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Vol. 35 No. 1 (2023): Vol 35 Issue 1

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Enhanced Anticancer Property of Polyphenol-Assisted Biogenic AuNPs/RGO Nanocomposites in Triple-Negative Breast Cancer Cells

https://doi.org/10.14233/ajchem.2023.26937
R. Prasannadevi
Department of Physics, Annamalai University, Annamalainagar-608002, India
T. Radhiga
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar-608002, India
N. Rajendra Prasad
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar-608002, India
S. Dhanap
1Department of Physics, Annamalai University, Annamalainagar-608002, India 2Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar-608002, India 3School of Physics, Madurai Kamaraj University, Madurai-625021, India *Corresponding author: E-mail: nskumarphyamu@gmail.com
N. Krishnakumar
Department of Physics, Annamalai University, Annamalainagar-608002, India ; School of Physics, Madurai Kamaraj University, Madurai-625021, India

Corresponding Author(s) : N. Krishnakumar

nskumarphyamu@gmail.com

Asian Journal of Chemistry, Vol. 35 No. 1 (2023): Vol 35 Issue 1

Abstract

The bio-inspired nanocomposites developed from reduced graphene oxide (RGO) and gold nanoparticles (AuNPs) might be of great significance due to their exceptional physico-chemical properties. In the present work, the AuNPs/RGO nanocomposites were prepared by a green-synthesis approach using grape seed proanthocyanidin (GSP), which acts as a natural reducing and stabilizing agent. The simultaneous biosynthesis of RGO nanosheets from graphene oxide (GO) and AuNPs from chloroauric acid ions by GSP leads to the development of AuNPs/RGO nanocomposites, which were further confirmed by various analytical techniques. The analyses of the observed outcomes confirm the removal of oxygen containing moieties during the transformation process of GO to RGO. Further, AuNPs were decorated orderly on both sides of the RGO nanosheet surface with an average particle size of ~ 35 nm. The MTT-based cytotoxicity assay revealed the enhanced anticancer activity of the prepared AuNPs/RGO nanocomposites (IC50 = 15.6 μg/mL) against triple-negative breast cancer cells when compared to either AuNPs or RGO treatment alone. Moreover, enhanced ROS levels and subsequent alteration in mitochondrial membrane potential (Δψm) with nuclear fragmentation and apoptotic features were observed in AuNPs/RGO nanocomposites treated cells. This could be due to the synergistic effect of AuNPs/RGO in the triple-negative breast cancer cells.

Keywords

Gold nanoparticles Reduced graphene oxide Nanocomposites Grape seed proanthocyanidin Breast cancer Cytotoxicity
Prasannadevi, R., Radhiga, T., Rajendra Prasad, N., Dhanap, S., & Krishnakumar, N. (2022). Enhanced Anticancer Property of Polyphenol-Assisted Biogenic AuNPs/RGO Nanocomposites in Triple-Negative Breast Cancer Cells. Asian Journal of Chemistry, 35(1), 143–152. https://doi.org/10.14233/ajchem.2023.26937
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