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Vol. 27 No. 12 (2015): Vol 27 Issue 12

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Spectrophotometric Determination of Procaine Hydrochloride in Pharmaceutical Preparations via Diazotization-Coupling Reaction with Phenol

https://doi.org/10.14233/ajchem.2015.19169
Wasan A. Al-Uzri
Chemistry Department, College of Science, Baghdad University, Al-Jadiriya, Baghdad, Iraq

Corresponding Author(s) : Wasan A. Al-Uzri

wasanuzri67@yahoo.com

Asian Journal of Chemistry, Vol. 27 No. 12 (2015): Vol 27 Issue 12

Abstract

A simple, rapid and sensitive spectrophotometric method has been developed for determination of procaine hydrochloride in pure form and injections. The proposed method is based on coupling reaction between diazotized procaine hydrochloride with phenol in alkaline medium to form an intense yellow, water-soluble dye that is stable and has a maximum absorption at 450 nm. The calibration graph was linear over the concentration range of 2 to 22 μg mL-1 with a molar absorbtivity of 2.469 × 104 L mol-1 cm-1. Sandell’s sensitivity of 11.045 × 10-3 μg mL-1. The optimum conditions for full colour development are described and the proposed method was probably applied satisfactorily to pharmaceutical injections.

Keywords

Procaine HCl Spectrophotometry Phenol Diazotization coupling reaction
A. Al-Uzri, W. (2015). Spectrophotometric Determination of Procaine Hydrochloride in Pharmaceutical Preparations via Diazotization-Coupling Reaction with Phenol. Asian Journal of Chemistry, 27(12), 4449–4453. https://doi.org/10.14233/ajchem.2015.19169
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