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HPLC Analysis of Sinapine Thiocyanate in Raphanus sativus and Determination of Its Involvement in Gastric-Intestinal Function
Corresponding Author(s) : Pingfang Tian
Asian Journal of Chemistry,
Vol. 27 No. 1 (2015): Vol 27 Issue 1
Abstract
The sinapine thiocyanate from the fresh and cooked juice of Raphanus sativus was analyzed by a high-performance liquid chromatographic (HPLC) system equipped with a C18 column and an UV detector. The column temperature was 25 ºC. The mobile phase was acetonitrile and 3 % acetic acid (10: 90) at a flow rate of 0.8 mL/min. The sinapine thiocyanate in fresh and cooked juice was 3.33 and 1.49 μg/g, respectively. It is notable that the cooked juice of radish promoted gastrointestinal transit, while the fresh juice exerted remarkable impact on gastric emptying. We thus reason that sinapine thiocyanate may be positively correlated with gastric emptying, yet has little impact on gastrointestinal transit. This study provides basis for both food processing and in-depth exploration of the roles of radish ingredients in ameliorating gastrointestinal transit.
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References
J.J. Wang, L.N. Liu, Q. Ming and D.Y. Xia, Chin. J. Gastroenterol. Hepatol., 116, 256 (2007).
Q.D. Jiang, J.J. Wang and L.N. Liu, J. Shanxi Med. Univ., 38, 215 (2007).
L. Zhao, L.W. Lu and X.Y. Li, Agric. Sci. China, 7, 823 (2008).
E. Capecka, Acta Physiol. Plant., 20, 143 (1998); doi:10.1007/s11738-998-0005-5.
U. Thiyam, H. Stöckmann, T. Zum Felde and K. Schwarz, Eur. J. Lipid Sci. Technol., 108, 239 (2006); doi:10.1002/ejlt.200500292.
J.F. Xing, W.Y. Feng and J.Y. Hou, J. Beijing Univ. TCM, 26, 50 (2003).
A.H. Gilani and M.N. Ghayur, J. Ethnopharmacol., 95, 169 (2004); doi:10.1016/j.jep.2004.06.038.
H.J. Han and H.J. Kwon, J. Toxicol. Environ. Health A, 72, 1380 (2009); doi:10.1080/15287390903212709.
Z.L. Lu, L.W. Liu, X.Y. Li, Y.Q. Gong, X.L. Hou, X.W. Zhu, J.L. Yang and L.Z. Wang, Agric. Sci. China, 7, 823 (2008); doi:10.1016/S1671-2927(08)60119-4.
K.Y. Jung, Y.K. Choo, H.M. Kim and B.K. Choi, Pharm. Pharmacol., 52, 1031 (2000); doi:10.1211/0022357001774769.
S.I. Jeong, S. Lee, B.K. Choi, K.Y. Jung, K.J. Kim, K.S. Keum and Y.K. Choo, J. Pharm. Pharmacol., 57, 1653 (2005); doi:10.1211/jpp.57.12.0016.
F. Dehghani and M. Panjehshahin, Toxicol. Lett., 180S, S130 (2008); doi:10.1016/j.toxlet.2008.06.752.