This work is licensed under a Creative Commons Attribution 4.0 International License.
Determination of Pesticides in Dead Honeybees
Corresponding Author(s) : A.I. Kozhushkevich
Asian Journal of Chemistry,
Vol. 35 No. 5 (2023): Vol 35 Issue 5, 2023
Abstract
Pesticide residues were analyzed in dead honeybees by gas chromatography-tandem mass spectrometry (GC-MS/MS). Sample preparation using QuEChERS was followed by purification on a mixture of PSA and C18 sorbents. The extraction efficiency of > 80% was obtained for all analytes and pesticides were quantified in the range of 5-1000 ng/g. This method was used for investigating incidents of honeybees poisoning by pesticides. Fipronil was detected in all of the investigated samples originating from different regions of Russia. Additionally, τ-fluvalinate was detected in three samples.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
- Ministry of Agriculture of the Russian Federation, State Registry of Pesticides and Agrochemicals Allowed for use in the Russian Federation; http://agroportal2.garant.ru:81/SESSION/PILOT/main.htm
- N. Nazarova, The Impact of Agrochemicals on Honeybees, Evolyutsiya Obrazovaniya Rossii, 4, 134 (2009) (In Russian).
- M. Laurent, P. Hendrikx, R. Thiéry and M. Ribière-Chabert, A Pan-European Epidemiological Study on Honeybee Colony Losses (2012-2014) Revealed Winter Colony Losses up to 32.4% and Seasonal Colony Losses up to 11.1%, Euroreference 2, March (2017).
- K.V. Lee, N. Steinhauer, K. Rennich, M.E. Wilson, D.R. Tarpy, D.M. Caron, R. Rose, K.S. Delaplane, K. Baylis, E.J. Lengerich, J. Pettis, J.A. Skinner, J.T. Wilkes, R. Sagili and D. vanEngelsdorp, Apidologie, 46, 292 (2015); https://doi.org/10.1007/s13592-015-0356-z
- S. Walorczyk and B. Gnusowski, J. Chromatogr. A, 1216, 6522 (2009); https://doi.org/10.1016/j.chroma.2009.07.045
- L. Wiest, A. Buleté, B. Giroud, C. Fratta, H. Pouliquen, O. Lambert, S. Amic and C. Arnaudguilhem, J. Chromatogr. A, 1218, 5743 (2011); https://doi.org/10.1016/j.chroma.2011.06.079
- Z. Barganska, M. Slebioda and J. Namiesnik, Molecules, 19, 2911 (2014); https://doi.org/10.3390/molecules19032911
- B. Morzycka, J. Chromatogr. A, 982, 267 (2002); https://doi.org/10.1016/S0021-9673(02)01505-4
- L. Pareja, M. Colazzo, A. Perez-Parada, S. Niell, L. Carrasco-Letelier, N. Besil, M.W. Cesio and H. Heinzen, Int. J. Environ. Res. Public Health, 8, 3844 (2011); https://doi.org/10.3390/ijerph8103844
- L. Rajski, A. Lozano, A. Ucles, C. Ferrer and A.R. Fernandez-Alba, J. Chromatogr. A, 1304, 109 (2013); https://doi.org/10.1016/j.chroma.2013.06.070
- EN 1528-4:1997 Fatty Food - Determination of Pesticides and Poly-chlorinated Biphenyls (PCBs) Part 4: Determination, Confirmatory Tests, Miscellaneous.
- S.J. Lehotay, K. Mastovska and A.R. Lightfield, J. AOAC Int., 88, 615 (2005); https://doi.org/10.1093/jaoac/88.2.615
- M. Anastassiades, E. Scherbaum, B. Tasdelen and D. štajnbaher, Eds.: H. Ohkawa, H. Miyagawa and P.W. Lee, Recent Developments in QuEChERS Methodology for Pesticide Multiresidue Analysis, In: Pesticide Chemistry: Crop Protection, Public Health, Environmental Safety, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, pp. 439-458 (2007).
- M. Anastassiades, S.J. Lehotay, D. Stajnbaher and F.J. Schenck, J. AOAC Int., 86, 412 (2003); https://doi.org/10.1093/jaoac/86.2.412
References
Ministry of Agriculture of the Russian Federation, State Registry of Pesticides and Agrochemicals Allowed for use in the Russian Federation; http://agroportal2.garant.ru:81/SESSION/PILOT/main.htm
N. Nazarova, The Impact of Agrochemicals on Honeybees, Evolyutsiya Obrazovaniya Rossii, 4, 134 (2009) (In Russian).
M. Laurent, P. Hendrikx, R. Thiéry and M. Ribière-Chabert, A Pan-European Epidemiological Study on Honeybee Colony Losses (2012-2014) Revealed Winter Colony Losses up to 32.4% and Seasonal Colony Losses up to 11.1%, Euroreference 2, March (2017).
K.V. Lee, N. Steinhauer, K. Rennich, M.E. Wilson, D.R. Tarpy, D.M. Caron, R. Rose, K.S. Delaplane, K. Baylis, E.J. Lengerich, J. Pettis, J.A. Skinner, J.T. Wilkes, R. Sagili and D. vanEngelsdorp, Apidologie, 46, 292 (2015); https://doi.org/10.1007/s13592-015-0356-z
S. Walorczyk and B. Gnusowski, J. Chromatogr. A, 1216, 6522 (2009); https://doi.org/10.1016/j.chroma.2009.07.045
L. Wiest, A. Buleté, B. Giroud, C. Fratta, H. Pouliquen, O. Lambert, S. Amic and C. Arnaudguilhem, J. Chromatogr. A, 1218, 5743 (2011); https://doi.org/10.1016/j.chroma.2011.06.079
Z. Barganska, M. Slebioda and J. Namiesnik, Molecules, 19, 2911 (2014); https://doi.org/10.3390/molecules19032911
B. Morzycka, J. Chromatogr. A, 982, 267 (2002); https://doi.org/10.1016/S0021-9673(02)01505-4
L. Pareja, M. Colazzo, A. Perez-Parada, S. Niell, L. Carrasco-Letelier, N. Besil, M.W. Cesio and H. Heinzen, Int. J. Environ. Res. Public Health, 8, 3844 (2011); https://doi.org/10.3390/ijerph8103844
L. Rajski, A. Lozano, A. Ucles, C. Ferrer and A.R. Fernandez-Alba, J. Chromatogr. A, 1304, 109 (2013); https://doi.org/10.1016/j.chroma.2013.06.070
EN 1528-4:1997 Fatty Food - Determination of Pesticides and Poly-chlorinated Biphenyls (PCBs) Part 4: Determination, Confirmatory Tests, Miscellaneous.
S.J. Lehotay, K. Mastovska and A.R. Lightfield, J. AOAC Int., 88, 615 (2005); https://doi.org/10.1093/jaoac/88.2.615
M. Anastassiades, E. Scherbaum, B. Tasdelen and D. štajnbaher, Eds.: H. Ohkawa, H. Miyagawa and P.W. Lee, Recent Developments in QuEChERS Methodology for Pesticide Multiresidue Analysis, In: Pesticide Chemistry: Crop Protection, Public Health, Environmental Safety, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, pp. 439-458 (2007).
M. Anastassiades, S.J. Lehotay, D. Stajnbaher and F.J. Schenck, J. AOAC Int., 86, 412 (2003); https://doi.org/10.1093/jaoac/86.2.412