 (2010).
P. Zheng, G.D. Dai and H.Q. Li, Ningxia Med. J., 24, 126 (2002).
Z.H. Xu, G.W. Qin, X.Y. Li and R.S. Xu, Acta Pharmacol. Sin., 36, 668 (2001).
Z. Xu, S.J. Jia, G.S. Tan and Y.J. Li, China J. Modern Med., 14, 88 (2004).
L. Xu and M.H.Yin, J. Medical Sci. Yanbian Univ., 32, 246 (2009).
X.K. Zheng, T.L. Ning, X.L. Wang, C.X. Liu, Y.Y. Liu and W.S. Feng, Chin. Pharm. J., 46, 998 (2011).
H.Z. Wu, Y. Lu, L.Q. Ai, X.W. Wu and Y.F. Yang, Asia-Pacific Tradit. Med., 4, 144 (2008).
L.J. Chen, D.E. Games and J. Jones, J. Chromatogr. A, 988, 95 (2003); doi:10.1016/S0021-9673(02)01954-4.
R.M. Liu, A.F. Li, A.L. Sun, J.C. Cui and L.Y. Kong, J. Chromatogr. A, 1064, 53 (2005); doi:10.1016/j.chroma.2004.12.026.
H.B. Li and F. Chen, J. Chromatogr. A, 1074, 107 (2005); doi:10.1016/j.chroma.2005.03.088.
X. Wang, Y.Q. Wang, Y.L. Geng, F.W. Li and C.C. Zheng, J. Chromatogr. A, 1036, 171 (2004); doi:10.1016/j.chroma.2004.02.073.
J.Y. Peng, G.R. Fan and Y.T. Wu, J. Chromatogr. A, 1091, 89 (2005); doi:10.1016/j.chroma.2005.07.072.
H. Oka, K.I. Harada, Y. Ito and Y. Ito, J. Chromatogr. A, 812, 35 (1998); doi:10.1016/S0021-9673(97)01277-6.
H. Oka, K.I. Harada, M. Suzuki and Y. Ito, J. Chromatogr. A, 903, 93 (2000); doi:10.1016/S0021-9673(00)00903-1.
H. Hikino, N. Shimoyama, Y. Kasahara, M. Takahashi and C. Konno, Heterocycles, 19, 1381 (1982); doi:10.3987/R-1982-08-1381.
H. Hikino and Y. Kasahara, Heterocycles, 20, 1953 (1983); doi:10.3987/R-1983-10-1953.
C.C. Shen, Y.S. Chang and L.K. Hott, Phytochemistry, 34, 843 (1993); doi:10.1016/0031-9422(93)85370-7.

References

Editorial Committee of Chinese Materia Medica, Chinese Materia Medica, Shanghai Science & Technology Press, Shanghai, Vol. 6, p. 213 (2000).

The State Pharmacopoeia Commission of China, Pharmacopoeia of China, Chemical Industry Press, Beijing, p. 300 (2010).

P. Zheng, G.D. Dai and H.Q. Li, Ningxia Med. J., 24, 126 (2002).

Z.H. Xu, G.W. Qin, X.Y. Li and R.S. Xu, Acta Pharmacol. Sin., 36, 668 (2001).

Z. Xu, S.J. Jia, G.S. Tan and Y.J. Li, China J. Modern Med., 14, 88 (2004).

L. Xu and M.H.Yin, J. Medical Sci. Yanbian Univ., 32, 246 (2009).

X.K. Zheng, T.L. Ning, X.L. Wang, C.X. Liu, Y.Y. Liu and W.S. Feng, Chin. Pharm. J., 46, 998 (2011).

H.Z. Wu, Y. Lu, L.Q. Ai, X.W. Wu and Y.F. Yang, Asia-Pacific Tradit. Med., 4, 144 (2008).

L.J. Chen, D.E. Games and J. Jones, J. Chromatogr. A, 988, 95 (2003); doi:10.1016/S0021-9673(02)01954-4.

R.M. Liu, A.F. Li, A.L. Sun, J.C. Cui and L.Y. Kong, J. Chromatogr. A, 1064, 53 (2005); doi:10.1016/j.chroma.2004.12.026.

H.B. Li and F. Chen, J. Chromatogr. A, 1074, 107 (2005); doi:10.1016/j.chroma.2005.03.088.

X. Wang, Y.Q. Wang, Y.L. Geng, F.W. Li and C.C. Zheng, J. Chromatogr. A, 1036, 171 (2004); doi:10.1016/j.chroma.2004.02.073.

J.Y. Peng, G.R. Fan and Y.T. Wu, J. Chromatogr. A, 1091, 89 (2005); doi:10.1016/j.chroma.2005.07.072.

H. Oka, K.I. Harada, Y. Ito and Y. Ito, J. Chromatogr. A, 812, 35 (1998); doi:10.1016/S0021-9673(97)01277-6.

H. Oka, K.I. Harada, M. Suzuki and Y. Ito, J. Chromatogr. A, 903, 93 (2000); doi:10.1016/S0021-9673(00)00903-1.

H. Hikino, N. Shimoyama, Y. Kasahara, M. Takahashi and C. Konno, Heterocycles, 19, 1381 (1982); doi:10.3987/R-1982-08-1381.

H. Hikino and Y. Kasahara, Heterocycles, 20, 1953 (1983); doi:10.3987/R-1983-10-1953.

C.C. Shen, Y.S. Chang and L.K. Hott, Phytochemistry, 34, 843 (1993); doi:10.1016/0031-9422(93)85370-7.

Issue

Vol. 27 No. 2 (2015): Vol 27 Issue 2

Preparative Separation and Purification of Flavonoids from Ephedra sinica Stapf. by High-Speed Counter-Current Chromatography

Corresponding Author(s) : Lei Fang

Abstract

Keywords

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