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Vol. 27 No. 12 (2015): Vol 27 Issue 12

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Antifungal, Antibacterial, Phytotoxic and Insecticidal Activities of Nigella sativa Seeds

https://doi.org/10.14233/ajchem.2015.19246
Amina Rahat
College of Home Economics, University of Peshawar, Peshawar, Pakistan
Shumaila Bashir
Department of Pharmacy, University of Peshawar, Peshawar, Pakistan
Khanzadi Fatima Khattak
Department of Chemistry, Abdul Wali Khan University, Mardan, Pakistan
Bashir Ahmad
Center of Biotechnology and Microbiology, University of Peshawar, Peshawar, Pakistan
Ibrar Khan
Center of Biotechnology and Microbiology, University of Peshawar, Peshawar, Pakistan
Sadiq Azam
Center of Biotechnology and Microbiology, University of Peshawar, Peshawar, Pakistan
Javid Ali
PCSIR Laboratories Complex, Jamrud Road, Peshawar, Pakistan
Jamshaid Ahmad
Center of Biotechnology and Microbiology, University of Peshawar, Peshawar, Pakistan

Corresponding Author(s) : Shumaila Bashir

saadb23@yahoo.com; saidhassan15@yahoo.com

Asian Journal of Chemistry, Vol. 27 No. 12 (2015): Vol 27 Issue 12

Abstract

The crude methanolic extract and different fractions (n-hexane, chloroform, ethyl acetate) of Nigella sativa seeds were screened for antifungal, antibacterial, phytotoxic and insecticidal activities. The methanolic extract showed total inhibitions against Escherichia coli, Proteus mirabilis, Bacillus cereus and Xanthomonas oryzae at 3 mg/mL. The n-hexane fraction showed significant antibacterial activity against P. mirabilis, B. cereus and X. oryzae, while CHCl3 fraction had 100 % inhibition against P. mirabilis, X. oryzae and E. coli. Chloroform fraction possessed significant antifungal activity against Penicillium oxalicum, Acremonium strictum, Verticillium lecanii and Trichoderma harzianum. The methanolic extract exhibited significant activity (92 %) against P. oxalicum, good activity (64 %) against A. strictum and moderate activity (56 %, 42 %) against V. lecanii and T. harizinum respectively. The n-hexane, aqueous and EtOAc fractions were also found active against most of the test fungi. The methanolic extract showed good (75 %) phytotoxic activity against Lemna minor, while EtOAc and n-hexane fractions showed no phytotoxicity. Chloroform fraction showed 70 % mortality of Tribolium castaneum, while EtOAc and n-hexane fractions showed no activity against T. castaneum.

Keywords

Nigella sativa Antibacterial Antifungal Phytotoxic Insecticidal
Rahat, A., Bashir, S., Fatima Khattak, K., Ahmad, B., Khan, I., Azam, S., … Ahmad, J. (2015). Antifungal, Antibacterial, Phytotoxic and Insecticidal Activities of Nigella sativa Seeds. Asian Journal of Chemistry, 27(12), 4591–4594. https://doi.org/10.14233/ajchem.2015.19246
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