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Investigation of Solvent-Dependent Catalytic Behaviour of Hydrophobic Guest Artificial Glutathione Peroxidase Using H2O2 and 3-Carboxyl-4-nitrobenzenethiol as Substrates
Corresponding Author(s) : Y.Z. Yin
Asian Journal of Chemistry,
Vol. 27 No. 10 (2015): Vol 27 Issue 10
Abstract
The investigation of the catalytic behaviour of a hydrophobic guest artificial glutathione peroxidase (GPx) (ADA-Te-OH) was carried out employing H2O2 and 3-carboxyl-4-nitrobenzenethiol (TNB) as substrates. The relation between the catalytic rate of ADA-Te-OH and the property of solvent used in the determination of catalytic activity was revealed. Typically, the co-solvents including ethanol, DMSO, DMF and CH3CN were employed in the determination of catalytic rates. It indicated that ADA-Te-OH exhibited the typical solvent-dependent catalytic behaviour. Especially, higher catalytic rate was observed when polar protic solvent (ethanol) was used compared with other co-solvents. It suggested that polar protic solvent was the appropriate co-solvent for the assay of catalytic activity of hydrophobic artificial glutathione peroxidase. Additionally, the strong polarity of polar aprotic solvent plays an important role in the enhancement of glutathione peroxidase catalytic activity. This study embodies well understanding of the catalytic behaviour of hydrophobic guest artificial glutathione peroxidase.
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References
L. Flohé, G. Loschen, W.A. Günzler and E. Eichele, Hoppe Seylers Z. Physiol. Chem., 353, 987 (1972); doi:10.1515/bchm2.1972.353.1.987.
N. Ezirmik, S. Taysi, R. Celik, G. Celik, H.A. Alici, H. Turhan, M. Cesur and D. Keskin, Asian J. Chem., 20, 1950 (2008).
A. Cebi, E. Diraman and Z. Eren, Asian J. Chem., 21, 1359 (2009).
Y. Yin, L. Wang, H. Jin, C. Lv, S. Yu, X. Huang, Q. Luo, J. Xu and J. Liu, Soft Matter, 7, 2521 (2011); doi:10.1039/c0sm01081b.
Y. Yin, Z. Dong, Q. Luo and J. Liu, Prog. Polym. Sci., 37, 1476 (2012); doi:10.1016/j.progpolymsci.2012.04.001.
H. Sies and H. Masumoto, Adv. Pharmacol., 38, 229 (1996); doi:10.1016/S1054-3589(08)60986-2.
B.K. Sarma and G. Mugesh, J. Am. Chem. Soc., 127, 11477 (2005); doi:10.1021/ja052794t.
X. Zhang, H. Xu, Z. Dong, Y. Wang, J. Liu and J. Shen, J. Am. Chem. Soc., 126, 10556 (2004); doi:10.1021/ja048890w.
S. Yu, X. Huang, L. Miao, J. Zhu, Y. Yin, Q. Luo, J. Xu, J. Shen and J. Liu, Bioorg. Chem., 38, 159 (2010); doi:10.1016/j.bioorg.2010.03.001.
Y.Z. Yin, C. Lang, X.X. Hu, Z.F. Shi, Y. Wang, S.F. Jiao, C.X. Cai and J.Q. Liu, Russ. J. Bioorg. Chem., 40, 162 (2014); doi:10.1134/S1068162014010142.
Z.P. Wu and D. Hilvert, J. Am. Chem. Soc., 112, 5647 (1990); doi:10.1021/ja00170a043.