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Vol. 27 No. 12 (2015): Vol 27 Issue 12

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Evaluation of Terminalia bellerica Roxb. Leaf Extracted in Different Solvents for Antioxidant Activities

https://doi.org/10.14233/ajchem.2015.19208
L. Sherin
Department of Chemical Engineering, COMSATS Institute of Information Technology, Defence Road, Off Raiwind Road, Lahore, Pakistan
M. Mustafa
Department of Chemical Engineering, COMSATS Institute of Information Technology, Defence Road, Off Raiwind Road, Lahore, Pakistan
S. Shujaat
Department of Chemistry, Lahore College for Women University, Lahore -54000, Pakistan

Corresponding Author(s) : L. Sherin

lubnasherin@ciitlahore.edu.pk

Asian Journal of Chemistry, Vol. 27 No. 12 (2015): Vol 27 Issue 12

Abstract

Terminalia bellerica, an esteemed ayurvedic plant, is employed traditionally in the management of an array of diverse pathological conditions. The present work is aimed to assess antioxidant potential of plant leaf extracts. Crude methanol extract was fractioned with different solvents and antioxidant activities along with total phenolic and flavonoid content were investigated with different antioxidant testing models, including DPPH, ABTS, anti-lipid peroxidation and total antioxidant capacity assays. Ethyl acetate (IC50 = 3.48 μg mL-1) as well as chloroform (IC50 = 4.55 μg mL-1) extracts exhibited persuasive DPPH radical scavenging activity, much better than standard antioxidant Trolox (IC50 = 6.17 μg mL-1). In ABTS assay, ethyl acetate extract (IC50 = 0.18 μg mL-1) showed enhanced potential in comparison to other extracts and standard antioxidants Trolox and n-propyl gallate. Chloroform extract offered maximum protection against lipid peroxidation (IC50 = 0.28 mg mL-1) and highest total antioxidant activity (4.54 mM g-1). All the extracts showed variable magnitude of phenolics and flavonoids content. In conclusion, ethylacetate and chloroform extracts of Terminalia bellerica leaf possess potent antioxidant potential which could be harnessed as economically viable source of natural antioxidants.

Keywords

Terminalia bellerica leaf Antioxidant activity Anti-lipid peroxidation DPPH assay ABTS assay
Sherin, L., Mustafa, M., & Shujaat, S. (2015). Evaluation of Terminalia bellerica Roxb. Leaf Extracted in Different Solvents for Antioxidant Activities. Asian Journal of Chemistry, 27(12), 4527–4531. https://doi.org/10.14233/ajchem.2015.19208
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