Antibacterial Polyketide from Lasiodiplodia theobromae and Pyrenula bahiana on Mangrove Ecosystems in Puttalam Lagoon, Sri Lanka

P.A. Paranagama; S. Santhirasegaram; S.R. Wickramarachchi; R.N. Attanayake; G. Weerakoon; K. Maduranga

doi:10.14233/ajchem.2022.23789

Issue

Vol. 34 No. 11 (2022): Vol 34 Issue 11, 2022

Issue Published : October 19, 2022

This work is licensed under a Creative Commons Attribution 4.0 International License.

Antibacterial Polyketide from Lasiodiplodia theobromae and Pyrenula bahiana on Mangrove Ecosystems in Puttalam Lagoon, Sri Lanka

https://doi.org/10.14233/ajchem.2022.23789

P.A. Paranagama

Department of Chemistry, University of Kelaniya, Kelaniya, Sri Lanka

S. Santhirasegaram

Department of Chemistry, University of Kelaniya, Kelaniya, Sri Lanka

S.R. Wickramarachchi

Department of Chemistry, University of Kelaniya, Kelaniya, Sri Lanka

R.N. Attanayake

Department of Plant and Molecular Biology, University of Kelaniya, Kelaniya, Sri Lanka

G. Weerakoon

Algae, Fungi and Plants Division, Department of Life Sciences, The Natural History Museum, Cromwell Road, London, SW7 5BD, U.K

K. Maduranga

Department of Chemistry, University of Kelaniya, Kelaniya, Sri Lanka

Corresponding Author(s) : P.A. Paranagama

priyani@kln.ac.lk

Asian Journal of Chemistry, Vol. 34 No. 11 (2022): Vol 34 Issue 11, 2022
Article Published : October 19, 2022

Abstract

Lasiodiplodia theobromae is one of the frequently isolated fast growing endolichenic fungus. This fungus was isolated from the lichen host, Pyrenula bahiana collected from the mangrove ecosystems in Puttlam lagoon and its identification was confirmed based on rDNA-ITS sequence homology. Secondary metabolites of L. theobromae were extracted into ethyl acetate and subjected to antibacterial assay against Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC25923) and Bacillus subtilis (ATCC6051). Crude extract at a concentration of 6.8 μg/mL showed good antibacterial activity against the bacterial strain S. aureus compared with the activity of the standard azithromycin at a concentration of 5.0 μg/mL. Active crude extract was partitioned to obtain methanol, hexane and chloroform fractions. Chloroform fraction showed the highest activity to S. aureus out of three fractions. This fraction was subjected to bioassay-guided separation on silica gel column chromatography to isolate bioactive pure compounds. The bioactive pure compound was identified as (3R)-de-O-methyllasiodiplodin using LC-MS, 1D and 2D NMR spectroscopy.

Keywords

Endolichenic fungi Mangrove systems Antibacterial activity Staphylococcus aureus (3R)-de-O-methyllasiodiplodin

Paranagama, P., Santhirasegaram, S., Wickramarachchi, S., Attanayake, R., Weerakoon, G., & Maduranga, K. (2022). Antibacterial Polyketide from Lasiodiplodia theobromae and Pyrenula bahiana on Mangrove Ecosystems in Puttalam Lagoon, Sri Lanka. Asian Journal of Chemistry, 34(11), 2961–2964. https://doi.org/10.14233/ajchem.2022.23789

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References

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References

E. Ren, G. Strobel, J. Sears and M. Park, Microb. Ecol., 60, 130 (2010); https://doi.org/10.1007/s00248-009-9630-9

M.S. Butler, Nat. Prod. Rep., 22, 162 (2005); https://doi.org/10.1039/b402985m

M. Latha, K.M. Ramkumar, L. Pari, P.N. Damodaran, V. Rajeshkannan and T. Suresh, J. Med. Food, 9, 391 (2006); https://doi.org/10.1089/jmf.2006.9.391

P.A. Paranagama, E.M. Wijeratne, A.M. Burns, M.T. Marron, M.K. Gunatilaka, A.E. Arnold and A.A. Gunatilaka, J. Nat. Prod., 70, 1700 (2007); https://doi.org/10.1021/np070466w

H. Gao, J. Zou, J. Li and H. Zhao, Stud. Nat. Prod. Chem., 48, 347 (2016).

G. Weerakoon, Fascinating Lichens of Sri Lanka, Dilmah Conservations (2015).

K.A.U. Samanthi, S. Wickramarachchi, E.M.K. Wijeratne and P.A. Paranagama, J. Natl. Sci. Found. Sri Lanka, 43, 217 (2015); https://doi.org/10.4038/jnsfsr.v43i3.7950

K.A.U. Samanthi, S. Wickramaarachchi, E.M.K. Wijeratne and P.A. Paranagama, J. Natl. Sci. Found. Sri Lanka, 43, 119 (2015); https://doi.org/10.4038/jnsfsr.v43i2.7939

S.R. Wickramarachchi, U. Samanthi, K. Wijeratne and P.A. Paranagama, Int. J. Pharm. Sci. Res., 10, 3412 (2019); https://doi.org/10.13040/IJPSR.0975-8232.10(7).3412-20

P.C.Y. Woo, S.K.P. Lau, A.H.Y. Ngan, H. Tse, E.T.K. Tung and K.-Y. Yuen, J. Clin. Microbiol., 46, 380 (2008); https://doi.org/10.1128/JCM.01137-07

R. Chen, M.D. Willcox, K.K. Ho, D. Smyth and N. Kumar, Biomaterials, 85, 142 (2016); https://doi.org/10.1016/j.biomaterials.2016.01.063

S. Santhirasegaram, S.R. Wickramarachchi, R.N. Attanayake, G. Weerakoon, S. Samarakoon, K. Wijeratne and P.A. Paranagama, Nat. Prod. Commun., 15, 1934578X2093301 (2020); https://doi.org/10.1177/1934578X20933017

K. Maduranga, R.N. Attanayake, S. Santhirasegaram, G. Weerakoon and P.A. Paranagama, PLoS One, 13, e0200711 (2018); https://doi.org/10.1371/journal.pone.0200711

T.A. Hall, Nucleic Acids Symp. Ser., 41, pp. 95-98 (1999).

H.A.K. Maduranga, W.R.H. Weerasinghe, R.N. Attanayake, S. Santhirasegaram, C.D. Shevkar, A.S. Kate, G. Weerakoon, K.A.U. Samanthi, K. Kalia and P.A. Paranagama, Asian J. Chem., 33, 1425 (2021); https://doi.org/10.14233/ajchem.2021.23229

Issue

Vol. 34 No. 11 (2022): Vol 34 Issue 11, 2022

Antibacterial Polyketide from Lasiodiplodia theobromae and Pyrenula bahiana on Mangrove Ecosystems in Puttalam Lagoon, Sri Lanka

Corresponding Author(s) : P.A. Paranagama

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