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Vol. 35 No. 3 (2023): Vol 35 Issue 3, 2023

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Phytochemicals Profiling of Blue-Green Alga Nostoc sp. HANL07: Antioxidant, Antibacterial Activity and GC-MS Analysis

https://doi.org/10.14233/ajchem.2023.27281
Ritu Chauhan
Amity Institute of Environmental Sciences, Amity University, Sector-125, Noida-201303, India
Ashutosh Tripathi
Amity Institute of Environmental Sciences, Amity University, Sector-125, Noida-201303, India
Abhishek Chauhan
Amity Institute of Environmental Toxicology, Safety and Management, Amity University, Sector-125, Noida-201303, India
Anuj Ranjan
Academy of Biology and Biotechnology, Southern Federal University, 344006 Rostov-on-Don, Russia
Hardeep Singh Tuli
Department of Biotechnology, Maharishi Markandeshwar Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala-133207, India
Hadi Sajid Abdulabbas
Continuous Education Department, Faculty of Dentistry, University of Al-Ameed, Karbala 56001, Iraq
Subhash C. Chauhan
STCECR, School of Medicine, University of Texas Rio Grande Valley, McAllen, TX 78504, U.S.A
Tanu Jindal
Amity Institute of Environmental Toxicology, Safety and Management, Amity University, Sector-125, Noida-201303, India

Corresponding Author(s) : Ashutosh Tripathi

atripathi1@amity.edu

Asian Journal of Chemistry, Vol. 35 No. 3 (2023): Vol 35 Issue 3, 2023

Abstract

In recent years, studies on phytochemicals, antioxidants, and antibiotics using blue green algae from harsh environments has become increasingly prevalent. The search for novel bioactive molecules exhibiting diverse biological activity is one of the most important strategies for addressing the issue of rising concerns regarding bacterial antibiotic resistance. In present study, the phytochemicals, antioxidant potentiality and antibacterial assessment of organic solvent extracts of blue-green alga isolated from Naini lake water were characterized. From the mass culture of alga, extracts were prepared in MeOH, EtOH, acetone, AcOEt, hexane and DCM and antioxidant potential and antibacterial properties were studied against multiple antibiotic-resistant E. coli, P. aeruginosa, P. fluorescence, S. enteric, S. typhimurium and Sh. dysenteriae. The 16S rRNA gene sequence analysis and phylogenetic tree revealed that blue-green algal isolate belongs to Nostoc sp. A higher extraction yield (5.3%) was recorded for MeOH extract as compared to other solvent extracts. Alkaloids, flavonoids and phenols were present in all extracts. DPPH free radical scavenging activity was determined using various concentrations of extracts (1 to 2048 μg mL-1). Maximum % inhibition (free radical scavenging activity) was observed in MeOH extract (35.23 ± 0.12). The methanolic extract was found to be inhibitory against P. aeruginosa and P. fluorescence. 2048 μg mL-1 MIC value was recorded against P. aeruginosa. In GC-MS profiling, a total of 8 significant peaks showing various compounds were identified among the 21 peaks. Oxirane hexadecyl was reported as the main components in this compound. The results showed that Nostoc sp. HANL07 is a good source of antioxidant and antibacterial compounds and could be used for further study in the field of drug discovery.

Keywords

Phytochemicals Phenolic compounds Flavonoids Antibiotic resistance Blue-green algae GC-MS analysis
Chauhan, R., Tripathi, A., Chauhan, A., Ranjan, A., Singh Tuli, H., Sajid Abdulabbas, H., … Jindal, T. (2023). Phytochemicals Profiling of Blue-Green Alga Nostoc sp. HANL07: Antioxidant, Antibacterial Activity and GC-MS Analysis. Asian Journal of Chemistry, 35(3), 755–762. https://doi.org/10.14233/ajchem.2023.27281
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