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Oil Palm Trunk of Lignocellulolytic Resources as Raw Material for Production of Fermentable Sugars via Enzymatic Hydrolysis Using Cellulose Thermostable
Corresponding Author(s) : Yetti Marlida
Asian Journal of Chemistry,
Vol. 27 No. 11 (2015): Vol 27 Issue 11
Abstract
This study aimed to explore the optimization of the concentration of oil palm trunks and thermostable cellulose enzyme preformance produce highest sugar (glucose total and reducing sugars) as well as the degree of polymerization. The design used in this study was a completely randomized design (CRD) factorial using 2 factors: factor A consists of four levels of cellulose enzyme that A1: 250 U/kg, A2: 500 U/kg, A3: 750 U/kg and A4: 1000 U/kg. Factor B is the concentration of oil palm trunk yitu B1: 20 % ; B2: 40 % and B3: 60 % were repeated 3 times. The results showed that there is a significantly effect (P < 0.05) in the levels of cellulose enzyme (factor A) with the concentration of oil palm trunks (factor B) and interactions of enzyme and oil palm trunk (factor AB) to reducing sugar, total sugars and degree of polymerization. This research can be concluded that the best total sugar produced was 19.68 mg/mL, the reducing sugars was 11.17 and degree of polymerization was 3.47.
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- Malaysian Oil Palm Statistics 2012, Malaysia Palm Oil Board, Ministry of Plantation & Commodities, Kajang, edn 32 (2013).
- S.P. Sumathi, S.P. Chai and A.R. Mohamed, Renew. Sustain. Energy Rev., 12, 2404 (2008); doi:10.1016/j.rser.2007.06.006.
- M.Z.A.A. Alam, A.A. Mamun, I.Y. Qudsieh, S.A. Muyibi, H.M. Salleh and N.M. Omar, Biochem. Eng. J., 46, 61 (2009); doi:10.1016/j.bej.2009.03.010.
- R.L.S. Hashim, L. How, R. Kumar and O. Sulaiman, Bioresour. Technol., 96, 1826 (2005); doi:10.1016/j.biortech.2005.01.023.
- M.H.P.S. Jawaid, H.P.S. Abdul Khalil, P. Noorunnisa Khanam and A. Abu Bakar, J. Polym. Environ., 19, 106 (2011); doi:10.1007/s10924-010-0203-2.
- M. Yetti, A. Syukri and N. Haska, Potency of Oil Palm Trunk as Feedstock for the Production of Bioethanol by Enzymatic Hydrolysis, MP3EI Reports (2012).
- J. Rafidah, I.W. Asma, E. Puad, S.M.A. Mahanim and H. Shaharuddin, Towards Zero Waste Production of Value Added Products from Waste Oil Palm Trunk (2011).
- K.L. Chin, P.S. H’ng, L.J. Wong, B.T. Tey and M.T. Paridah, Appl. Energy, 88, 4222 (2011); doi:10.1016/j.apenergy.2011.05.001.
- K.O. Lim, F.H. Ahmaddin and S.M. Vizhi, Bioresour. Technol., 59, 33 (1997); doi:10.1016/S0960-8524(96)00131-9.
- M. Yetti, A. Syukri and N. Haska, Application of Oil Palm Trunk as Feedstock for the Production of Bioethanol by Enzymatic Hydrolysis in Pilot Plan Scale Up, MP3EI Reports (2013).
- C. Breuil and J.N. Saddler, Enzyme Microb. Technol., 7, 327 (1985); doi:10.1016/0141-0229(85)90111-5.
- R.G. Steel and J.H. Torrie, Principles and Procedures of Statistics, McGraw Hill Book Co., NY, edn 2, pp. 633 (1980).
- T. Anindyawati, Enzymes and Prospects Leguminos Waste for the Production of Bioethanol, Research Center Bioeteknologi– LIPI Bandung, Indonesia (2009).
- D. Fengel and G. Wegener, Wood Chemistry, Ultrastucture, Reaction, Walter deGruyter, Berlin, New York (1984).
- K. Akihiko, T. Ryohei, S. Othman, H. Rokiah and A. Zubaidah, A.H. Mohd, N.M.Y. Wan, A.I. Takamitsu and M. Yoshinori, N. Satoru and M. Yutaka, 7th Biomass Asia Workshop, November 29 – December 1, Jakarta, Indonesia (2010).
- M. Yetti, Ph.D. Thesis, Isolation and Purification of Raw Starch Degrading Enzyme from Acreomonium Endopytic Fungus and its Aplication for Glucose Production, University Putra Malaysia, Malaysia (2001).
- F. Anggraini, Ph.D. Thesis, Assess an Extraction and Hydrolysis sxilandari barrel cabbage corn (Zea mays), Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor, Indonesia (2003).
References
Malaysian Oil Palm Statistics 2012, Malaysia Palm Oil Board, Ministry of Plantation & Commodities, Kajang, edn 32 (2013).
S.P. Sumathi, S.P. Chai and A.R. Mohamed, Renew. Sustain. Energy Rev., 12, 2404 (2008); doi:10.1016/j.rser.2007.06.006.
M.Z.A.A. Alam, A.A. Mamun, I.Y. Qudsieh, S.A. Muyibi, H.M. Salleh and N.M. Omar, Biochem. Eng. J., 46, 61 (2009); doi:10.1016/j.bej.2009.03.010.
R.L.S. Hashim, L. How, R. Kumar and O. Sulaiman, Bioresour. Technol., 96, 1826 (2005); doi:10.1016/j.biortech.2005.01.023.
M.H.P.S. Jawaid, H.P.S. Abdul Khalil, P. Noorunnisa Khanam and A. Abu Bakar, J. Polym. Environ., 19, 106 (2011); doi:10.1007/s10924-010-0203-2.
M. Yetti, A. Syukri and N. Haska, Potency of Oil Palm Trunk as Feedstock for the Production of Bioethanol by Enzymatic Hydrolysis, MP3EI Reports (2012).
J. Rafidah, I.W. Asma, E. Puad, S.M.A. Mahanim and H. Shaharuddin, Towards Zero Waste Production of Value Added Products from Waste Oil Palm Trunk (2011).
K.L. Chin, P.S. H’ng, L.J. Wong, B.T. Tey and M.T. Paridah, Appl. Energy, 88, 4222 (2011); doi:10.1016/j.apenergy.2011.05.001.
K.O. Lim, F.H. Ahmaddin and S.M. Vizhi, Bioresour. Technol., 59, 33 (1997); doi:10.1016/S0960-8524(96)00131-9.
M. Yetti, A. Syukri and N. Haska, Application of Oil Palm Trunk as Feedstock for the Production of Bioethanol by Enzymatic Hydrolysis in Pilot Plan Scale Up, MP3EI Reports (2013).
C. Breuil and J.N. Saddler, Enzyme Microb. Technol., 7, 327 (1985); doi:10.1016/0141-0229(85)90111-5.
R.G. Steel and J.H. Torrie, Principles and Procedures of Statistics, McGraw Hill Book Co., NY, edn 2, pp. 633 (1980).
T. Anindyawati, Enzymes and Prospects Leguminos Waste for the Production of Bioethanol, Research Center Bioeteknologi– LIPI Bandung, Indonesia (2009).
D. Fengel and G. Wegener, Wood Chemistry, Ultrastucture, Reaction, Walter deGruyter, Berlin, New York (1984).
K. Akihiko, T. Ryohei, S. Othman, H. Rokiah and A. Zubaidah, A.H. Mohd, N.M.Y. Wan, A.I. Takamitsu and M. Yoshinori, N. Satoru and M. Yutaka, 7th Biomass Asia Workshop, November 29 – December 1, Jakarta, Indonesia (2010).
M. Yetti, Ph.D. Thesis, Isolation and Purification of Raw Starch Degrading Enzyme from Acreomonium Endopytic Fungus and its Aplication for Glucose Production, University Putra Malaysia, Malaysia (2001).
F. Anggraini, Ph.D. Thesis, Assess an Extraction and Hydrolysis sxilandari barrel cabbage corn (Zea mays), Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor, Indonesia (2003).